Skip to main content
SpringerLink
Log in

Cloning and characterization of a novel human homolog* of mouse U26, a putative PQQ-dependent AAS dehydrogenase

  • Short communication
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Mouse U26 has been defined as a 2-aminoadipic 6-semialdehyde dehydrogenase. It was speculated to be a PQQ-dependent AAS dehydrogenase due to the research of demonstrating PQQ as a new B vitamin. We isolated a novel human cDNA from the human fetal brain cDNA library we constructed. Its deduced protein was most related to mouse U26. Thus, we termed it human U26. This putative protein contains an AMP-binding domain, a Phosphopantetheine-binding domain and six PQQ-binding motifs. Human U26 mRNA is ubiquitously expressed in adult tissues and is highly expressed in colon adenocarcinoma (CX-1) and colon adenocarcinoma (GI-112) cell lines. Further study should be made to clarify the precise function of human U26.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

AAS:

2-aminoadipic 6-semialdehyde

PQQ:

pyrroloquinoline quinone

Redox:

reduction-oxidation

CoA:

coenzyme A

PP/4’ PP:

phosphopantetheine, or pantetheine 4’ phosphate

ACP:

acyl carrier proteins

ACS:

acetyl-CoA synthetase

LACS:

Long-chain acyl-CoA synthetase

Fab:

fatty acid biosynthesis

Reference

  • T Kasahara T Kato (2003) Nature 422 832 Occurrence Handle1:CAS:528:DC%2BD3sXjtFCit7o%3D Occurrence Handle12712191

    CAS  PubMed  Google Scholar 

  • MJ Bibb DH Sherman S Omura DA Hopwood (1994) Gene 142 31–39 Occurrence Handle1:CAS:528:DyaK2cXlt1Knurg%3D Occurrence Handle8181754

    CAS  PubMed  Google Scholar 

  • DH Mallonee WB White PB Hylemon (1990) J. Bacteriol 172 7011–7019 Occurrence Handle1:CAS:528:DyaK3MXisVWntbk%3D Occurrence Handle2254270

    CAS  PubMed  Google Scholar 

  • J Schroeder (1989) Nucleic Acids Res 17 460–460 Occurrence Handle1:CAS:528:DyaL1MXptFanug%3D%3D Occurrence Handle2911486

    CAS  PubMed  Google Scholar 

  • DJ Smith AJ Earl G Turner (1990) EMBO J 9 2743–2750 Occurrence Handle1:CAS:528:DyaK3MXitVagu7s%3D Occurrence Handle2118102

    CAS  PubMed  Google Scholar 

  • LT Webster (1965) J. Biol. Chem 240 4164–4169 Occurrence Handle1:CAS:528:DyaF2MXkslelsrY%3D Occurrence Handle5845819

    CAS  PubMed  Google Scholar 

  • H Toh (1991) Protein Seq. Data Anal 4 111–117 Occurrence Handle1:CAS:528:DyaK3MXmsFSqtLk%3D Occurrence Handle1946328

    CAS  PubMed  Google Scholar 

  • PH Groot HR Scholte WC Hulsmann (1976) Adv. Lipid Res 14 75–126 Occurrence Handle1:CAS:528:DyaE28XktVWmt7c%3D Occurrence Handle3952

    CAS  PubMed  Google Scholar 

  • A Roujeinikova C Baldock WJ Simon J Gilroy PJ Baker AR Stuitje DW Rice AR Slabas JB Rafferty (2002) Structure (Camb) 10 IssueID6 825–835 Occurrence Handle1:CAS:528:DC%2BD38XksFOisr0%3D

    CAS  Google Scholar 

  • EL Pugh SJ Wakil (1965) J. Biol. Chem 240 4727–4733 Occurrence Handle1:CAS:528:DyaF2MXltVShtb0%3D Occurrence Handle5321311

    CAS  PubMed  Google Scholar 

  • HC Wong G Liu YM Zhang CO Rock J Zheng (2002) J. Biol. Chem 277 15874–15880 Occurrence Handle1:CAS:528:DC%2BD38Xjs1Wjtr8%3D Occurrence Handle11825906

    CAS  PubMed  Google Scholar 

  • JA Duine JA Jongejan (1989) Annu. Rev. Biochem 58 403–426 Occurrence Handle1:CAS:528:DyaL1MXkslentro%3D Occurrence Handle2549854

    CAS  PubMed  Google Scholar 

  • PM Gallop MA Paz R Fluckiger HM Kagan (1989) Trends Biochem Sci 14 IssueID8 343–346 Occurrence Handle1:CAS:528:DyaL1MXls12ksbk%3D Occurrence Handle2572081

    CAS  PubMed  Google Scholar 

  • J Killgore C Smidt L Duich N Romero-Chapman D Tinker K Reiser M Melko D Hyde RB Rucker (1989) Science 245 850–852 Occurrence Handle1:CAS:528:DyaL1MXlslartrY%3D Occurrence Handle2549636

    CAS  PubMed  Google Scholar 

  • FM Steinberg ME Gershwin RB Rucker (1994) J. Nutr 124 744–753 Occurrence Handle1:CAS:528:DyaK2cXjt1Wqtbo%3D Occurrence Handle8169668

    CAS  PubMed  Google Scholar 

  • Goodwin PM & Anthony C (1998) In: Poole RK (Ed) Advances in Microbial Physiology. (Vol. 40, pp 11–80), Academic Press, London, UK.

  • Z Xia W Dai J Xiong Z Hao VL Davidson S White FS Mathews (1992) J. Biol. Chem 267 22289–22297 Occurrence Handle1:CAS:528:DyaK38XlvVOhtr0%3D Occurrence Handle1331050

    CAS  PubMed  Google Scholar 

  • M Ghosh C Anthony K Harlos MG Goodwin CCF Blake (1995) Structure 3 177–187 Occurrence Handle1:CAS:528:DyaK2MXltlGmt7w%3D Occurrence Handle7735834

    CAS  PubMed  Google Scholar 

  • Z Xia W Dai Y Zhang SA White GD Boyd FS Mathews (1996) J. Mol. Biol 259 480–501 Occurrence Handle1:CAS:528:DyaK28XjsFKjt7w%3D Occurrence Handle8676383

    CAS  PubMed  Google Scholar 

  • Cronan JE & Rock CO (1996) In Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, and Umbarger HE (Ed) Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed pp 612–636 American Society for Microbiology, Washington, DC.

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433, People’s Republic of China

    Liu Wang, Chaoneng Ji, Yiren Xu, Jian Xu, Jianfeng Dai, Qihan Wu, Maoqing Wu, Xianqiong Zou, Liyun Sun, Shaohua Gu, Yi Xie & Yumin Mao

Authors
  1. Liu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chaoneng Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yiren Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianfeng Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qihan Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Maoqing Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xianqiong Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Liyun Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Shaohua Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Yi Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Yumin Mao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yumin Mao.

Additional information

The nucleotide sequence reported in this paper has been submitted to GenBank under accession number AY314787.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, L., Ji, C., Xu, Y. et al. Cloning and characterization of a novel human homolog* of mouse U26, a putative PQQ-dependent AAS dehydrogenase. Mol Biol Rep 32, 47–53 (2005). https://doi.org/10.1007/s11033-003-2716-4

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-003-2716-4

Keywords

Search

Navigation