Abstract
Trichomes are differentiated epidermal cells and can be found on above ground organs of nearly all land plants. Results from previous studies show that trichomes play important roles against a wide range of both biotic and abiotic stresses. By examining differences between parental genotypes of available populations, we identified a population of recombinant inbred lines showing clear segregation for trichome density and length. Assessing the F8 lines of the population growing in the field detected a major locus on chromosome arm 4BL. This locus was detected based the assessments of either fully expanded third leaves or flag leaves after anthesis. Based on the position of the QTL, an SSR marker was used to identify heterozygous plants at this locus from F5 lines derived from the same cross for the F8 population. Three pairs of near isogenic lines targeting this locus were obtained from these heterozygous plants. Difference in trichome length between the two lines with opposite alleles for each of these NIL pairs were similar to that between the two parental genotypes for the mapping populations, confirming that this single locus is mainly responsible for the trichome characteristics measured in this study. The allele with long and dense trichome is dominant as this characteristic was shown by the heterozygous individuals at this marker locus. Apart from the targeted locus, NIL pairs have highly homogeneous genetic backgrounds. Thus, the NILs could be invaluable in understanding the relationship between trichome density and resistance or tolerance to various biotic and abiotic stresses.
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Acknowledgements
Work reported here was supported by the Innovation Research Team Building Project of Agricultural Ecology from Fujian Academy of Agricultural Sciences (Project code: STIT2017-1-9) and the Commonwealth Scientific and Industrial Organization (CSIRO), Australia (Project code: R-04818). ZTC is grateful to the Young Talent Program of Fujian Academy of Agricultural Sciences (YC2016-7) for funding his visit to CSIRO Agriculture & Food in Australia. WL and HZ are grateful to the Sichuan Agricultural University and the China Scholarship Council for supports during the tenures of their PhD candidature. The authors are also grateful to Caritta Eliasson for her invaluable technical assistance and the two anonymous reviewers for their help in improving the manuscript.
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This study was funded by the Commonwealth Scientific and Industrial Organization (CSIRO), Australia (Project code: R-10191-01), the Young Talent Program of Fujian Academy of Agricultural Sciences (YC2016–7).
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ZTC, ZYY and CJL conceived the study; ZTC, ZZ, WL and HZ conducted experiments and analysed data; ZTC and CJL prepared the manuscript with contributions from all other authors.
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Chen, Z., Zheng, Z., Luo, W. et al. Detection of a major QTL conditioning trichome length and density on chromosome arm 4BL and development of near isogenic lines targeting this locus in bread wheat. Mol Breeding 41, 10 (2021). https://doi.org/10.1007/s11032-021-01201-8
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DOI: https://doi.org/10.1007/s11032-021-01201-8