Abstract
We investigated the functional properties of two 1Sl-encoded high molecular weight glutenin subunits (HMW-GS), 1Slx2.3* and 1Sly16*, from Aegilops longissima L., and developed single-nucleotide polymorphism (SNP)-based molecular markers. Two HMW-GS near-isogenic lines (NILs), HMW-NIL1 (1Ax1, 1Slx2.3* + 1Sly16*, and 1Dx2 + 1Dy12) and HMW-NIL2 (1Ax1, 1Bx17 + 1By18, and 1Dx2 + 1Dy12), were developed by crossing the ‘Chinese Spring’ (CS)-Ae. longissima 1Sl/1B substitution line (CS-1Sl/1B) with the spring wheat cultivar CB037A, and consecutive selfing combined with selection and identification. Quality analysis of the two NILs showed that the 1Slx2.3* + 1Sly16* subunits conferred better dough rheological properties and breadmaking quality than the 1Bx17 + 1By18 subunits; in particular, they significantly improved dough strength, loaf volume and texture, and final bread score. Allele-specific polymerase chain reaction markers for both genes were developed based on SNP variations and successfully amplified 290-bp and 283-bp specific fragments from the 1Slx2.3* and 1Sly16* genes, respectively. Both SNP-based molecular markers were further validated using 38 wheat varieties, two recombination inbred lines, and four F2 generations and showed high specificity and accuracy. Thus, these markers can be used for molecular marker-assisted selection in the early generations of wheat quality improvement programs.
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Abbreviations
- AS-PCR:
-
allelic-specific polymerase chain reaction
- HMW-GS:
-
High molecular weight glutenin subunits
- HPCE:
-
high-performance capillary electrophoresis
- LMW-GS:
-
Low molecular weight glutenin subunits
- MAS:
-
Marker-assisted selection
- MS:
-
mass spectrometry
- NILs:
-
near-isogenic lines
- RILs:
-
recombination inbred lines
- RP-HPLC:
-
reversed-phase high-performance liquid chromatography
- RP-UPLC:
-
reversed-phase ultra-performance liquid chromatography
- RVA:
-
Rapid Visco Analyzer
- SDS-PAGE:
-
Sodium dodecyl sulfate polyacrylamide gel electrophoresis
- SNPs:
-
Single-nucleotide polymorphisms
References
Ahmad M (2000) Molecular marker-assisted selection of HMW glutenin alleles related to wheat bread quality by PCR-generated DNA markers. Theor Appl Genet 101:892–896
Alberto C, Renato D, Antonio TO, Carla C, Marina P, Enrico P (2003) Genetic analysis of the Aegilops longissima, 3S chromosome carrying the Pm13, resistance gene. Euphytica 130:177–183
Altpeter F, Vasil V, Srivastava V, Vasil IK (1996) Integration and expression of the high molecular weight glutenin subunit 1Ax1 gene into wheat. Nat Biotechnol 14:1155–1199
Belton PS (1999) On the elasticity of wheat gluten. J Cereal Sci 29:103–107
Burnouf T, Bouriquet R (1980) Glutenin subunits of genetically related European hexaploid wheat cultivars: their relationships with breadmaking quality. Theor Appl Genet 58:107–111
Cenci A, D’Ovidio R, Tanzarella OA, Ceoloni C, Porceddu E (1999) Identification of molecular markers linked to Pm13, an Aegilops longissima gene conferring resistance to powdery mildew in wheat. Theor Appl Genet 98:448–454
Ceoloni C, Signore GD, Ercoli L, Donini P (1992) Locating the alien chromatin segment in common wheat Aegilops longissima mildew resistant transfers. Hereditas 116:239–245
D’Ovidio R, Anderson O (1994) PCR analysis to distinguish between alleles of a member of a multigene family correlated with wheat bread making quality. Theor Appl Genet 88:759–763
Gao LY, Ma WJ, Chen J, Wang K, Li J, Wang SL, Bekes F, Appels R, Yan YM (2010) Characterization and comparative analysis of wheat high molecular weight glutenin subunits by SDS-PAGE, RP-HPLC, HPCE, and MALDITOF-MS. J Agric Food Chem 58:2777–2786
Garg M, Kumar R, Singh RP, Tsujimoto H (2014) Development of an Aegilops longissima, substitution line with improved bread-making quality. J Cereal Sci 60:389–396
Gianibelli MC, Larroque OR, Macritchie F, Wrigley CW (2001) Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chemistry 78:635–646
Guo HY, Wu JS, Lu YX, Yan YM (2019) High molecular weight glutenin 1Bx17 and 1By18 subunits encoded by Glu-B1i enhance wheat dough rheological properties and breadmaking quality. Journal of Food Quality ID1958747
Halford NG, Forde J, Anderson OD, Greene FC, Shewry PR (1987) The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1b of bread wheat (Triticum aestivum L.) and comparison with those of genes from chromosomes 1A and 1D. Theor Appl Genet 75:117–126
Hassani ME, Gianibelli MC, Shariflou MR, Sharp PJ (2005) Molecular structure of a novel y-type HMW glutenin subunit gene present in Triticum tauschii. Euphytica 141:191–198
Hu JX, Jian W, Deng X, Yan YM (2019) Cloning and characterization of special HMW glutenin subunit genes from Aegilops longissima L. and their potential for wheat quality improvement. 3 Biotech 9:267
Jiang CX, Pei YH, Zhang YZ, Li XH, Yao DN, Yan YM, Ma WJ, Hsam SLK, Zeller FJ (2008) Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T zhukovskyi. Hereditas 145:92–98
Jiang PH, Xue JS, Duan LN, Gu YS, Mu JY, Han SC, Chen L, Li YX, Ma WJ, Yan YM, Li XH (2018) Effects of high-molecular-weight glutenin subunit combination in common wheat on the quality of crumb structure. J Sci Food Agric 99:1501–1508
Jongh G (1953) The baking value of wheat varieties grown in the Netherlands. Euphytica 2:6–14
Kari MT, Ellen MM, Pernille B (2003) Comparison of small and large deformation rheologic properties of wheat dough and gluten. Cereal Chemistry 80:587–595
Li XH, Ma WJ, Gao LY, Zhang YZ, Wang AL, Ji KM, Wang K, Appels R, Yan YM (2008) A novel chimeric low-molecular-weight glutenin subunit gene from the wild relatives of wheat Aegilops kotschyi and Ae. juvenalis: evolution at the Glu-3 loci. Genetics 180:93–101
Liang XN, Zhen SM, Han CX, Wang C, Li XH, Ma WJ, Yan YM (2015) Molecular characterization and marker development for hexaploid wheat-specific HMW glutenin subunit 1By18 gene. Mol Breed 35:221
Liu YL, Tian JC, Han XM, Deng ZY (2004) Comparison of different dough rheological measurement and path coefficient analysis of bread quality. Agicultural Sciences in China 3:468–474
Ma W, Appels R, Bekes F, Larroque O, Morell MK, Gale KR (2005) Genetic characterisation of dough rheological properties in a wheat doubled haploid population: additive genetic effects and epistatic interactions. Theor Appl Genet 111:410–422
Ma DY, Zhang Y, Xia XC, Morris MCF, He ZH (2010) Wheat flour, pan bread, and steamed bread qualities of common wheat near-isogenic lines differing in puroindoline b alleles: wheat flour, pan bread, and steamed bread qualities of common wheat near-isogenic lines differing in puroindoline b alleles. Acta Agron Sin 36:261–266
Mackie AM, Sharp PJ, Lagudah ES (1996) The nucleotide and derived amino acid sequence of a HMW glutenin gene from Triticum tauschii and comparison with those from the D genome of bread wheat. J Cereal Sci 24:73–78
Masci S, D’Ovidio R, Lafiandra D, Kasarda DD (1998) Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer. Plant Physiol 118:1147–1158
Masci S, D’Ovidio R, Lafiandra D, Kasarda DD (2000) A 1B coded low-molecular-weight glutenin subunit associated with quality in durum wheats show strong similarity to subunits present in some bread wheat cultivars. Theor Appl Genet 100:396–400
Neelam K, Rawat N, Tiwari VK, Ghandhi N, Arun PC, Kumar S, Tripathi SK, Randhawa GS, Prasad R, Dhaliwal HS (2013) Development and molecular characterization of wheat-Aegilops longissima derivatives with high grain micronutrients. Aust J Crop Sci 7:508–514
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35
Payne PI, Holt LM, Krattiger AF, Carrillo JM (1988) Relationships between seed quality characteristics and HMW glutenin subunit composition determined using wheats grown in Spain. J Cereal Sci 7:229–235
Pfeifer M, Kugler KG, Sandve SR, Zhan BJ, Rudi H, Hvidsten TR, Mayer KFX, Olsen O (2014) Genome interplay in the grain transcriptome of hexaploid bread wheat. Science 345:1250091
Qiao YQ, Ma CX, Huang ZL, Si HQ, Cai H, Xia YX (2008) Stability analysis on rva and relationship with major quality traits in common wheat. Acta Laser Biology Sinica 17:587–592
Rafalski A (2002) Applications of single nucleotide polymorphisms in crop genetics. Curr Opin Plant Biol 5:94–100
Rasheed A, Xia XC, Yan YM, Appels R, Mahmood T, He ZH (2014) Wheat seed storage proteins: advances in molecular genetics, diversity and breeding applications. J Cereal Sci 60:11–24
Sheng HY, Murray TD (2013) Identifying new sources of resistance to eyespot of wheat in Aegilops longissima. Plant Disease 97:346–353
Sheng HY, See DR, Murray TD (2012) Mapping QTL for resistance to eyespot of wheat in Aegilops longissima. Theor Appl Genet 125:355–366
Shewry PR, Halford NG, Tatham AS (1992) High molecular weight subunits of wheat glutenin. J Cereal Sci 15:105–120
Shiri Y, Solouki M, Forootan M (2015) Analysis of allelic variation in hmw-glu-1 gene blocks in iranian wheat cultivars using alp molecular marker. Cercetari Agronomice in Moldova 48:51–60
Sun H, Yan SP, Jiang WL, Li GT, MacRitchie F (2010) Contribution of lipid to physicochemical properties and Mantou-making quality of wheat flour. Food Chem 121:332–337
Tatham AS, Miflin BJ, Shewry PR (1985) The beta-turn conformation in wheat gluten proteins: relationship to gluten elasticity. Cereal Chem 62:405–412
Tilman D, Cassman KG, Matson PA, Naylor R, Polasky S (2002) Agricultural sustainability and intensive production practices. Nature 418:671–677
Wang MF (2013) The relationship between protein quality and starch pasting parameters and bread baking quality in common wheat. Journal of Nuclear Agricultural Sciences 27:792–799
Wang R, Li SB, Zhao CP, Wang GR, Pene RJ, Jorge ZH (1995) The relation between more spikelet and hige molecular weight subunit compositions in hexaploid wheat. Acta Botanica Boerall-Occidentalla Sinica 15:265–269
Wang GR, Zhou GY, Wang R (1997) The correlation between baking quality and dough development time and stability time. Journal of the Chinese Cereal and Oil Association 12:1–6
Wang SL, Yu ZT, Cao M, Shen XX, Li N, Li XH, Ma WJ, Weißgerber H, Zeller F, Hsam S, Yan YM (2013) Molecular mechanisms of HMW glutenin subunits from 1Sl genome of Aegilops longissima positively affecting wheat breadmaking quality. PLoS One 8:e58947
Wang C, Shen XX, Wang K, Liu YL, Zhou JW, Hu YH, Zeller FJ, Hsam SLK, Yan YM (2016) Molecular characterization and functional properties of two novel x-type HMW-GS from wheat line CNU608 derived from Chinese spring × Ae. caudata cross. J Cereal Sci 68:16–24
Wang J, Wang C, Zhen SM, Li XH, Yan YM (2017) Low molecular weight glutenin subunits from the 1U genome of Aegilops umbellulata confer superior dough rheological properties and improve breadmaking quality of bread wheat. J Sci Food Agric 98:2156–2167
Wang W, Wang K, Chen X, Prodanovic S, Li XH, Ye XG, Yan YM (2018) Molecular characterization and phylogenetic analysis of one Omega-gliadin gene from Aegilops speltoides L. Genetika 50:503–517
Weegels PL, Pijpekamp AMVD, Graveland A, Hamer RJ, Schofield JD (1996) Depolymerisation and re-polymerisation of wheat glutenin during dough processing and relationships between glutenin macropolymer content and quality parameters. J Cereal Sci 23:103–111
Wu JS, Lu XB, Yu ZT, Han CX, Li XH, Prodanovic S, Yan YM (2017) Effects of Glu-1 and Glu-3 allelic variations on wheat glutenin macropolymer (GMP) content as revealed by size-exclusion high performance liquid chromatography (SE-HPLC). Genetika 49:677–691
Xie RL, Wan YF, Zhang WY, Wang DW (2001) HMW glutenin subunits in multiploid Aegilops species: composition analysis and molecular cloning of coding sequences. Chinese Science Bulletin 4:309–313
Yan YM, Hsam SLK, Yu JZ, Jiang Y, Zeller FJ (2003a) Allelic variation of the HMW glutenin subunits in Aegilops tauschii accessions detected by sodium dodecyl sulphate (SDS-PAGE), acid polyacrylamide gel (A-PAGE) and capillary electrophoresis. Euphytica 130:377–385
Yan YM, Yu JZ, Jiang Y, Hu YK, Cai MH, Hsam SLK, Zeller FJ (2003b) Capillary electrophoresis separation of high molecular weight glutenin subunits in bread wheat (Triticum aestivum L.) and related species with phosphate-based buffers. Electrophoresis 24:1429–1436
Yan Y, Zheng J, Xiao Y, Yu J, Hu Y, Cai M, Li Y, Hsam SLK, Zeller FJ (2004) Identification and molecular characterization of a novel y-type Glu-D t 1 glutenin gene of Aegilops tauschii. Theor Appl Genet 108:1349–1358
Yan X, Liu W, Yu ZT, Han CX, Zeller FJ, Hsam SLK, Yan YM (2014) Rapid separation and identification of wheat HMW glutenin subunits by UPLC and comparative analysis with HPLC. Aust J Crop Sci 8:140–147
Zhang YZ, Li X, Yan YM, Xiong X, An XL, Zhang Q, Pei YH, Gao LY, Hsam SLK, Zeller FJ (2008a) Molecular characterization of two novel x-type HMW glutenin genes from Aegilops tauschii and implications for the evolution of Glu-D1-1 alleles. Genetics 178:23–33
Zhang Q, Dong YM, An XL, Wang AL, Zhang YZ, Li XH, Gao LY, Xia XC, He ZH, Yan YM (2008b) Characterization of HMW glutenin subunits in common wheat and related species by matrix-assisted laser desorption/ionization time of-flight mass spectrometry (MALDI-TOF-MS). J Cereal Sci 47:252–261
Zhao H, Lu SY, Li ZZ (1994) Studies on inheritance and variation of hmw glutenin subunits and their correlation with quality and other agronomic characters in wheat. Acta Agronomica Sinica 20:67–75
Zhen SM, Zhou JX, Deng X, Zhu GR, Cao H, Wang ZM, Yan YM (2016) Metabolite profiling of the response to high-nitrogen fertilizer during grain development of bread wheat (Triticum aestivum L.). J Cereal Sci 69:85–94
Zhou JX, Ma CY, Zhen SM, Cao M, Zeller FJ, Hsam SLK, Yan YM (2016) Identification of drought stress related proteins from 1S1(1B) chromosome substitution line of wheat variety Chinese Spring. Bot Stud 57:2–10
Zhu GR, Wang SL, Zhen SM, Shen XX, Prodanovic S, Yan YM (2015) Molecular characterization and phylogenetic analysis of unusual x-type HMW glutenin subunits from 1Sl genome of Aegilops longissima. Genetika 47:185–203
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This research was financially supported by grants from National Key R. & D. Program of China (2016YFD0100502) and the National Natural Science Foundation of China (31771773).
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Cui, D., Wang, J., Li, M. et al. Functional assessment and SNP-based molecular marker development of two 1Sl-encoded HMW glutenin subunits in Aegilops longissima L.. Mol Breeding 39, 120 (2019). https://doi.org/10.1007/s11032-019-1025-0
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DOI: https://doi.org/10.1007/s11032-019-1025-0