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Functional assessment and SNP-based molecular marker development of two 1Sl-encoded HMW glutenin subunits in Aegilops longissima L.

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Abstract

We investigated the functional properties of two 1Sl-encoded high molecular weight glutenin subunits (HMW-GS), 1Slx2.3* and 1Sly16*, from Aegilops longissima L., and developed single-nucleotide polymorphism (SNP)-based molecular markers. Two HMW-GS near-isogenic lines (NILs), HMW-NIL1 (1Ax1, 1Slx2.3* + 1Sly16*, and 1Dx2 + 1Dy12) and HMW-NIL2 (1Ax1, 1Bx17 + 1By18, and 1Dx2 + 1Dy12), were developed by crossing the ‘Chinese Spring’ (CS)-Ae. longissima 1Sl/1B substitution line (CS-1Sl/1B) with the spring wheat cultivar CB037A, and consecutive selfing combined with selection and identification. Quality analysis of the two NILs showed that the 1Slx2.3* + 1Sly16* subunits conferred better dough rheological properties and breadmaking quality than the 1Bx17 + 1By18 subunits; in particular, they significantly improved dough strength, loaf volume and texture, and final bread score. Allele-specific polymerase chain reaction markers for both genes were developed based on SNP variations and successfully amplified 290-bp and 283-bp specific fragments from the 1Slx2.3* and 1Sly16* genes, respectively. Both SNP-based molecular markers were further validated using 38 wheat varieties, two recombination inbred lines, and four F2 generations and showed high specificity and accuracy. Thus, these markers can be used for molecular marker-assisted selection in the early generations of wheat quality improvement programs.

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Abbreviations

AS-PCR:

allelic-specific polymerase chain reaction

HMW-GS:

High molecular weight glutenin subunits

HPCE:

high-performance capillary electrophoresis

LMW-GS:

Low molecular weight glutenin subunits

MAS:

Marker-assisted selection

MS:

mass spectrometry

NILs:

near-isogenic lines

RILs:

recombination inbred lines

RP-HPLC:

reversed-phase high-performance liquid chromatography

RP-UPLC:

reversed-phase ultra-performance liquid chromatography

RVA:

Rapid Visco Analyzer

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SNPs:

Single-nucleotide polymorphisms

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The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/9VVQDl.

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This research was financially supported by grants from National Key R. & D. Program of China (2016YFD0100502) and the National Natural Science Foundation of China (31771773).

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Correspondence to Yueming Yan.

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Fig. S1

Molecular marker primers of 1Slx2.3* gene. The red box indicates the primer 1Slx2.3*F and 1Slx2.3*R. (DOCX 639 kb).

Fig. S2

Molecular marker primers of 1Sly16* gene. The red box indicates the primer 1Sly16*F and 1Sly16*R. (DOCX 390 kb).

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Cui, D., Wang, J., Li, M. et al. Functional assessment and SNP-based molecular marker development of two 1Sl-encoded HMW glutenin subunits in Aegilops longissima L.. Mol Breeding 39, 120 (2019). https://doi.org/10.1007/s11032-019-1025-0

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