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Identification of main effect and epistatic quantitative trait loci for morphological and yield-related traits in peanut (Arachis hypogaea L.)

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Abstract

An effort was made in the present study to identify the main effect and epistatic quantitative trait locus (QTL) for the morphological and yield-related traits in peanut. A recombinant inbred line (RIL) population derived from TAG 24 × GPBD 4 was phenotyped in seven environments at two locations. QTL analysis with available genetic map identified 62 main-effect QTLs (M-QTLs) for ten morphological and yield-related traits with the phenotypic variance explained (PVE) of 3.84–15.06%. Six major QTLs (PVE > 10%) were detected for PLHT, PPP, YPP, and SLNG. Stable M-QTLs appearing in at least two environments were detected for PLHT, LLN, YPP, YKGH, and HSW. Five M-QTLs governed two traits each, and 16 genomic regions showed co-localization of two to four M-QTLs. Intriguingly, a major QTL reported to be linked to rust resistance showed pleiotropic effect for yield-attributing traits like YPP (15.06%, PVE) and SLNG (13.40%, PVE). Of the 24 epistatic interactions identified across the traits, five interactions involved six M-QTLs. Three interactions were additive × additive and remaining two involved QTL × environment (QE) interactions. Only one major M-QTL governing PLHT showed epistatic interaction. Overall, this study identified the major M-QTLs for the important productivity traits and also described the lack of epistatic interactions for majority of them so that they can be conveniently employed in peanut breeding.

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  • 01 June 2018

    The published online version of this article unfortunately missed to capture Rajeev K. Varshney as co-corresponding author. There should have been two corresponding authors for this paper (Rajeev K. Varshney and Ramesh S. Bhat). The correct declaration is shown below.

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Acknowledgements

This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. The authors would like to thank Erin Higgins for valuable comments to improve the quality of the manuscript.

Funding

The work presented in this article is a contribution from research projects sponsored by National Funds for Basic Strategic and Frontier Application Research in Agriculture (NFBSFARA) of Indian Council of Agricultural Research (ICAR), New Delhi, India, and World Bank assisted Karnataka Watershed Development Project-II (KWDP-II) funded by Government of Karnataka (GoK), India.

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Author notes

  1. Yogendra Khedikar and Manish K. Pandey contributed equally to this work.

Authors and Affiliations

  1. Department of Genetics and Plant Breeding, University of Agricultural Sciences, Dharwad, India

    Yogendra Khedikar, V. Sujay, Cholin Sarvamangala, Ganapati Mukri & M. V. C. Gowda

  2. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, India

    Yogendra Khedikar, Manish K. Pandey, V. Sujay, Sube Singh, Spurthi N. Nayak, Cholin Sarvamangala, Vanika Garg, Hari D. Upadhyaya & Rajeev K. Varshney

  3. Department of Biotechnology, University of Agricultural Sciences, Dharwad, India

    Spurthi N. Nayak, H. L. Nadaf & Ramesh S. Bhat

  4. Agriculture and Agri-Food Canada, Beaverlodge Research Farm, Beaverlodge, Alberta, Canada

    Henry W. Klein-Gebbinck

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  1. Yogendra Khedikar
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  2. Manish K. Pandey
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Electronic supplementary material

Supplementary Figure S1

Heatmap of Pearson’s correlation coefficients (r) for all the traits in each environment. The significant correlations are color-coded. (PDF 154 kb)

Supplementary Figure S2

Major effect QTLs detected for agro-morphological traits among the RILs of peanut. (PDF 101 kb)

Supplementary Figure S3

QTL cartographer plot showing co-mapped QTLs for different agro-morphological traits among the RILs of peanut. (PDF 157 kb)

Supplementary Table S1

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Supplementary Table S2

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Supplementary Table S3

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Khedikar, Y., Pandey, M.K., Sujay, V. et al. Identification of main effect and epistatic quantitative trait loci for morphological and yield-related traits in peanut (Arachis hypogaea L.). Mol Breeding 38, 7 (2018). https://doi.org/10.1007/s11032-017-0764-z

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