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Single nucleotide polymorphism tightly linked to a major QTL on chromosome 7A for both kernel length and kernel weight in wheat

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Abstract

Thousand-kernel weight (TKW) is one of the major components of grain yield in wheat (Triticum aestivum). Identifying major quantitative trait loci (QTLs) for TKW and developing effective markers are prerequisite for success in marker-assisted selection (MAS) to improve wheat yield through breeding. This study mapped a major QTL, designated as TaTKW-7AL, for increasing TKW on the long arm of chromosome 7A of ‘Clark’ to a 1.3-cM interval between single nucleotide polymorphism (SNP) markers IWB13913 and IWA5913. This QTL explained 19.7 % of the phenotypic variation for TKW. A QTL for increasing kernel length (KL), one of the major components of TKW, was mapped in the same interval as TaTKW-7AL, suggesting that increased TKW by the QTL in ‘Clark’ is most likely due to the increased KL. Association analysis on a diversity panel of 200 US winter wheat accessions also identified a haplotype of three SNP markers (IWB13913, IWB6693 and IWA5913) that were tightly associated with the both KL and TKW. The analysis of allele frequencies of the haplotype in the diversity panel suggested that the favorable allele of TaTKW-7AL has not been strongly selected for in practice and has potential to be used to improve grain yield in US hard winter wheat breeding. Two user-friendly flanking KASPar markers, IWB13913 and IWA5913, were developed for MAS of TaTKW-7AL.

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Acknowledgments

The authors thank Dr. Brett Carver from Oklahoma State University, Stillwater, OK for providing part of wheat breeding lines used in this study and help with the Enid field experiment. This project is partly funded by National Natural Science Foundation of China (31401381), China, and the National Research Initiative Competitive Grants CAP Project 2011-68002-30029 from the USDA National Institute of Food and Agriculture, USA. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang, 050035, China

    Zhenqi Su

  2. Department of Agronomy, Kansas State University, Manhattan, KS, 66506, USA

    Zhenqi Su, Sujuan Jin, Yue Lu, Guorong Zhang & Guihua Bai

  3. Shijiazhuang Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050041, China

    Sujuan Jin

  4. USDA Cereal Crops Research Unit, Fargo, ND, 58102, USA

    Shiaoman Chao

  5. USDA Hard Winter Wheat Genetics Research Unit, Manhattan, KS, 66506, USA

    Guihua Bai

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  1. Zhenqi Su
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Correspondence to Guihua Bai.

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Su, Z., Jin, S., Lu, Y. et al. Single nucleotide polymorphism tightly linked to a major QTL on chromosome 7A for both kernel length and kernel weight in wheat. Mol Breeding 36, 15 (2016). https://doi.org/10.1007/s11032-016-0436-4

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