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The glutathione synthesis may be regulated by cadmium-induced endogenous ethylene in Lycium chinense, and overexpression of an ethylene responsive transcription factor gene enhances tolerance to cadmium stress in tobacco

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Abstract

Glutathione (GSH) plays a pivotal role in heavy metal detoxification. Ethylene is one of the important plant hormones, which plays a critical role in triggering plant responses to different stresses such as cadmium (Cd) stress. Ethylene responsive transcription factor (ERF) belongs to one of the largest plant transcription factor families. ERF is known to be induced by ethylene and thus regulate multiple stress responses through the activation of stress-related genes. Until now, little has been done to explore the relationship among the accumulation of endogenous ethylene, ERF transcript levels and the GSH content in plants under Cd treatment and we will investigate these link. In this study, the gene transcript level of LchERF, LchGSH1 (gene responsible for the first-step GSH biosynthesis) and LchGSHS (gene responsible for the second-step GSH biosynthesis), endogenous ethylene accumulation, GSH content and Cd concentration in Lycium chinense with or without Cd stress treatment were studied. Furthermore, the transgenic tobacco expressing 35S::LchERF which grown under Cd stress condition was also investigated in this study. Our results showed that endogenous ethylene, LchERF, LchGSH1 and LchGSHS gene expression and GSH content can be induced by Cd treatment in L. chinense, however, reduced by co-treatment with 2-aminoethoxyvinlglycine (AVG), an inhibitor of ethylene biosynthesis. The transgenic tobacco expressing 35S::LchERF showed greater tolerance to Cd stress than non-transgenic plants. The expression of NtGSH1 and NtGSHS genes was increased in transgenic tobacco plants compared with non-transgenic plants, indicating that LchERF is associated with the expression level of GSH synthesis related genes in tobacco. Evidence was presented here that under Cd stress, GSH accumulation occurred at least partially via enhanced LchERF gene expression and the ethylene signal transduction pathways might be involved in this accumulation.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31401391), Tianjin Research Program of Application Foundation and Advanced Technology (No.15JCQNJC14700).

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, People’s Republic of China

    Chunfeng Guan, Jing Ji, Chao Jin & Gang Wang

  2. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Dianyun Wu & Wenzhu Guan

  3. Department of Medical Genetics, Tianjin Medical University General Hospital, Tianjin, 300072, People’s Republic of China

    Xiaozhou Li

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  1. Chunfeng Guan
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  2. Jing Ji
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  3. Dianyun Wu
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  4. Xiaozhou Li
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Correspondence to Jing Ji or Gang Wang.

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Guan, C., Ji, J., Wu, D. et al. The glutathione synthesis may be regulated by cadmium-induced endogenous ethylene in Lycium chinense, and overexpression of an ethylene responsive transcription factor gene enhances tolerance to cadmium stress in tobacco. Mol Breeding 35, 123 (2015). https://doi.org/10.1007/s11032-015-0313-6

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