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Identification of quantitative trait loci for cane splitting in red raspberry (Rubus idaeus)

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Abstract

Cane splitting, a normal feature of raspberry growth, can lead to plant infestation by cane midge followed by fungal infection, with losses in yield of up to 50 % if left untreated. The extent of splitting in the Latham × Glen Moy reference mapping population was assessed over six years and in three environments and quantitative trait loci (QTL) were identified across linkage groups (LG) 2, 3, 5 and 6. Cane splitting QTL on LG 3 and 5 co-locate with QTL for plant vigour. The cane splitting QTL on LG 6 is associated with the QTL for resistance to root rot caused by Phytophthora rubi. Broad-sense heritability for cane splitting ranged from 25.6 % in 2007 to 49.1 % in 2008 in this population. Season and environment were also found to influence cane splitting in this population. Several genes involved in general plant growth and in defence responses lie within these QTL. This is a first step towards identifying the genetic basis of cane splitting in raspberry and the development of genetic markers for use in raspberry breeding programmes.

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Abbreviations

BAC:

Bacterial artificial chromosome

PRR:

Phytophthora root rot

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Acknowledgments

We thank Clare Booth and Louise Donnelly at the James Hutton Institute (JHI) Sequencing and Genotyping Service and Joanne Russell, Luke Ramsay (JHI) and Janet Allen of ADAS for helpful comments. This work is funded by the Scottish Government Rural and Environment Research and Analysis Directorate (UK).

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Authors and Affiliations

  1. Department of Genetics, James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK

    Mary Woodhead, Sandie Williamson, Kay Smith, Susan McCallum, Nikki Jennings & Julie Graham

  2. Biomathematics and Statistics Scotland, Invergowrie, Dundee, DD2 5DA, UK

    Christine Hackett

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  1. Mary Woodhead
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  2. Sandie Williamson
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  4. Susan McCallum
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  6. Christine Hackett
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  7. Julie Graham
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Correspondence to Mary Woodhead.

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Supplementary material 1 (DOCX 18 kb)

11032_2012_9775_MOESM2_ESM.bmp

Supplementary Fig. 1 Correlation between raspberry cane height and cane splitting in 2005 in the Latham × Glen Moy raspberry reference mapping population (BMP 224 kb)

11032_2012_9775_MOESM3_ESM.bmp

Supplementary Fig. 2 Plot of the first principal coordinate scores calculated from the cane splitting (2005–2008) and root rot scores (2005–2006) from the 188 progeny from the Latham × Glen Moy raspberry reference mapping population. High principal coordinate scores correspond to high cane splitting or root rot scores (BMP 224 kb)

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Woodhead, M., Williamson, S., Smith, K. et al. Identification of quantitative trait loci for cane splitting in red raspberry (Rubus idaeus). Mol Breeding 31, 111–122 (2013). https://doi.org/10.1007/s11032-012-9775-y

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