Abstract
The tomato yellow leaf curl virus (TYLCV), transmitted by whitefly, causes major disease losses to tomato crops in tropical and subtropical regions of the world. Several genes conferring resistance to TYLCV, mainly Ty-1 and Ty-3 genes, have been introgressed to cultivated tomato (Solanum lycopersicum) from the wild relative species Solanum chilense. By combining bulked segregant analysis and amplified fragment length polymorphisms (AFLP), several AFLP markers closely linked to Ty-1 and Ty-3 genes were identified from the resistant breeding line TZ841-4. Cloning and sequencing of the selected AFLP fragments allowed us to develop codominant cleaved amplified polymorphic sequence and dominant sequence characterized amplified region markers closely linked to Ty-1. In addition, Ty-3-linked allelic-specific markers have been discriminated by a quantitative real-time PCR protocol. Taken together, these markers constitute useful tools for marker-assisted selection breeding programs to improve genetic resistance to TYLCV, and also to initiate map-based cloning approaches to isolate the resistance genes.
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Acknowledgments
We thank Fernando J. Yuste-Lisbona for critical reading of the manuscript and Eduardo R. Bejarano for providing the infectious clone of TYLCV-IL. This work was supported by grants from Ministerio de Ciencia e Innovación (AGL2007-66062-C02-01 and 05-0004) and Junta de Andalucía (IDEA 130278D). We also thank Campus de Excelencia Internacional Agroalimentario, CeiA3 for providing financial support.
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González-Cabezuelo, J.M., Capel, J., Abad, J. et al. Genotyping selection for resistance against tomato yellow leaf curl virus (TYLCV) conferred by Ty-1 and Ty-3 genes in tomato. Mol Breeding 30, 1131–1142 (2012). https://doi.org/10.1007/s11032-012-9701-3
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DOI: https://doi.org/10.1007/s11032-012-9701-3