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Peripheral glucose metabolism is altered by epileptic seizures

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Abstract

The aim of the present study was to investigate the status of jejunal absorption and peripheral metabolism of glucose in Wistar Audiogenic Rats (WAR), a genetic model of epilepsy, after seizures induced by intensive sound exposure. The jejunal loop of rats was isolated and infused (0.5 mL min−1) with Tyrode solution containing twice the normal concentrations of glucose, sodium, and potassium. Samples were taken at 5 or 10-min intervals over a 40-min period. At the end of the experiment, samples of liver and gastrocnemius muscle were taken to measure the levels of glycogen, glucose-6-phosphate, fructose-6-phosphate and glucose transporter-4 (GLUT4). Hepatic glucose-6-phosphate increased in WAR submitted to audiogenic seizure (21.90 ± 3.08) as compared to non-susceptible Wistar rats (8.12 ± 0.87) and to WAR not submitted to audiogenic stimulation (5.17 ± 0.97). In addition, an increase in hepatic fructose-6-phosphate, an intermediate metabolite of the glycolytic pathway, was observed in WAR submitted to audiogenic seizure (5.98 ± 0.99) compared to non-susceptible Wistar rats (2.38 ± 0.53). According to the present results, jejunal absorption of glucose was not changed by seizures. However, generalized tonic–clonic seizures produced by sound stimulation resulted in a decrease in muscle glycogen content. In addition, our results demonstrated that the concentration of GLUT4 in the gastrocnemius muscle of WAR was 1.6-fold higher than that observed in resistant rats and that the audiogenic stimulus led to decreased concentration of this receptor in the muscle of WAR animals.

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Acknowledgements

We would like to thank Dr. Cândido Celso Coimbra for helpful advice and Dr Frederic Jean Georges Frezard for English revision. Fernanda K. S. Pereira was the recipient of a CNPq fellowship.

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Authors and Affiliations

  1. Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-010, Belo Horizonte, Minas Gerais, Brazil

    Fernanda K. S. Pereira, Almir A. Braga, Jorge Luiz Pesquero, Maria Carolina Doretto & Elizabeth L. Borges

  2. Centro de Desenvolvimento da Tecnologia Nuclear (CDTN)/Comissão Nacional de Energia Nuclear (CNEN), Belo Horizonte, Minas Gerais, Brazil

    Maria José Neves

  3. Departamento de Enfermagem Básica, Escola de Enfermagem, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Mércia P. Lima

Authors
  1. Fernanda K. S. Pereira
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  2. Maria José Neves
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  3. Mércia P. Lima
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  4. Almir A. Braga
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  5. Jorge Luiz Pesquero
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  6. Maria Carolina Doretto
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  7. Elizabeth L. Borges
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Correspondence to Elizabeth L. Borges.

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Pereira, F.K.S., Neves, M.J., Lima, M.P. et al. Peripheral glucose metabolism is altered by epileptic seizures. Metab Brain Dis 23, 105–114 (2008). https://doi.org/10.1007/s11011-007-9075-0

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  • DOI: https://doi.org/10.1007/s11011-007-9075-0

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