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Characterization of exosomal long non-coding RNAs in chondrogenic differentiation of human adipose-derived stem cells

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Abstract

The exosomes derived from chondrogenic stem cells and long non-coding RNAs (lncRNAs) play a key role in cartilage regeneration. Here, we investigated the expression profile of exosomal lncRNAs in chondrogenesis of human adipose derived stem cells (hADSCs). hADSCs were induced to differentiate into chondrocytes in vitro. Exosomes from undifferentiated hADSCs and chondrogenic hADSCs were isolated. LncRNA and mRNA expression profiles in the isolated exosomes were analyzed by RNA sequencing. The resultant data were subjected to gene ontology (GO) terms and KEGG pathway analysis to identify differentially expressed lncRNAs. We identified 23 upregulated and 163 downregulated lncRNAs in exosomes derived from chondrogenic hADSCs compared to that in exosomes from undifferentiated hADSCs. In addition, analysis of mRNA expression data revealed 968 upregulated genes and 572 downregulated genes in exosomes of chondrogenic hADSCs. Lncrna and mRNA expression levels were further validated by qRT–PCR. Differentially expressed lncRNAs and mRNAs were utilized to construct a coding–non–coding gene co-expression network (CNC network). GO terms and KEGG pathway enrichment analysis revealed several significant processes differentially regulated between undifferentiated hADSCs and chondrogenic hADSCs. Taken together, this study revealed the differential expression of exosomal lncRNAs of chondrogenic hADSCs and provided a foundation for future study on the cartilage recovery mechanism of exosomes derived from chondrogenic stem cells.

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Acknowledgements

This study was supported by the National Nature Science Foundation of China (81874014, 81672198, 81802187), Natural Science Foundation of Guangdong Province (2018030310355, 2017A030313804).

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Author notes

  1. Ziji Zhang and Guangxin Huang have contributed equally to this study.

Authors and Affiliations

  1. Department of Joint Surgery, the First Affiliated Hospital of Sun Yat-Sen University, #58 zhongshan 2nd road, Guangzhou, 510080, China

    Ziji Zhang & Guping Mao

  2. Department of Joint Surgery, The Third Affiliated Hospital of Southern Medical University, #183 zhongshan road, Guangzhou, 510630, China

    Guangxin Huang & Shu Hu

Authors
  1. Ziji Zhang
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  2. Guangxin Huang
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  3. Guping Mao
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  4. Shu Hu
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Correspondence to Guping Mao or Shu Hu.

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This study adhered to the standards of the Ethics committee on Human Experimentation at the First Affiliated Hospital of Sun Yat-Sen University, China (IRB:2014C-028) and the Helsinki Declaration (2000). Informed consent was obtained from all participants.

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Zhang, Z., Huang, G., Mao, G. et al. Characterization of exosomal long non-coding RNAs in chondrogenic differentiation of human adipose-derived stem cells. Mol Cell Biochem 476, 1411–1420 (2021). https://doi.org/10.1007/s11010-020-04003-2

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  • DOI: https://doi.org/10.1007/s11010-020-04003-2

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