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Effect of reversine on cell cycle, apoptosis, and activation of hepatic stellate cells

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Abstract

Experimental and clinical evidence show that liver fibrosis is potentially reversible. Hepatic stellate cells (HSCs) play a key role in the development of liver fibrosis. Some studies have shown that reversine could induce cell apoptosis. We attempted to elucidate the effect of reversine on cell cycle, apoptosis, and activation of HSCs. Data showed that reversine induced morphological changes in HSCs, inhibited cell proliferation, and induced cell-cycle arrest at the G2/M phase. Reversine induced cell apoptosis through caspase-dependent and mitochondria-dependent pathways. Reversine inhibited the activation of HSCs through TGF-β signaling pathway and degraded extracellular matrix protein collagen-I. The decreased TIMP1 and TGF-β1 proteins promoted fibrosis reversion. Reversine might be a promising drug for liver fibrosis reversion because it induces HSCs apoptosis, restrains cell proliferation, reduces HSCs activation, and degrades extracellular matrix in vitro.

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Funding

This project was funded by Guangdong Science and Technology Foundation (NO 2011B061300024, 2013B021800057) and Guangdong National Science Foundation (NO 10151006001000013).

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Authors and Affiliations

  1. Department of Hepatobiliary Surgery, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510180, People’s Republic of China

    Yu Huang, Di Huang, Jiefeng Weng, Shuai Zhang, Qiang Zhang, Zhenhao Mai & Weili Gu

  2. Guangzhou Digestive Disease Center, Guangzhou First People’s Hospital, No.1 Panfu Road, Yuexiu District, Guangzhou, Guangdong, 510180, People’s Republic of China

    Di Huang, Jiefeng Weng, Shuai Zhang & Weili Gu

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  1. Yu Huang
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Correspondence to Weili Gu.

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Huang, Y., Huang, D., Weng, J. et al. Effect of reversine on cell cycle, apoptosis, and activation of hepatic stellate cells. Mol Cell Biochem 423, 9–20 (2016). https://doi.org/10.1007/s11010-016-2815-x

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  • DOI: https://doi.org/10.1007/s11010-016-2815-x

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