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Combined therapeutic efficacy of carvacrol and X-radiation against 1,2-dimethyl hydrazine-induced experimental rat colon carcinogenesis

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A Correction to this article was published on 30 November 2021

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Abstract

Colon cancer is one of the most commonly diagnosed cancers, and is a major cause of cancer morbidity and mortality worldwide. The objective of the present study is to evaluate the combined therapeutic efficacy of carvacrol (CVC) and X-radiation against 1,2-dimethylhydrazine-induced colon cancer. Male albino Wistar rats were randomly divided into six groups. Group 1 served as control; group 2 received 40 mg/kg b.wt of CVC orally everyday throughout the experimental period (32 weeks); groups 3–6 received subcutaneous injections of DMH (20 mg/kg b.wt), once a week for the first 15 weeks; group 4 received a single dose of X-radiation at the 31st week; group 5 received CVC (40 mg/kg b.wt) two days after the last injection of DMH and continued everyday till the end of the experimental period; group 6 received CVC as in group 5 and radiation as in group 4. DMH-treated rats showed increased incidence of aberrant crypt foci (ACF), dysplastic aberrant crypt foci (DACF), mast cell number, argyrophilic nucleolar organizer regions; elevated activities of phase I enzymes, decreased activities of phase II enzymes, decreased mucin content and altered colonic and liver histology as compared to control rats. Though the individual treatments with CVC and X-radiation to DMH-treated rats reversed the above changes, the combined treatment with both CVC and X-radiation showed a marked effect. Our findings emphasize the potential role of combined therapeutic effect of CVC and X-radiation against DMH-induced colon carcinogenesis.

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Acknowledgments

We gratefully acknowledge the management of Dr. Kamakshi Memorial hospital, Chennai, Tamil Nadu, India for having provided necessary radiation facilities to complete this study.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chennai, Tamil Nadu, 608 002, India

    Sivaranjani Arivalagan, Nisha Susan Thomas, Balaji Chandrasekaran, Vijay Mani, Aktarul Islam Siddique & Nalini Namasivayam

  2. Medical Physics Division, Dr. Kamakshi Memorial Hospital, No: 1, Radial Road, Pallikaranai, Chennai, Tamil Nadu, 600 100, India

    Thayalan Kuppsamy

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  1. Sivaranjani Arivalagan
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  2. Nisha Susan Thomas
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Arivalagan, S., Thomas, N.S., Chandrasekaran, B. et al. Combined therapeutic efficacy of carvacrol and X-radiation against 1,2-dimethyl hydrazine-induced experimental rat colon carcinogenesis. Mol Cell Biochem 410, 37–54 (2015). https://doi.org/10.1007/s11010-015-2536-6

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