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Cholesterol ester transfer protein and apolipoprotein E gene polymorphisms in hyperlipidemic Asian Indians in North India

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Abstract

We determined the distribution of the polymorphic variants of CETP TaqIB and ApoE genes and their association with lipid and anthropometric parameters in hyperlipidemic and normolipidemic Asian Indians in North India. CETP TaqIB and ApoE polymorphism were assayed by PCR–RFLP in hyperlipidemic (n = 220) and normolipidemic (n = 367) subjects. Plasma lipids levels were estimated using commercially available kits from Randox (USA). The distribution of CETP TaqIB genotypes and alleles did not differ between the two groups. The frequency of ApoE ε4 allele was significantly higher in hyperlipidemic than normolipidemic subjects. Serum lipid levels were comparable between subjects with the different CETP TaqIB and ApoE genotypes in the two groups. Multivariate analysis after adjusting for age, sex, BMI, WHR, and total skinfold thickness showed that subjects with the Ε3Ε4 genotype and ε4 allele carriers were at significantly higher odds to develop hyperlipidemia [2.07 (1.29–3.30) and 2.05 (1.30–3.24), respectively] as compared to the other genotypes. ApoE ε4 allele and E3E4 genotype emerged as important genetic markers for hyperlipidemia in this study population.

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Abbreviations

Apo:

Apolipoprotein

Arg:

Arginine

BMI:

Body mass index

CVD:

Cardiovascular disease

CE:

Cholesterol ester

CAD:

Coronary artery disease

CHD:

Coronary heart disease

CETP:

Cholesterol ester transfer protein

Cys:

Cysteine

dNTP:

Deoxynucleoside triphosphate

DMSO:

Dimethylsulphoxide

EDTA:

Ethylene diamine tetra acetic acid

HDL:

High density lipoprotein

HDL-c:

High density lipoprotein cholesterol

HC:

Hip circumference

IDL:

Intermediate density lipoprotein

LCAT:

Lecithin cholesterol acyltransferase

LDL:

Low density lipoprotein

LDL-c:

Low density lipoprotein cholesterol

LDL-R:

Low density lipoprotein-receptor

LRP:

LDL-related protein

MetS:

Metabolic syndrome

OR:

Odds ratio

PAGE:

Polyacrylamide gel electrophoresis

PCR:

Polymerase chain reaction

PPHL:

Postprandial hyperlipidemia

RCT:

Reverse cholesterol transport

RFLP:

Restriction fragment length polymorphism

SNP:

Single nucleotide polymorphism

SD:

Standard deviation

T2DM:

Type 2 diabetes mellitus

TEMED:

N,N,N′,N′-Tetramethyl ethylenediamine

TC:

Total cholesterol

TG:

Triglyceride

TRL:

Triglyceride-rich lipoprotein

TSF:

Total skinfold

VLDL:

Very low density lipoprotein

VLDL-c:

Very low density lipoprotein cholesterol

WHR:

Waist hip ratio

WC:

Waist circumference

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Acknowledgment

This work was funded by the Indian Council of Medical Research (Ref: No. 53/14/2003-BMS), New Delhi, India.

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Authors and Affiliations

  1. Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India

    Kiran Meena & Kalpana Luthra

  2. Diabetes Unit, Fortis Hospital, B-1, Vasant Kunj, New Delhi, India

    Anoop Misra

  3. Department of Medicine, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India

    Naval Vikram

  4. Department of Biochemistry, Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India

    Shakir Ali

  5. Department of Biostatistics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, 110029, India

    R. M. Pandey

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  1. Kiran Meena
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Correspondence to Kalpana Luthra.

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Meena, K., Misra, A., Vikram, N. et al. Cholesterol ester transfer protein and apolipoprotein E gene polymorphisms in hyperlipidemic Asian Indians in North India. Mol Cell Biochem 352, 189–196 (2011). https://doi.org/10.1007/s11010-011-0753-1

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