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Alterations in band 3 protein and anion exchange in red blood cells of renal failure patients

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Abstract

The precise nature of band 3 protein and its involvement in oxalate exchange in the red blood cells (RBCs) of renal failure patients has not been studied in detail. Therefore, here we studied the oxalate exchange and binding by band 3 protein in RBCs of humans with conditions of acute and chronic renal failure (ARF and CRF). The RBCs of ARF and CRF patients exhibited abnormal red cell morphology and an increased resistance to osmotic hemolysis. Further, an increase in the cholesterol content and decrease in the activities of Na+-K+-, Ca2+-, and Mg2+-ATPases of membranes were observed in the RBCs of ARF and CRF patients. A decrease in the oxalate flux was observed in the RBCs of ARF and CRF patients. The oxalate-binding activities of the RBC membranes were significantly lower in ARF (20 pmoles/mg protein) and CRF (5.3 pmoles/mg protein) patients as compared to that in the normal subjects (36 pmoles/mg protein). DEAE-cellulose and Sephadex G-200 column chromatography purification profiles revealed a distinctive shift in oxalate-binding activity of band 3 protein of RBCs of ARF and CRF patients as compared to that of the normal subjects. It was also observed from the binding studies with a fluorescent dye, eosin-5-maleimide, which specifically binds to band 3 protein, that the RBCs of ARF and CRF patients exhibited only 53 and 32% of abundance of band 3 protein, respectively, as compared to that in the RBCs of the normal subjects, thus revealing a decrease in the band 3 protein content in ARF and CRF patients. These results for the first time showed a decrease in the oxalate exchange in RBCs of patients with ARF and CRF, which was also concomitant with the low levels of abundance of band 3 protein.

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Authors and Affiliations

  1. Department of Internal Medicine, Lipomics and Lipid Signaling Laboratory, The Ohio State University, Columbus, OH, U.S.A.

    Varadharaj Saradhadevi & Narasimham Parinandi

  2. Department of Medical Biochemistry, Dr. A.L.M Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India

    Ramasamy Sakthivel & Ramasamy Selvam

  3. Department of Physiology and Cell Biology, The Ohio State University, Columbus, OH, U.S.A.

    Srikanth Vedamoorthy

  4. Lipomics and Lipid Signaling Laboratory, The Ohio State University, 611-A Davis Heart and Lung Research Institute, 473 West 12th Avenue, Columbus, OH, 43210, U.S.A.

    Narasimham Parinandi

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  1. Varadharaj Saradhadevi
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  2. Ramasamy Sakthivel
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  3. Srikanth Vedamoorthy
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  4. Ramasamy Selvam
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Correspondence to Narasimham Parinandi.

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Saradhadevi, V., Sakthivel, R., Vedamoorthy, S. et al. Alterations in band 3 protein and anion exchange in red blood cells of renal failure patients. Mol Cell Biochem 273, 11–24 (2005). https://doi.org/10.1007/s11010-005-5904-9

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