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Okadaic acid induces tyrosine phosphorylation of IκBα that mediated by PKR pathway in human osteoblastic MG63 cells

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Abstract

Treatment of human osteosarcoma cell line MG63 cells with okadaic acid stimulated phosphorylation of IκBα, as judged from the results of Western blot analysis and a λ protein phosphatase dephosphorylation assay. The stimulated phosphorylation of IκBα was both time- and dose-dependent. The phosphorylation sites of IκBα were taken to be tyrosine residues because the anti-phospho-tyrosine antibody bound to the samples immunoprecipitated with the anti-IκBα antibody. In the cells treated with 100 nM okadaic acid consequential translocation of NF-κB p65 from the cytosol to the nucleus occurred. Double-stranded RNA-dependent protein kinase (PKR) is a player in the cellular antiviral response and is involved in transcriptional stimulation through activation of NF-κB. We investigated the functional relationship between PKR and IκBα phosphorylation by constructing MG63 PKR K/R cells that produced a catalytically inactive mutant PKR. NF-κB p65 was detected in the nucleus of these cells, even in the unstimulated cells. Although IκBα was degraded phosphorylation of eIF-2α, a substrate of PKR, did not occur in the mutant cells treated with okadaic acid. Our results suggest that okadaic acid-induced tyrosine phosphorylation of IκBα was mediated by PKR kinase activity, thus indicating the involvement of this kinase in the control mechanism governing the activation of NF-κB. (Mol Cell Biochem 276: 211–217, 2005)

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Anatomy, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15, Kuramoto, Tokushima, 770-8504, Japan

    Hiroyuki Morimoto & Seiichiro Kitamura

  2. Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan

    Akiko Ozaki, Hirohiko Okamura, Kaya Yoshida & Tatsuji Haneji

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  1. Hiroyuki Morimoto
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  2. Akiko Ozaki
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  3. Hirohiko Okamura
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  6. Tatsuji Haneji
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Correspondence to Hiroyuki Morimoto.

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Morimoto, H., Ozaki, A., Okamura, H. et al. Okadaic acid induces tyrosine phosphorylation of IκBα that mediated by PKR pathway in human osteoblastic MG63 cells. Mol Cell Biochem 276, 211–217 (2005). https://doi.org/10.1007/s11010-005-4440-y

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  • DOI: https://doi.org/10.1007/s11010-005-4440-y

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