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Hybrid liposomes of DPPC/cholesterol/octyl-β-D-glucopyranoside with/without ibuprofen: thermal and morphological study

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Abstract

Investigation of new materials for biomedical applications has represented a relevant subject in the latest decade, enhancing versatile properties of lipids. It has been documented that the capabilities of lipid-based systems improve when they combine with polymers, proteins, and sugars. In this field, understanding the driving forces behind such hybrid systems is mandatory for biomedical applications. From this perspective, it is crucial to investigate the biophysical properties of this kind of material. Here, we investigate the biophysical properties of hybrid membranes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), cholesterol, and octyl-β-D-glucopyranoside (OGP). Lipid/sugar materials could have potential properties to use as nanovesicles for drug delivery. We encapsulate ibuprofen in lipid/sugar vesicles and evaluate their thermodynamics, hydrodynamics, and morphological properties by differential scanning calorimetry, dynamic light scattering, and scanning electron microscopy. We found that OGP combined with cholesterol modifies thermodynamic parameters of membranes such as phase transition temperature, enthalpy change, and cooperativity. Lipid vesicles containing OGP at 6.0 mM loaded with ibuprofen demonstrated good stability after 3 months of storage. Furthermore, electronic microscopy revealed the presence of well-defined liposomes. We conclude that cholesterol and OGP can act synergistically in polar–nonpolar spaces of the DPPC bilayer, where the hydrophobic nature of ibuprofen leads to incorporation into this hybrid core, which implies changes in the fluidity and compactness of the membrane occurring at temperatures of biological relevance. This investigation provides crucial knowledge regarding the biophysical properties of thermo-responsive biohybrid vesicles potentially to use in nanomedicine, which could be of practical reference for designing innovative drug delivery systems.

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Abbreviations

LUV:

Large unilamellar vesicles

T m :

Phase transition temperature

C p :

Specific heat capacity at constant pressure

∆H m :

Molar enthalpy change

ΔT 1/2 :

The width of the transition at half peak height

DPPC:

1,2-Dipalmitoyl-sn-glycero-3-phosphocholine

OGP:

Octyl-β-D-glucopyranoside

Ibu:

Ibuprofen

Chol:

Cholesterol

DSC:

Differential scanning calorimetry

DLS:

Dynamic light scattering

SEM:

Scanning electron microscopy

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Acknowledgements

RP-I thanks DGAPA-UNAM for a postdoctoral scholarship and acknowledges CONAHCYT—México for supporting in part the writing of this work via a postdoctoral fellowship, project number 3969865. This work was supported by FQ-UNAM (PAIP 5000-9020). We thank Rafael Ivan Puente Lee from USAII-UNAM for his competent assistance in SEM and Dr. Ismael Bustos Jaimes for the use of his Zetasizer equipment.

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  1. Centro de Investigación en Química Aplicada (CIQA), Enrique Reyna, 140, 25294, Saltillo, Coahuila, Mexico

    R. Pérez-Isidoro

  2. Laboratorio de Bio-Fisicoquímica, Departamento de Fisicoquímica, Facultad de Química, Universidad Nacional Autónoma de México, 04510, México City, Mexico

    A. Jessica Díaz-Salazar, S. Pérez-Casas & R. Pérez-Isidoro

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RP-I, SP-C, and AJD-S helped in conceptualization; RP-I and AJD-S helped in methodology and data analysis; RP-I and AJD-S helped in original draft preparation; RP-I, SP-C, and AJD-S contributed to review and editing; and RP-I, SP-C, and AJD-S worked in resources. All authors have read and agreed to the published version of the manuscript.

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Díaz-Salazar, A.J., Pérez-Casas, S. & Pérez-Isidoro, R. Hybrid liposomes of DPPC/cholesterol/octyl-β-D-glucopyranoside with/without ibuprofen: thermal and morphological study. J Therm Anal Calorim 148, 13983–13994 (2023). https://doi.org/10.1007/s10973-023-12704-1

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