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Radiosynthesis of 99mTc-nitrofurantoin a novel radiotracer for in vivo imaging of Escherichia coli infection

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Abstract

Nitrofurantoin (NFN) radiolabeling with technetium-99m (99mTc) was investigated using different concentration of the NFN, sodium pertechnetate (Na99mTcO4), reducing agent (SnCl2) at different pH ranges (5.1–6.00). The suitability of the 99mTc-NFN was evaluated in terms of the radiochemical purity (RCP) yield, in vitro stability in saline, serum, in vitro binding with E. coli, biodistribution in E. coli infected model rat (ERT), and scintigraphic accuracy in E. coli infected model rabbit (EBT). The superlative radiochemical succumb at 2.5 mg NFN, 125 μL of SnCl2 (1 μg/μL in 0.01 N HCl), 2.5 mCi of Na99mTcO4, at pH 5.2 at 30, 60, 90, and 120 min after reconstitution was 64.50 ± 0.11, 97.50 ± 0.16, 94.25 ± 0.10, 92.15 ± 0.14 and 90.75 ± 0.0.13%. The complex was found stable in saline and serum for 90% up to 120 min and showed 50–65% in vitro binding with E. coli. The absorption of the 99mTc-NFN, primarily at E. Coli infected (ECT) muscle of ERT was lower but after 60 min it went up to 7.25 ± 0.17%. The absorption in the blood, liver, spleen, stomach, intestines, inflamed muscle (N.T1) and normal muscle (N.T2) went down while in the kidney and urine it went up with time. The ratio of the ECT/N.T1 was 7:1 and N.T2/N.T1 was 2:1. The Whole Body Static (WBS) imaging of the ERB confirmed the suitability of the 99mTc-NFN as radiotracer. The superlative radiochemical succumb, significant in vitro stability in saline and serum, in vitro binding with E. coli, ideal biodistribution and scintigraphic accuracy confirmed the viability of the 99mTc-NFN as radiotracer for infection.

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Authors and Affiliations

  1. Nuclear Medicine Research Laboratory (NMRL), University of Peshawar, Peshawar, NWFP, Pakistan

    Syed Qaiser Shah

  2. Nuclear Medicine, Oncology and Radiotherapy Institute (NORI), Islamabad, Pakistan

    Aakif Ullah Khan

  3. Phytopharmaceutical & Neutraceuticals Research Laboratory (PNRL), University of Peshawar, Peshawar, NWFP, Pakistan

    Muhammad Rafiullah Khan

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  1. Syed Qaiser Shah
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  2. Aakif Ullah Khan
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  3. Muhammad Rafiullah Khan
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Correspondence to Syed Qaiser Shah.

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Shah, S.Q., Khan, A.U. & Khan, M.R. Radiosynthesis of 99mTc-nitrofurantoin a novel radiotracer for in vivo imaging of Escherichia coli infection. J Radioanal Nucl Chem 287, 417–422 (2011). https://doi.org/10.1007/s10967-010-0697-z

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  • DOI: https://doi.org/10.1007/s10967-010-0697-z

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