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Tx1, from Phoneutria nigriventer spidervenom, interacts with dihydropyridine sensitive-calcium channels in GH3 cells

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Summary

The aim of this work was to use the binding assay of tritiated-dihydropyridine and radioiodinated Tx1, isolated from the Phoneutria nigriventer venom, in order to show the presence of Cav1 calcium channels on pituitary tumour cell (GH3). We showed that GH3 cells have specific sites for 125I-Tx1, which are sensitive to nifedipine (~20%). Reverse competition assay with 3H-PN200-110 (40% inhibition) and electrophysiological data (50% inhibition) suggest that Cav1 calcium channels are target sites for this toxin. To summarize, Tx1 binds to specific sites on GH3 cells and this interaction results in Cav1 calcium channel blockade. 3H-PN200-110 and 125I-Tx1 binding assays proved to be useful tools to show the presence of calcium channels on GH3 cells.

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Authors and Affiliations

  1. Lab Radiobiologia, TR4, CDTN/CNEN, Cx Postal 941, 30123-970 Belo Horizonte, Brazil

    R. Gouv?a dos Santos

  2. Lab Radiobiologia, TR4, CDTN/CNEN, Cx Postal 941, 30123-970 Belo Horizonte, Brazil

    M. A. Soares

  3. Lab. Membranas Excitáveis, Depto Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, Brazil

    J. S. Cruz

  4. Lab. Membranas Excitáveis, Depto Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, Brazil

    R. Mafra

  5. Lab. Venenos e Toxinas Animais, Depto Bioquímica e Imunologia, ICB, UFMG, Belo Horizonte, Brazil

    R. Lomeo

  6. Centro de Pesquisa Prof. Carlos Ribeiro Diniz, Fundaç?o Ezequiel Dias, Belo Horizonte, Brazil

    M. N. Cordeiro

  7. Núcleo de Biomoléculas, ICB, UFMG, Belo Horizonte, Brazil

    A. M. Pimenta

  8. Lab. Venenos e Toxinas Animais, Depto Bioquímica e Imunologia, ICB, UFMG; Núcleo de Biomoléculas, ICB, UFMG, Belo Horizonte, Brazil

    M. E. De Lima

Authors
  1. R. Gouv?a dos Santos
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  2. M. A. Soares
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  3. J. S. Cruz
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  4. R. Mafra
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  5. R. Lomeo
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  6. M. N. Cordeiro
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  7. A. M. Pimenta
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  8. M. E. De Lima
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Gouv?a dos Santos, R., Soares, M., Cruz, J. et al. Tx1, from Phoneutria nigriventer spidervenom, interacts with dihydropyridine sensitive-calcium channels in GH3 cells. J Radioanal Nucl Chem 269, 585–589 (2006). https://doi.org/10.1007/s10967-006-0269-4

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  • DOI: https://doi.org/10.1007/s10967-006-0269-4

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