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Amino-functionalized mesoporous silica nanoparticles: adsorption and protection for pcDNA3.1(+)-PKB-HA

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Abstract

This work evaluated the adsorption and protection capacities of amino-functionalized mesoporous silica nanoparticles (Am-MSNs) for the recombinant DNA (rDNA), pcDNA3.1(+)-PKB-HA. The in vitro biocompatibility of Am-MSNs was also tested on HeLa and A549 cells, respectively. The results reveal that the MSNs modified with 3-aminopropyltriethoxysilane exhibit good adsorption capacity for pcDNA3.1(+)-PKB-HA at physiological conditions and the adsorption amount is higher than that of other silica nanoparticles. Moreover, the material can also protect pcDNA3.1(+)-PKB-HA from enzymatic degradation completely and presents high in vitro biocompatibility. This demonstrates that Am-MSNs may be potential nonviral vector candidates for pcDNA3.1(+)-PKB-HA.

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Acknowledgments

We acknowledge the financial support of the National Nature Science Foundation of China (50573030 and 21171065). We are grateful to Prof. Wei Shi for offering HeLa and A549 cells and pcDNA3.1(+)-PKB-HA. We also thank Prof. Zuo-ming Zhang for rDNA concentration measurement and Prof. Feng-xia Dong for zeta-potential measurement.

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Authors and Affiliations

  1. Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun, 130012, People’s Republic of China

    Hong Yang, Li Wang, Shubo Jing, Hao Huang, Jianing Xu & Ping Zhang

  2. Environmental and Bioengineering Technology College, Jilin Institute of Chemical Technology, Jilin , 132022, People’s Republic of China

    Kun Zheng

  3. Jilin Gongmao School, 37 Nonglin Street, Jilin, 132011, People’s Republic of China

    Hong Yang

Authors
  1. Kun Zheng
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  2. Hong Yang
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  3. Li Wang
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  4. Shubo Jing
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  5. Hao Huang
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  6. Jianing Xu
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  7. Ping Zhang
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Corresponding authors

Correspondence to Jianing Xu or Ping Zhang.

Additional information

Kun Zheng and Hong Yang contributed equally to this work.

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Zheng, K., Yang, H., Wang, L. et al. Amino-functionalized mesoporous silica nanoparticles: adsorption and protection for pcDNA3.1(+)-PKB-HA. J Porous Mater 20, 1003–1008 (2013). https://doi.org/10.1007/s10934-013-9679-1

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  • DOI: https://doi.org/10.1007/s10934-013-9679-1

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