Abstract
Garlic (Allium sativum) lectins are promising candidate molecules for the protection against chewing (lepidopteran) as well as sap sucking (homopteran) insect pests. Molecular mechanism of toxicity and interaction of lectins with midgut receptor proteins has been described in many reports. Lectins show its effect right from sensory receptors of mouth parts by disrupting the membrane integrity and food detection ability. Subsequently, enter into the gut lumen and interact with midgut glycosylated proteins like alkaline phosphatase (ALP), aminopeptidase-N (APN), cadherin-like proteins, polycalins, sucrase, symbionin and others. These proteins play critical role in life cycle of insect directly or indirectly. Lectins interfere with the activity of these proteins and causes physiological disorders leading to the death of insects. Lectins further transported across the insect gut, accumulated in various body parts (like haemolymph and ovary) and interact with intracellular proteins like symbionin and cytochrome p450. Binding with cytochrome p450 (which involve in ecdysone synthesis) might interfere in the development of insects, which results in growth retardation and pre-mature death.
Similar content being viewed by others
Abbreviations
- ALP:
-
Alkaline Phosphatase
- APN:
-
Aminopeptidase-N
- ASA:
-
Allium sativum agglutinin
- ASAL:
-
Allium sativum leaf agglutinin
- GNA:
-
Galanthus nivalis agglutinin
- BBMV:
-
Brush border membrane vesicles
References
Andersson JO (2000) Curr Biol 10:R866–R868
Angelucci C, Barrett-Wilt GA, Hunt DF, Akhurst RJ, East PD, Gordon KH, Campbell PM (2008) Insect Biochem Mol Biol 38:685–696
Angst BD, Marcozzi C, Magee AI (2001) J Cell Sci 114:629–641
Arenas I, Bravo A, Soberon M, Gomez I (2010) J Biol Chem 285:12497–12503
Arora A, Sharma HC, Dreissche EV, Sharma KK (2005) SAT eJournal/ejournal.icrisat.org. 1:1–3
Ashford DA, Smith WA, Douglas AE (2000) J Insect Physiol 46:335–341
Bachhawat K, Thomas CJ, Amutha B, Krishnasastryi MV, Khan MI, Surolia A (2001) J Biol Chem 276:5541–5546
Bandyopadhyay S, Roy A, Das S (2001) Plant Sci 161:1025–1033
Banerjee S, Hess D, Majumder P, Roy D, Das S (2004) J Biol Chem 279:23782–23789
Bharathi Y, VijayaKumar S, Pasalu IC, Balachandran SM, Reddy VD, Rao KV (2011) J Biotechnol 152:63–71
Blixt O, Head S, Mondala T, Scanlan C, Huflejt ME, Alvarez R, Bryan MC, Fazio F, Calarese D, Stevens J, Razi N, Stevens DJ, Skehel JJ, Van Die I, Burton DR, Wilson IA, Cummings R, Bovin N, Wong CH, Paulson JC (2004) Proc Natl Acad Sci USA 101:17033–17038
Burton SL, Ellar DJ, Li J, Derbyshire DJ (1999) J Mol Biol 287:1011–1022
Carlini CR, Grossi-de-Sab (2002) Toxicon 40:1515–1539
Chakraborti D, Sarkar A, Mondal HA, Das S (2009) Transgenic Res 18:529–544
Chandra NR, Ramachandraiah G, Bachhawat K, Dam TK, Surolia A, Vijayan M (1999) J Mol Biol 285:1157–1168
Chen SJ, Chen NT, Wang SH, Hsu JC, Ding WH, Kuo-Huang LL, Huang RN (2009) Pest Manag Sci 65:923–930
Cristofoletti PT, Ribeiro AF, Deraison C, Rahbe′ Y, Terra WR (2003) J Insect Physiol 49:11–24
Dam TK, Bachhawat K, Rani PG, Surolia A (1998) J Biol Chem 273:5528–5535
Denolf P, Hendrickx K, Van Damme J, Jansens S, Peferoen M, Degheele D, Van Rie J (1997) Eur J Biochem 248:748–761
Downing N (1978) J Exp Biol 77:247–250
Dunne J, Hanby AM, Poulsom R, Jones TA, Sheer D, Chin WG, Da SM, Zhao Q, Beverley PC, Owen MJ (1995) Genomics 30:207–223
Dutt U (2007) Environment News Service, 21 August, (countercurrents.org)
Dutta IP, Majumdar P, Saha P, Ray K, Das S (2005) Plant Sci 169:996–1007
Dutta I, Saha P, Majumder P, Sarkar A, Chakraborti D, Banerjee S, Das S (2005) Plant Biotech J 3:601–611
Filichkin SA, Brumfield S, Filichkin TP, Young MJ (1997) J Virol 71:569–577
Fisher DB (2000) In: Gruissem W, Jones RL, Buchanan BB (eds) Long distance transport. American Society of Plant Physiologists, Rockville, pp 730–784
Fitches E, Gatehouse AMR, Gatehouse JA (1997) J Insect Physiol 43:727–739
Fitches E, Ilett C, Gatehouse AMR, Gatehouse LN, Greene R, Edwards JP, Gatehouse JA (2001) J Insect Physiol 47:1389–1398
Fitches E, Philip J, Hinchliffe G, Vercruysse L, Chougule N, Gatehouse JA (2008) Insect Sci 15:483–495
Fitches E, Wiles D, Douglas AE, Hinchliffe G, Audsley N, Gatehouse JA (2008) Insect Biochem Mol Biol 38:905–915
Gatehouse AMR, Dewey FM, Dove J, Fenton KA, Pusztai A (1984) J Sci Food Agric 35:373–380
Gatehouse AMR, Powell KS, Van Damme EJM, Peumans W, Gatehouse JA (1995) In: Pusztai AJ, Bardocz S (eds) Lectins: biomedical perspectives. Taylor & Francis, London, pp 35–57
Gilbert LI, Warren JT (2005) Vitam Horm 73:31–57
Goldberg M, Peshkovsky C, Shifteh A, Al-Awqati Q (2000) J Biol Chem 275:24622–24629
Gómez I, Sánchez J, Miranda R, Bravo A, Sánchez M (2002) FEBS Lett 513:242–246
Gumbiner BM (1996) Cell 84:345–357
Habibi J, Backus EA, Czapla TC (1998) Cell Tissue Res 294:561–571
Habibi J, Backus EA, Huesing JE (2000) J Insect Physiol 46:611–619
Harper MS, Hopkins TL, Czapla TH (1998) Tissue Cell 30:166–176
Hilder VA, Powell KS, Gatehouse AMR, Gatehouse JA, Gatehouse LN, Shi Y, Hamilton WDO, Merryweather A, Newell CA, Timans JC (1995) Transgenic Res 4:18–25
Hooper NM (1994) FEBS Lett 354:1–6
Hossain DM, Shitomi Y, Moriyama K, Higuchi M, Hayakawa T, Mitsui T, Sato R, Hori H (2004) Appl Environ Microbiol 70:4604–4612
Hua G, Zhang R, Abdullah MA, Adang MJ (2008) Biochemistry 47:5101–5110
Ingle SS, Trivedi N, Prasad R, Kuruvilla J, Rao KK, Chhatpar HS (2001) Curr Microbiol 43:255–259
Jurat-Fuentes JL, Adang MJ (2004) Euro J Biochem 271:3127–3135
Jurat-Fuentes JL, Adang MJ (2007) J Invert Pathol 95:187–191
Karley AJ, Ashford DA, Minto LB, Pritchard J, Douglas AE (2005) J Insect Physiol 51:1313–1319
Kaur M, Singh K, Rup PJ, Kamboj SS, Saxena AK, Sharma M, Bhagat M, Sood SK, Singh J (2006) J Biochem Mol Biol 39:432–440
Knight PJ, Crickmore N, Ellar DJ (1994) Mol Microbiol 11:429–436
Knight PJ, Knowles BH, Ellar DJ (1995) J Biol Chem 270:17765–17770
Krishnamoorthy M, Jurat-Fuentes JL, McNall RJ, Andacht T, Adang MJ (2007) Insect Biochem Mol Biol 37:189–201
Laustsen PG, Vang S, Kristensen T (2001) Eur J Biochem 268:98–104
Lu YJ, Adang MJ (1996) Insect Biochem Mol Biol 26:33–40
Majumder P, Banerjee S, Das S (2004) Glycoconjugate J 20:525–530
Majumder P, Mondal HA, Das S (2005) J Agric Food Chem 53:6725–6729
Malik K, Mahmood T, Riazuddin S (2001) J Biol Sci 1:782–784
Mauchamp B, Royer C, Garel A, Jalabert A, Rocha MD, Grenier AM, Labas V, Vinh J, Mita K, Kadono K, Chavancy G (2006) Insect Biochem Mol Biol 36:623–633
McNall RJ, Adang MJ (2003) Insect Biochem Mol Biol 33:999–1010
Mondal HA, Chakraborti D, Majumder P, Roy P, Roy A, Bhattacharya SG, Das S (2011) PLoS ONE 6:e27716
Nakayama M, Nakajima D, Nagase T, Nomura N, Seki N, Ohara O (1998) Genomics 51:27–34
Nollet F, Kools P, Roy VF (2000) J Mol Biol 299:551–572
Ohizumi Y, Gaidamashvili M, Ohwada S, Matsuda K, Kominami J, Nakamura-Tsuruta S, Hirabayashi J, Naganuma T, Ogawa T, Muramoto K (2009) J Agric Food Chem 57:2896–2902
Peumans WJ, Van Damme EJM (1995) Plant Physiol 109:347–352
Pigott CR, Ellar DJ (2007) Microbiol Mol Biol Rev 71:255–281
Powell KS (2001) Entomol Exp Appl 99:71–78
Powell KS, Gatehouse AMR, Hilder VA, van Damme EJM, Peumans WJ, Boonjawat J, Horsham K, Gatehouse JA (1995) Entomol Exp Appl 75:61–65
Powell KS, Spence J, Bharathi M, Gatehouse JA, Gatehouse AMR (1998) J Insect Physiol 44:529–539
Ramachandraiah G, Chandra NR (2000) Proteins Struc Func Genet 39:358–364
Ramachandraiah G, Chandra NR, Surolia A, Vijayan M (2002) Acta Cryst D58:414–420
Ramachandraiah G, Chandra NR, Surolia A, Vijayan M (2003) Glycobiology 13:765–775
Rao KV, Rathore KS, Hodges TK, Fu X, Stoger E, Sudhakar D, Williams S, Christou P, Bharathi M, Bown DP, Powell KS, Spence J, Gatehouse AM, Gatehouse JA (1998) Plant J 15:469–477
Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Insect Biochem Mol Biol 36:188–199
Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Mol Cell Endocrinol 247:166–174
Rewitz KF, Rybczynski R, Warren JT, Gilbert LI (2006) Biochem Soc Trans 34:1256–1260
Rewitz KF, Connor MB, Gilbert LI (2007) Insect Biochem Mol Biol 37:741–753
Rhodes JD, Croghan PC, Dixon AFG (1997) Physiol Entomol 22:373–379
Sadeghi A, Smagghe G, Broeders S, Hernalsteens JP, DeGreve H, Peumans WJ, VanDamme EJ (2008) Transgenic Res 17:9–18
Saha P, Dasgupta I, Das S (2006) Plant Mol Biol 62:735–752
Sarkar A, Hess D, Mondal HA, Banerjee S, Sharma HC, Das S (2009) J Prot Res 8:1838–1848
Sauvion N, Charles H, Febvay G, Rahbe Y (2004) Entomol Exp Appl 110:31–44
Smeets K, Van Damme EJM, Verhaert P, Barre A, Rouge P, Van Leuven F, Peumans WJ (1997) Plant Mol Biol 33:223–234
Takesue S, Yokota K, Miyajima S, Taguchi R, Ikezawa H, Takesue Y (1992) Comp Biochem Physiol B 102:7–11
Upadhyay SK, Chandrashekar K, Thakur N, Verma PC, Borgio JF, Singh PK, Tuli R (2011) J Biosci 36:153–161
Upadhyay SK, Mishra M, Singh H, Ranjan A, Chandrashekar K, Verma PC, Singh PK, Tuli R (2010) Proteomics 10:4431–4440
Upadhyay SK, Saurabh S, Rai P, Singh R, Chandrashekar K, Verma PC, Singh PK, Tuli R (2010) J Biotechnol 146:1–8
Upadhyay SK, Saurabh S, Singh R, Rai P, Dubey NK, Chandrashekar K, Negi KS, Singh PK, Tuli R (2011c) Protein J. doi:10.1007/s10930-011-9342-0
Upadhyay SK, Singh P K (2011b) Biotechnol Lett. doi: 10.1007/s10529-011-0665-x
Upadhyay SK, Singh S, Chandrashekar K, Tuli R, Singh P K (2012) Appl Microbiol Biotechnol. doi: 10.1007/s00253-011-3547-1
Usui T, Shima Y, Shimada Y, Hirano S, Burgess RW, Schwarz TL, Takeichi M, Uemura T (1999) Cell 98:585–595
Vadlamudi RK, Ji TH, Bulla LA Jr (1993) J Biol Chem 268:12334–12340
Vadlamudi RK, Weber E, Ji I, Ji TH, Bulla LA Jr (1995) J Biol Chem 270:5490–5494
Van Damme EJM, Barre A, Rougé P, Peumans WJ (2004) Trends Plant Sci 9:484–489
Van Damme EJM, Lannoo N, Peumans WJ (2008) Adv Bot Res 48:107–209
Van Damme EJM, Peumans WJ, Barre A, Rougé P (1998) CRC Crit Rev Plant Sci 17:575–692
Van Damme EJM, Smeets K, Torrekens S, Van Leuven F, Goldstein IJ, Peumans WJ (1992) Eur J Biochem 206:413–420
Van Driessche E, Fischer J, Beeckmans S, Bog-Hanse TC (1996) Lectins, biology, biochemistry, clinical biochemistry, Textop, Denmark, vol 11
Vasconcelos IM, Oliveira JTA (2004) Toxicon 44:385–403
Vestal DJ, Ranscht B (1992) J Cell Biol 119:451–461
Virla EG, Casuso M, Frias EA (2010) Crop protection 29:635–638
Walters FS, Mullin CA (1988) Arch Insect Biochem Physiol 9:35–46
Wang P, Zhang X, Zhang J (2005) Insect Biochem Mol Biol 35:611–620
Warren JT, Petryk A, Marques G, Parvy JP, Shinoda T, Itoyama K, Kobayashi J, Jarcho M, Li Y, O’Connor MB, Dauphin-Villemant C, Gilbert LI (2004) Insect Biochem Mol Biol 34:991–1010
Wilkinson TL, Ashford DA, Pritchard J, Douglas AE (1997) J Exp Biol 200:2137–2143
Wolfersberger MG (1984) Am Zool 24:187–197
Yarasi B, Sadumpati V, Immanni CP, Vudem DR, Khareedu VR (2008) BMC Plant Biol 8:102
Young MJ, Filichkin SA (1999) Trends Microbiol 7:346–347
Acknowledgment
Authors are grateful to the Council of Scientific and Industrial Research, Government of India for funding the research. SKU is thankful to CSIR for senior research fellowship and G B Technical University, Lucknow, UP, India for Ph.D. registration.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Upadhyay, S.K., Singh, P.K. Receptors of Garlic (Allium sativum) Lectins and Their Role in Insecticidal Action. Protein J 31, 439–446 (2012). https://doi.org/10.1007/s10930-012-9423-8
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10930-012-9423-8