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A Comparative Study with Colchicine on Glutathione Reductase

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Abstract

Colchicine is a drug used for the treatment of FMF, primary biliary cirrhosis, psoriasis, Behçet’s disease, aphthous stomatitis. Glutathione reductase (GR; E.C 1.6.4.2) is a crucial enzyme which reduces glutathione disulphide to the sulfhydryl form GSH by the NADPH-dependent reduction, which is an important cellular antioxidant system. The purpose of the present work is to evaluate the in vitro effects of colchicine on GR from various sources. The component of glutathione redox cycle, GR, plays important role in the protection of the cell from the toxic effects of reactive oxygen species. Due to its significance the enzyme has been purified from a number of animals, plants and microbial sources and studied the in vitro effects of many chemical compounds or drugs on enzyme activity. We have established that colchicine inhibits GR in a concentration dependent manner. We have investigated the kinetic characterization, inhibition types and constants (Ki).

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Abbreviations

NADPH:

Nicotinamide adenine dinucleotide phosphate reduced form

GSSG:

Oxidized glutathione

GR:

Glutathione reductase

Ki:

Inhibition constant

GSH:

Reduced glutathione

References

  1. Acan NL, Tezcan EF (1989) FEBS Lett 250:72–74

    Article  CAS  Google Scholar 

  2. Andrews P (1965) Biochem J 98:595

    Google Scholar 

  3. Arscott LD, Veine DM, Williams CH Jr (2000) Biochemistry 39(16):4711–4721

    Article  CAS  Google Scholar 

  4. Ben-Chetrit E, Levy M (1998) Semin Arthritis Rheum 28:48–59

    Article  CAS  Google Scholar 

  5. Bhat A, Naguwa SM, Cheema GS, Gershwin ME (2009) Ann N Y Acad Sci 1173:766–773

    Article  CAS  Google Scholar 

  6. Bradford MM (1976) Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  7. Cerquaglia C, Diaco M, Nucera G, La Regina M, Montalto M, Manna R (2005) Curr Drug Targets Inflamm Allergy 4:117–124

    Article  CAS  Google Scholar 

  8. Laemli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  Google Scholar 

  9. Molad Y (2002) Curr Rheumatol Rep 4:252–256

    Article  Google Scholar 

  10. Picaud T, Desbois A (2006) Biochemistry 45(51):15829–15837

    Google Scholar 

  11. Pope RM, Tschopp J (2007) Arthritis Rheum 56:3183–3188

    Article  CAS  Google Scholar 

  12. Rahman Q, Abidi P, Afaq F, Schiffmann D, Mossman BT, Kamp DW, Athar M (1999) Crit Rev Toxicol 29(6):543–568

    Article  CAS  Google Scholar 

  13. Rigante D, La Torraca I, Avallone L, Pugliese AL, Gaspari S, Stabile A (2006) Eur Rev Med Pharmacol Sci 10(4):173–178

    CAS  Google Scholar 

  14. Ruiz-Gómez MJ, Souviron A, Martínez-Morillo M, Gil L (2000) J Physiol Biochem 56(4):307–312

    Article  Google Scholar 

  15. Segel IH (1975) Enzyme kinetics, 3rd edn. Chapter 9 & 11, Wiley, Toronto, pp 273

  16. Stapczynski S, Rothstein RJ, Gaye WA, Niemann JT (1981) Ann Emerg Med 10:364–369

    Article  CAS  Google Scholar 

  17. Tandogan B, Ulusu NN (2006) FABAD J Pharm Sci 31:230–237

    Google Scholar 

  18. Tandogan B, Ulusu NN (2010) Protein Pept Lett. Jan 10 (Epub ahead of print)

  19. Ulusu NN, Tandoğan B (2007) Mol Cell Biochem 303(1–2):45–51

    Article  CAS  Google Scholar 

  20. Wiesenfeld PL, Garthoff LH, Sobotka TJ, Suagee JK, Barton CN (2007) J Appl Toxicol 27(5):421–433

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work is a part of a project and (0701101011) supported by Hacettepe University Scientific Research Unit.

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Authors and Affiliations

  1. Faculty of Medicine, Department of Biochemistry, Hacettepe University, 06100, Ankara, Turkey

    Berivan Tandogan & N. Nuray Ulusu

Authors
  1. Berivan Tandogan
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  2. N. Nuray Ulusu
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Correspondence to N. Nuray Ulusu.

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Tandogan, B., Ulusu, N.N. A Comparative Study with Colchicine on Glutathione Reductase. Protein J 29, 380–385 (2010). https://doi.org/10.1007/s10930-010-9263-3

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