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Fabrication of a Novel Fibrous Mat Based on Gliadin/Ethylcellulose Incorporated with Triamcinolone for Treatment of Oral Ulcers

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Abstract

Eelectrospun fibrous mats for oral ulcer dressing should be able to attach oral mucosa in a high moisture environment and provide desirable therapeutic effects. Ethylcellulose and Gliadin are appropriate polymers in this regard. In the current study, first, Triamcinolone was integrated into Gliadin/Ethylcellulose fibrous mats (Triamcinolone/Gliadin/Ethylcellulose). Then, the chemical structure, thermal stability, mechanical properties, and morphology of Triamcinolone/Gliadin/Ethylcellulose fibrous mats were evaluated by Fourier Transform Infrared Spectroscopy, thermal gravimetric analysis (TGA), Tensile stress analysis, and scanning electron microscopy, respectively. Moreover, the biocompatibility, anti-inflammatory, and antioxidative effects of these fibrous mats were evaluated on human gingival fibroblasts. The results of SEM confirmed the uniform structure of the fabricated fibrous mats. The mechanical strength and the thermal stability of fibrous mats containing Triamcinolone were improved compared with Gliadin/Ethylcellulose fibrous mats. In vitro analysis showed the non-cytotoxicity of these fibrous mats on HGFs. Moreover, expression levels of TNF α and IL 6 in fabricated mats were 0.995 pg/mL and 0.44 pg/mL, which were significantly less than the control group. Also, the measurement of ROS activity showed significant decreases in stimulated HGFs. According to these results, it seems that the Triamcinolone/Gliadin/Ethylcellulose would be a practical mat for application in the treatment of oral inflammatory ulcers.

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Acknowledgements

The authors would like to thank the “Stem Cell Research Center, Tabriz University of Medical Sciences” and “Sara Research Laboratory” for their kindly cooperation.

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  1. Mahdieh Alipour and Marziyeh Aghazadeh have contributed equally.

Authors and Affiliations

  1. Dental and Periodontal Research Center, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahdieh Alipour

  2. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mahdieh Alipour, Marziyeh Aghazadeh & Zahra Aghazadeh

  3. Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Marjan Ghorbani

  4. Department of Oral Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

    Marziyeh Aghazadeh, Sana Taherzadeh & Zahra Aghazadeh

  5. Nanofiber Research Center, Asian Nanostructures Technology Co. (ANSTCO), Zanjan, Iran

    Soghra Ramezani

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  1. Mahdieh Alipour
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Correspondence to Marjan Ghorbani or Zahra Aghazadeh.

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The study procedure was approved by the Ethical Committee of Tabriz University of Medical Science (Approval ID: 66362).

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Alipour, M., Aghazadeh, M., Ramezani, S. et al. Fabrication of a Novel Fibrous Mat Based on Gliadin/Ethylcellulose Incorporated with Triamcinolone for Treatment of Oral Ulcers. J Polym Environ 30, 2579–2588 (2022). https://doi.org/10.1007/s10924-021-02365-2

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