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The Production of Polyhydroxybutyrate by Two-Step Fermentation and the Application of Polyhydroxybutyrate as a Novel Substrate for a Biolubricant

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Abstract

Two-step fermentation was utilized to increase PHB production. Cupriavidus necator was firstly cultivated in molasses medium for 144 h. Afterward, cells were recovered and extracted for PHB. The highest biomass (2.10 g/L) and PHB (62.38% of DCW) were obtained in the first reactor after 108 h of incubation. Filtrate after cell recovery were overflowed to the second reactor. Filtrate after cell recovery contained sugar and organic acids. Therefore, it was suitable to be re-utilized as substrate for PHB. In the second reactor, 10% of C. necator was inoculated and incubated for 120 h. The highest biomass (1.67 g/L) and PHB (67.66% of DCW) were achieved after 96 h of incubation. PHB was further utilized as substrate for biolubricant. The production of biolubricant involved a two-stage transesterification. The biolubricant from PHB passed the ISO VG-15 commercial standards for hydraulic systems and many other industrial applications.

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Acknowledgements

The authors would like to thank Thailand Research Fund (TRF) Grant (Project Number RTA6080010) and the Research and Development Institute at Thaksin University for financial support. Finally, we would like to thank Mr. Christopher Joseph Forti (English adviser and English proof-reader, Thaksin University) for useful comments and suggestions on the language and structure of our manuscript.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Thaksin University, Pa Phayom, Phatthalung, 93210, Thailand

    Piyatida Keunun, Thanaphorn Rakkarn, Tewan Yunu, Nisa Paichid & Kanokphorn Sangkharak

  2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla, 93110, Thailand

    Poonsuk Prasertsan

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  1. Piyatida Keunun
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  2. Thanaphorn Rakkarn
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  3. Tewan Yunu
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  4. Nisa Paichid
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  6. Kanokphorn Sangkharak
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Correspondence to Kanokphorn Sangkharak.

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Keunun, P., Rakkarn, T., Yunu, T. et al. The Production of Polyhydroxybutyrate by Two-Step Fermentation and the Application of Polyhydroxybutyrate as a Novel Substrate for a Biolubricant. J Polym Environ 26, 2459–2466 (2018). https://doi.org/10.1007/s10924-017-1140-0

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  • DOI: https://doi.org/10.1007/s10924-017-1140-0

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