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A Green Biosensing Matrix Based on Chitosan and Graphene nanohybrid for the Immobilization of Glucose Oxidase: Synthesis and Property evaluation

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Abstract

A promising green biosensing material, nano-cerium oxide (CeO2) decorated reduced graphene oxide (RGO) nanohybrid reinforced chitosan (CS) nanocomposite has been synthesized as a transducer phase for the effective immobilization of glucose oxidase (GOx) enzyme. CeO2 nanoparticles were grown on the RGO nanosheet by facile hydrothermal treatment. The effective growth of synthesized nano-CeO2 on the RGO was evident from X-ray diffraction, scanning electron microscopy and transmission electron microscopy and Raman analysis. Morphological studies confirmed the effective immobilization of GOx on the chitosan nanocomposites modified FTO electrode. The electrocatalytic response of the GOx/nano-CeO2–RGO/CS/FTO bioelectrode was investigated using electrochemical impedance and cyclic voltammetry studies. The obtained results indicate that as compared with bare CeO2 nanoparticles and RGO, the nano-CeO2/RGO nanohybrid shows significant electrochemical activity and provides an adequate microenvironment for effective enzyme immobilization due to the excellent synergism between the CeO2 nanoparticles and RGO nanosheet.

Graphical Abstract

Scheme 1: Schematic diagram of graphene nanohybrid reinforced Chitosan nanocomposite modified biosensing matrix.

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Acknowledgments

One of the author Miss Sriparna De thanks Mrs. Lakshmi Unnikrishnan and Mr. K. Prabakaran from LARPM, CIPET for the support of the electrochemical characterization. This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Central Institute of Plastics Engineering and Technology (CIPET), Chennai, India

    Sriparna De, Smita Mohanty & Sanjay Kumar Nayak

  2. Laboratory for Advanced Research in Polymeric Materials (LARPM), CIPET, Bhubaneswar, 751 024, Odisha, India

    Smita Mohanty & Sanjay Kumar Nayak

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  1. Sriparna De
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  2. Smita Mohanty
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  3. Sanjay Kumar Nayak
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Correspondence to Smita Mohanty.

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De, S., Mohanty, S. & Nayak, S.K. A Green Biosensing Matrix Based on Chitosan and Graphene nanohybrid for the Immobilization of Glucose Oxidase: Synthesis and Property evaluation. J Inorg Organomet Polym 25, 1332–1344 (2015). https://doi.org/10.1007/s10904-015-0245-y

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  • DOI: https://doi.org/10.1007/s10904-015-0245-y

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