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Rhodamine-based Fluorescent Probe With Quick Response and High Selectivity for Imaging Labile Ferrous Iron in Living Cells and Zebrafish

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Abstract

The transition between its various oxidation states of Iron plays a crucial part in various chemical transformation of cells. Misregulation of iron can give rise to the iron-catalyzed reactive oxygen species disorder which have been linked to a variety of diseases, so it is crucial to monitor the labile iron pool in vivo for clinical diagnosis. According to iron autoxidation and hydrogen abstraction reaction, we reported a novel “off-on” fluorescent probe to response to ferrous (Fe2+) both in solutions and biological systems. The probe responds to Fe2+ with good selectivity toward competing metal ions. What’s more, the probe presents significant fluorescent enhancement to Fe2+ in less than 1 min, making real-time sensing in biological system possible. The applications of the probe in bioimaging revealed the changes in labile iron pool by iron autoxidation or diverse stimuli.

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Data Availability

The datasets generated and analyzed during the current study are available in the supplementary material.

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Funding

The financial support provided by Jilin Provincial of Science Technology Department (20220203020SF), the National Natural Science Foundation of China (22106051).

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Authors and Affiliations

  1. Center of Characterization and Analysis, Jilin Institute of Chemical Technology, Jilin, People’s Republic of China

    Shanshan Wang, Zhigang Liu & Xiaodan Zeng

  2. Jinan Stomatol Hosp, Periodont & Oral Med Dept, Jinan, Shandong, People’s Republic of China

    Jing Fu

  3. School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin, People’s Republic of China

    Xin Chen & Shihua Yu

Authors
  1. Shanshan Wang
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Contributions

XDZ and JF participated in the study design, the results discussion and revised the manuscript. SSW and XC participated in the study design, the results discussion, manuscript preparation, and revision. SHY conducted the practical work, participated in the results discussion, preparation and writing of the manuscript first draft. ZGL participated in the study design, the results discussion, manuscript preparation, revision and submission.

Corresponding authors

Correspondence to Jing Fu or Xiaodan Zeng.

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Wang, S., Chen, X., Liu, Z. et al. Rhodamine-based Fluorescent Probe With Quick Response and High Selectivity for Imaging Labile Ferrous Iron in Living Cells and Zebrafish. J Fluoresc (2023). https://doi.org/10.1007/s10895-023-03551-2

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