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Smartphone-Aided Fluorescence Detection of Cardiac Biomarker Myoglobin by a Ratiometric Fluorescent AuNCs-QDs Nanohybrids Probe with High Sensitivity

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Abstract

Simple and sensitive detection of cardiac biomarkers is of great significance for early diagnosis and prevention of acute myocardial infarction (AMI). Here, a ratiometric fluorescent nanohybrids probe (AuNCs-QDs) was synthesized through the coupling of bovine serum albumin-functionalized gold nanoclusters (AuNCs) with CdSe/ZnS quantum dots (QDs) to realize simple and sensitive detection of cardiac biomarker myoglobin (Mb). The AuNCs-QDs probe shows pink fluorescence under UV light, with two emission peaks at 468 nm and 630 nm belonging to QDs and AuNCs, respectively. Importantly, the presence of Mb caused fluorescence quenching of the blue-emitting QDs, thereby inhibiting the fluorescence resonance energy transfer (FRET) process between QDs and AuNCs, and reducing the fluorescence intensity ratio (F468/F630) of AuNCs-QDs probe effectively. As the concentration of Mb increases, the ratiometric fluorescent probe also exhibits a visible fluorescence color change. The detection limit was as low as 4.99 μg/mL, and the response of the probe to Mb showed a good linear relationship up to 0.52 mg/mL. Moreover, the probe has excellent specificity for Mb. Besides, the AuNCs-QDs has been applied to detect Mb of urine samples. More importantly, we also developed an AuNCs-QDs probe modified smartphone-aided paper-based strip for on-site monitoring of Mb. As far as we know, this is the first report of a smartphone-aided paper-based strip for on-site quick monitoring of Mb, which provides a useful approach for AMI biomarker monitoring and may can be extended to other medical diagnostics.

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References

  1. Anderson JL, Morrow DA (2017) Acute myocardial infarction. N Engl J Med 376:2053–2064. https://doi.org/10.1056/NEJMra1606915

    Article  CAS  PubMed  Google Scholar 

  2. White HD, Thygesen K, Alpert JS (2007) Universal definition of myocardial infarction. J Am Coll Cardiol 50:2173–2195. https://doi.org/10.1093/eurheartj/ehn130

    Article  PubMed  Google Scholar 

  3. de Haan JJ, Smeets MB, Pasterkamp G, Arslan Danger F (2013) Signals in the initiation of the inflammatory response after myocardial infarction. Mediators Inflamm 2013:206039–206051. https://doi.org/10.1155/2013/206039

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Mythili S, Malathi N (2015) Diagnostic markers of acute myocardial infarction. Biomed Rep 3:743–748. https://doi.org/10.3892/br.2015.500

    Article  PubMed  PubMed Central  Google Scholar 

  5. Lim WY, Thevarajah TM, Goh BT, Khor SM (2019) Paper microfluidic device for early diagnosis and prognosis of acute myocardial infarction via quantitative multiplex cardiac biomarker detection – ScienceDirect. Biosens Bioelectron 128:176–185. https://doi.org/10.1016/j.bios.2018.12.049

    Article  CAS  PubMed  Google Scholar 

  6. Chan CPY, Wan TSM, Watkins KL, Pelsers MMAL, Van der Voort D, Tang FPW, Lam KHK, Mill J, Yuan Y, Lehmann M, Hempel A, Sanderson JE, Glatz JFC, Renneberg R (2015) Rapid analysis of fatty acid-binding proteins with immunosensors and immunotests for early monitoring of tissue injury. Biosens Bioelectron 20:2566–2580. https://doi.org/10.1016/j.bios.2004.09.028

    Article  CAS  Google Scholar 

  7. Suprun EV, Shilovskaya AL, Lisitsa AV, Bulko TV, Shumyantseva VV, Archakov AI (2011) Electrochemical immunosensor based on metal nanoparticles for cardiac myoglobin detection in human blood plasma. Electroanalysis 23:1051–1057. https://doi.org/10.1002/elan.201000668

    Article  CAS  Google Scholar 

  8. Moreira FTC, Sharma S, Dutra RAF, Noronha JPC, Cass AEG, Sales MGF (2015) Detection of cardiac biomarker proteins using a disposable based on a molecularly imprinted polymer grafted onto graphite. Microchim Acta 182:975–983. https://doi.org/10.1007/s00604-014-1409-0

    Article  CAS  Google Scholar 

  9. Osman B, Uzun L, Beşirli N, Denizli A (2013) Microcontact imprinted surface plasmon resonance sensor for myoglobin detection. Mater Sci Eng C 33:3609–3614. https://doi.org/10.1016/j.msec.2013.04.041

    Article  CAS  Google Scholar 

  10. Gnedenko OV, Mezentsev YV, Molnar AA, Lisitsa AV, Ivanov AS, Archakov AI (2013) Highly sensitive detection of human cardiac myoglobin using a reverse sandwich immunoassay with a gold nanoparticle-enhanced surface plasmon resonance biosensor. Anal Chim Acta 759:105–109. https://doi.org/10.1016/j.aca.2012.10.053

    Article  CAS  PubMed  Google Scholar 

  11. Liu T, Fu L, Yin C, Wu M, Chen L, Niu N (2022) Design of smartphone platform by ratiometric fluorescent for visual detection of silver ions. Microchem J 174:107016–107024. https://doi.org/10.1016/J.MICROC.2021.107016

    Article  CAS  Google Scholar 

  12. Zhang Y, Yang M, Shao Z, Xu H, Liao X (2020) A paper-based fluorescent test for determination and visualization of cysteine and glutathione by using gold-silver nanoclusters. Microchem J 158:105327–105334. https://doi.org/10.1016/j.microc.2020.105327

    Article  CAS  Google Scholar 

  13. Li H, Lin H, Lv W, Gai P, Li F (2020) Equipment-free and visual detection of multiple biomarkers via an aggregation induced emission luminogen-based paper biosensor. Biosens Bioelectron 165:112336–112343. https://doi.org/10.1016/j.bios.2020.112336

    Article  CAS  PubMed  Google Scholar 

  14. Wang J, Ren L, Wang X, Wang Q, Wan Z, Li L, Liu W, Wang X, Li M, Tong D, Liu A, Shang B (2009) Superparamagnetic microsphere-assisted fluoroimmunoassay for rapid assessment of acute myocardial infarction. Biosens Bioelectron 24:3097–3102. https://doi.org/10.1016/j.bios.2009.03.033

    Article  CAS  PubMed  Google Scholar 

  15. Shorie M, Kumar V, Sabherwal P, Ganguli A (2015) Carbon quantum dots-mediated direct fluorescence assay for the detection of cardiac marker myoglobin. Curr Sci 108:1595–1596. http://www.jstor.org/stable/24905522. Accessed 10 May 2023

  16. Liu D, Zeng Y, Zhou G, Lu X, Miao D, Yang Y, Zhai Y, Zhang J, Zhang Z, Wang H (2019) Fluorometric determination of cardiac myoglobin based on energy transfer from a pyrene-labeled aptamer to graphene oxide. Microchim Acta 186:1–6. https://doi.org/10.1007/s00604-019-3385-x

    Article  CAS  Google Scholar 

  17. Gui R, Jin H, Bu X, Fu Y, Wang Z, Liu Q (2019) Recent advances in dual-emission ratiometric fluorescence probes for chemo/biosensing and bioimaging of biomarkers. Coord Chem Rev 383:82–103. https://doi.org/10.1016/j.ccr.2019.01.004

    Article  CAS  Google Scholar 

  18. Zhang Y, Xu H, Chen Y, You X, Pu Y, Xu W, Liao X (2020) High-sensitivity detection of cysteine and glutathione using Au nanoclusters based on aggregation-induced emission. J Fluoresc 30:1491–1498. https://doi.org/10.1007/s10895-020-02618-8

    Article  CAS  PubMed  Google Scholar 

  19. Yang J, Huang Y, Cui H, Li L, Ding YP (2022) A FRET fluorescent sensor for ratiometric and visual detection of sulfide based on carbon dots and silver nanoclusters. J Fluoresc 32:1815–1823. https://doi.org/10.1007/s10895-022-02981-8

    Article  CAS  PubMed  Google Scholar 

  20. Fu Q, Zhou X, Wang M, Su X (2022) Nanozyme-based sensitive ratiometric fluorescence detection platform for glucose. Anal Chim Acta 1216:339993–340000. https://doi.org/10.1016/J.ACA.2022.339993

    Article  CAS  PubMed  Google Scholar 

  21. Yu X, Zhang C, Zhang L, Xue Y, Li H, Wu Y (2018) The Construction of a FRET Assembly by Using Gold Nanoclusters and Carbon Dots and their Application as a Ratiometric Probe for Cysteine Detection. Sens Actuators B Chem 263:327–335. https://doi.org/10.1016/j.snb.2018.02.072

    Article  CAS  Google Scholar 

  22. Shi G, Zhu A, Qi Y, Zhang M (2015) Terbium(III)/gold nanocluster conjugates: the development of a novel ratiometric fluorescent probe for mercury(II) and a paper-based visual sensor. Analyst 140:5656–5661. https://doi.org/10.1039/c5an00802f

    Article  PubMed  Google Scholar 

  23. Liu W, Wang X, Wang Y, Li J, Shen D, Kang Q, Chen L (2018) Ratiometric fluorescence sensor based on dithiothreitol modified carbon dots-gold nanoclusters for the sensitive detection of mercury ions in water samples. Sens Actuators B Chem 262:810–817. https://doi.org/10.1016/j.snb.2018.01.222

    Article  CAS  Google Scholar 

  24. Huang X, Jiang H, Li Y, Sang L, Zhou H, Shahzad SA, Ibupoto ZH, Yu C (2017) Synthesis of silica nanoparticles doped with [Ru(bpy)3]2+ and decorated with silver nanoclusters for the ratiometric photoluminescent determination and intracellular imaging of Cu(II) ions. Microchim Acta 184:2325–2331. https://doi.org/10.1007/s00604-017-2206-3

    Article  CAS  Google Scholar 

  25. Wang C, Lin H, Xu Z, Huang Y, Humphrey MG, Zhang C (2016) Tunable carbon-dot-based dual-emission fluorescent nanohybrids for ratiometric optical thermometry in living cells. ACS Appl Mater Inter 8:6621–6628. https://doi.org/10.1021/acsami.5b11317

    Article  CAS  Google Scholar 

  26. Niu W, Shan D, Zhu R, Deng S, Cosnier S, Zhang X (2016) Dumbbell-shaped carbon quantum dots/AuNCs nanohybrid as an efficient ratiometric fluorescent probe for sensing cadmium (II) ions and l -ascorbic acid. Carbon 96:1034–1042. https://doi.org/10.1016/j.carbon.2015.10.051

    Article  CAS  Google Scholar 

  27. Han B, Li Y, Hu X, Yan Q, Jiang J, Yu M, Peng T, He G (2018) Paper-based visual detection of silver ions and L-cysteine with a dual-emissive nanosystem of carbon quantum dots and gold nanoclusters. Anal Methods 10:3945–3950. https://doi.org/10.1039/C8AY01044G

    Article  CAS  Google Scholar 

  28. Wen Z, Song S, Hu T, Wang C, Qu F, Wang P, Yang M (2019) A dual emission nanocomposite prepared from copper nanoclusters and carbon dots as a ratiometric fluorescent probe for sulfide and gaseous H2S. Microchim Acta 186:1–8. https://doi.org/10.1007/s00604-019-3295-y

    Article  CAS  Google Scholar 

  29. Wang X, Duan Q, Zhang B, Cheng X, Wang S, Sang S (2021) Ratiometric fluorescence detection of Cd2+ based on N. S co-doped carbon quantum dots/Au nanoclusters. Microchem J 167:106269–106275. https://doi.org/10.1016/j.microc.2021.106269

    Article  CAS  Google Scholar 

  30. Ge H, Zhang K, Yu H, Yue J, Yu L, Chen X, Hou T, Alamry KA, Marwani HM, Wang S (2018) Sensitive and selective detection of antibiotic D-penicillamine based on a dual-mode probe of fluorescent carbon dots and gold nanoparticles. J Fluoresc 28:1405–1412. https://doi.org/10.1007/s10895-018-2307-3

    Article  CAS  PubMed  Google Scholar 

  31. Zhang Y, Xu H, Yang Y, Zhu F, Pu Y, You X, Liao X (2021) Efficient fluorescence resonance energy transfer-based ratiometric fluorescent probe for detection of dopamine using a dual-emission carbon dot-gold nanocluster nanohybrid. J Photochem Photobiol A 411:113195–113202. https://doi.org/10.1016/j.jphotochem.2021.113195

    Article  CAS  Google Scholar 

  32. Wang N, Ga L, Ai J (2018) Synthesis of novel fluorescent copper nanomaterials and their application in detection of iodide ions and catalysis. Anal Methods 11:44–48. https://doi.org/10.1039/C8AY01871E

    Article  Google Scholar 

  33. Mathew A, Pradeep T (2015) Noble metal clusters: applications in energy, environment, and biology. Part Part Syst Charact 31:1017–1053. https://doi.org/10.1002/ppsc.201400033

    Article  CAS  Google Scholar 

  34. Al-mashriqi HS, Cai M, Qi S, Zhai HL (2023) BSA capped gold nanoclusters modulated by copper ion for sensitive and selective detection of histidine in biological fluid. J Fluoresc 33:697–706. https://doi.org/10.1007/s10895-022-03112-z

    Article  CAS  PubMed  Google Scholar 

  35. Qiu Q, Gao R-R, Xie A, Jiao Y, Dong W (2020) A ratiometric fluorescent sensor with different DNA-templated Ag NCs as signals for ATP detection. J Photochem Photobiol A Chem 400:112725–112731. https://doi.org/10.1016/j.jphotochem.2020.112725

    Article  CAS  Google Scholar 

  36. Zhang Z, Liu T, Wang S, Ma J, Zhou T, Wang F, Wang X, Zhang G (2019) DNA-templated gold nanocluster as a novel fluorometric sensor for glutathione determination. J Photochem Photobiol A Chem 370:89–93. https://doi.org/10.1016/j.jphotochem.2018.10.021

    Article  CAS  Google Scholar 

  37. Wang W, Li J, Fan J, Ning W, Liu B, Tong C (2018) Ultrasensitive and non-labeling fluorescence assay for biothiols using enhanced silver nanoclusters. Sens Actuators B Chem 267:174–180. https://doi.org/10.1016/j.snb.2018.04.010

    Article  CAS  Google Scholar 

  38. Liu Z, Wu Z, Yao Q, Cao Y, Chai OJH, Xie J (2021) Correlations between the fundamentals and applications of ultrasmall metal nanoclusters: Recent advances in catalysis and biomedical applications. Nano Today 36:101053–101075. https://doi.org/10.1016/j.nantod.2020.101053

    Article  CAS  Google Scholar 

  39. Xu X, Ray R, Gu Y, Ploehn HJ, Gearheart L, Raker K, Scrivens WA (2004) Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments. J Am Chem Soc 126:12736–12737. https://doi.org/10.1021/ja040082h

    Article  CAS  PubMed  Google Scholar 

  40. Sun Y, Zhou B, Lin Y, Wang W, Fernando KAS, Pathak P, Meziani MJ, Harruff BA, Wang X (2006) Quantum-sized carbon dots for bright and colorful photoluminescence. J Am Chem Soc 128:7756–7757. https://doi.org/10.1021/JA062677D

    Article  CAS  PubMed  Google Scholar 

  41. Huang G, Chen X, Wang C, Zheng H, Huang Z, Chen D, Xie H (2017) Photoluminescent carbon dots derived from sugarcane molasses: synthesis, properties, and applications. RSC Adv 7:47840–47847. https://doi.org/10.1039/C7RA09002A

    Article  CAS  Google Scholar 

  42. Wang R, Lu K, Tang Z, Xu Y (2017) Recent progress in carbon quantum dots: synthesis, properties and applications in photocatalysis. J Mater Chem A 5:3717–3734. https://doi.org/10.1039/C6TA08660H

    Article  CAS  Google Scholar 

  43. Yuan F, Li S, Fan Z, Meng X, Fan L, Yang S (2016) Shining carbon dots: Synthesis and biomedical and optoelectronic applications. Nano Today 11:565–586. https://doi.org/10.1016/j.nantod.2016.08.006

    Article  CAS  Google Scholar 

  44. Borse S, Jha S, Murthy ZVP, Kailasa SK (2022) Sustainable chemistry approach for the preparation of bluish green emissive copper nanoclusters from Justicia adhatoda leaves extract: a facile analytical approach for the sensing of myoglobin and l-thyroxine. New J Chem 46:15919–15928. https://doi.org/10.1039/d2nj02524h

    Article  CAS  Google Scholar 

  45. Chen J, Ran F, Chen Q, Luo D, Ma W, Han T, Wang C, Wang C (2019) A fluorescent biosensor for cardiac biomarker myoglobin detection based on carbon dots and deoxyribonuclease I-aided target recycling signal amplification. RSC Adv 9:4463–4468. https://doi.org/10.1039/C8RA09459D

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  46. Miao D, Liu D, Zeng Y, Zhou G, Xie W, Yang Y, Wang H, Zhang J, Zhai Y, Zhang Z, Li L (2020) Fluorescent aptasensor based on D-AMA/F-CSC for the sensitive and specific recognition of myoglobin. Spectrochim. Acta A Mol Biomol Spectrosc 228:117714–117719. https://doi.org/10.1016/j.saa.2019.117714

    Article  CAS  PubMed  Google Scholar 

  47. Wang Q, Yang X, Yang X, Liu F, Wang K (2015) Visual detection of myoglobin via G-quadruplex DNAzyme functionalized gold nanoparticles-based colorimetric biosensor. Sens Actuators B Chem 212:440–445. https://doi.org/10.1016/j.snb.2015.02.040

    Article  CAS  Google Scholar 

  48. Sullivan MV, Stockburn WJ, Hawes PC, Mercer T, Reddy SM (2021) Green synthesis as a simple and rapid route to protein modified magnetic nanoparticles for use in the development of a fluorometric molecularly imprinted polymer-based assay for detection of myoglobin. Nanotechnology 32:095502–095514. https://doi.org/10.1088/1361-6528/abce2d

    Article  CAS  PubMed  Google Scholar 

  49. Sun C, Wang D, Geng Z, Gao L, Zhang M, Bian H, Liu F, Zhu Y, Wu H, Xu W (2015) One-step green synthesis of a polypyrrole-Au nanocomposite and its application in myoglobin aptasensor. Anal Methods 7:5262–5268. https://doi.org/10.1039/c5ay01006c

    Article  CAS  Google Scholar 

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Funding

This work was supported by National Natural Science Key Foundation of China (Project no. 12032007), Chongqing Talents (cstc2022ycjh-bgzxm0166), Chongqing Talents Liao Xiao-ling (CQYC20210302379), Natural Science Foundation General Project of Chongqing in China (CSTC2019JCYJ-msxmX0221), Science and Technology Research Program of Chongqing Municipal Education Commission (KJQN202101537), Chongqing Technology Innovation and Application Development Project (CSTC 2019JSCX-msxmX0231), the Open Foundation of Chongqing Key Laboratory of Nano-micro Composites and Devices and Postgraduate Science and Technology Innovation Program of Chongqing University of Science and Technology (YKJCX1920205).

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Authors and Affiliations

  1. Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400030, China

    Zichen Xu & Guixue Wang

  2. Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing Engineering Laboratory of Nano/Micro Biomedical Detection, Chongqing University of Science and Technology, No. 12 East Road, University Town, Chongqing, 401331, People’s Republic of China

    Hedan Xu, Hongliang Duan, Junjie Li, Xiao Hu, Kaixin Jiang & Yuanyuan Zhang

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  1. Zichen Xu
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  2. Hedan Xu
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Contributions

All authors contributed to the conception and design of the research. Zichen Xu: Conceptualization, Methodology, Investigation, Writing-original draft, Review & editing. Hedan Xu: Methodology, Investigation, Writing-review & editing. Honglian Duan: Writing–review & editing. Junjie Li: Review & editing. Xiao Hu:Review & editing. Guixue Wang: Funding acquisition, Review & editing. Yuanyuan Zhang: Funding acquisition, Supervision, Review & editing.

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Correspondence to Guixue Wang or Yuanyuan Zhang.

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Xu, Z., Xu, H., Duan, H. et al. Smartphone-Aided Fluorescence Detection of Cardiac Biomarker Myoglobin by a Ratiometric Fluorescent AuNCs-QDs Nanohybrids Probe with High Sensitivity. J Fluoresc 34, 179–190 (2024). https://doi.org/10.1007/s10895-023-03246-8

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