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A Selective Fluorescent Nanoprobe Based on Graphene Quantum Dots and Hg2+ for the Determination of Tetracycline in Biological Samples

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Abstract

A simple, sensitive, and selective fluorometric method based on graphene quantum dots and Hg2+ is presented for the determination of tetracycline. The fluorescence emission of graphene quantum dots at 463 nm decreased in the presence of Hg2+ ions due to its electrostatic interaction with the negatively charged surface of quantum dots at pH = 8.0. The addition of tetracycline to this system resulted in the retrieval of the fluorescence emission of the graphene quantum dots proportional to the tetracycline concentration. This is because of the interaction between tetracycline and Hg2+ that results in the release of the quantum dots' surface. Under the optimized conditions, the calibration curve indicated good linearity in the range of 2.0–44.0 nmol L−1 with a detection limit of 0.52 nmol L−1 for tetracycline. The designed nanoprobe was capable of the determination of tetracycline in serum and urine samples.

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Data Availability

The datasets generated during and/or analyzed during the current study are not publicly available but are available from the corresponding author on reasonable request.”

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Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran

    Maryam Moallemi Bahmani, Ali Mohammad Haji Shabani, Shayessteh Dadfarnia & Roya Afsharipour

Authors
  1. Maryam Moallemi Bahmani
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  2. Ali Mohammad Haji Shabani
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  3. Shayessteh Dadfarnia
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  4. Roya Afsharipour
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Contributions

Maryam Moallemi Bahmani: Data curation, Investigation, Original draft preparation, Shayessteh Dadfarnia and Ali Mohammad Haji Shabani: Supervisor, Writing- reviewing and editing, Roya Afsharipour: Original draft preparation, Writing and conceptualization.

Corresponding authors

Correspondence to Ali Mohammad Haji Shabani or Shayessteh Dadfarnia.

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This is an observational study. The XYZ Research Ethics Committee has confirmed that no ethical approval is required.

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The authors declare that are no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service, and/or company.

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Moallemi Bahmani, M., Haji Shabani, A., Dadfarnia, S. et al. A Selective Fluorescent Nanoprobe Based on Graphene Quantum Dots and Hg2+ for the Determination of Tetracycline in Biological Samples. J Fluoresc 33, 1067–1075 (2023). https://doi.org/10.1007/s10895-022-03118-7

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