Skip to main content
SpringerLink
Log in

Imaging of Bacterial and Fungal Cells Using Fluorescent Carbon Dots Prepared from Carica papaya Juice

  • SHORT COMMUNICATION
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

In this paper, we have described a simple hydrothermal method for preparation of fluorescent carbon dots (C-dots) using Carica papaya juice as a precursor. The synthesized C-dots show emission peak at 461 nm with a quantum yield of 7.0 %. The biocompatible nature of C-dots was confirmed by a cytotoxicity assay on E. coli. The C-dots were used as fluorescent probes for imaging of bacterial (Bacillus subtilis) and fungal (Aspergillus aculeatus) cells and emitted green and red colors under different excitation wavelengths, which indicates that the C-dots can be used as a promising material for cell imaging.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

References

  1. Li Q, Liu L, Liu JW, Jiang JH, Yu RQ, Chu X (2014) Nanomaterial-based fluorescent probes for live-cell imaging. Trend Anal Chem 58:130–144

    Article  CAS  Google Scholar 

  2. Baker SN, Baker GA (2010) Luminescent carbon nanodots: emergent nanolights. Angew Chem Int Ed Engl 49:6726–6744

    Article  CAS  PubMed  Google Scholar 

  3. Li H, Kang Z, Liu Y, Lee ST (2012) Carbon nanodots: synthesis, properties and applications. J Mater Chem 22:24230–24253

    Article  CAS  Google Scholar 

  4. Sahu S, Behera B, Maiti T, Mohapatra S (2012) Simple one-step synthesis of highly luminescent carbon dots from orange juice: application as excellent bio-imaging agents. Chem Commun 48:8835–8837

    Article  CAS  Google Scholar 

  5. Liang Q, Ma W, Shi Y, Li Z, Yang X (2013) Easy synthesis of highly fluorescent carbon quantum dots from gelatin and their luminescent properties and applications. Carbon 60:421–428

    Article  CAS  Google Scholar 

  6. Li X, Wang H, Shimizu Y, Pyatenko A, Kawaguchia K, Koshizaki N (2011) Preparation of carbon quantum dots with tunable photoluminescence by rapid laser passivation in ordinary organic solvents. Chem Commun 47:932–934

    Article  Google Scholar 

  7. Mehta VN, Jha S, Kailasa SK (2014) One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells. Mater Sci Eng C 38:20–27

    Article  CAS  Google Scholar 

  8. Chowdhury D, Gogoia N, Majumdar G (2012) Fluorescent carbon dots obtained from chitosan gel. RSC Adv 2:12156–12159

    Article  CAS  Google Scholar 

  9. Jiang C, Wu H, Song X, Mab X, Wang J, Tan M (2014) Presence of photoluminescent carbon dots in Nescafe® original instant coffee: applications to bioimaging. Talanta 127:68–74

    Article  CAS  PubMed  Google Scholar 

  10. Liu H, Ye T, Mao C (2007) Fluorescent carbon nanoparticles derived from candle soot. Angew Chem Int Ed 46:6473–6475

    Article  CAS  Google Scholar 

  11. Zhou J, Sheng Z, Han H, Zou M, Li C (2012) Facile synthesis of fluorescent carbon dots using watermelon peel as a carbon source. Mater Lett 66:222–224

    Article  CAS  Google Scholar 

  12. Yin B, Deng J, Peng X, Long Q, Zhao J, Lu Q, Chen Q, Li H, Tang H, Zhang Y, Yao S (2013) Green synthesis of carbon dots with down- and up-conversion fluorescent properties for sensitive detection of hypochlorite with a dual-readout assay. Analyst 138:6551–6557

    Article  CAS  PubMed  Google Scholar 

  13. Lu W, Qin X, Liu S, Chang G, Zhang Y, Luo Y, Asiri AM, Al-Youbi AO, Sun X (2012) Anal Chem 84:5351–5357

    Article  CAS  PubMed  Google Scholar 

  14. Mewada A, Pandey S, Shinde S, Mishra N, Oza G, Thakur M, Sharon M, Sharon M (2013) Green synthesis of biocompatible carbon dots using aqueous extract of Trapa bispinosa peel. Mater Sci Eng C 33:2914–2917

    Article  CAS  Google Scholar 

  15. Teng X, Ma C, Ge C, Yan M, Yang J, Zhang Y, Moraiscd PC, Bi H (2014) Green synthesis of nitrogen-doped carbon dots from konjac flour with “off–on” fluorescence by Fe3+ and L-lysine for bioimaging. J Mater Chem B 2:4631–4639

    Article  CAS  Google Scholar 

  16. Liu Y, Zhao Y, Zhang Y (2014) One-step green synthesized fluorescent carbon nanodots from bamboo leaves for copper (II) ion detection. Sensors Actuators B 196:647–652

    Article  CAS  Google Scholar 

  17. Liu SS, Wang CF, Li CX, Wang J, Mao LH, Chen S (2014) Hair-derived carbon dots toward versatile multidimensional fluorescent materials. J Mater Chem C 2:6477–6483

    Article  CAS  Google Scholar 

  18. Wang L, Zhou HS (2014) Green synthesis of luminescent nitrogen-doped carbon dots from milk and its imaging application. Anal Chem 86:8902–8905

    Article  CAS  PubMed  Google Scholar 

  19. Nie H, Li M, Li Q, Liang S, Tan Y, Sheng L, Shi W, Xiao-An Zhang S (2014) Carbon dots with continuously tunable full-color emission and their application in ratiometric pH sensing. Chem Mater 26:3104–3112

    Article  CAS  Google Scholar 

  20. Park SY, Lee HU, Park ES, Lee SC, Lee JW, Jeong SW, Kim CH, Lee YC, Huh YS, Lee J, Green P (2014) Photoluminescent green carbon nanodots from food-waste-derived sources: large-scale synthesis, properties, and biomedical applications. ACS Appl Mater Interfaces 6:3365–3370

    Article  CAS  PubMed  Google Scholar 

  21. Zhai Y, Zhu Z, Zhu C, Ren J, Wang E, Dong S (2014) Multifunctional water-soluble luminescent carbon dots for imaging and Hg2+ sensing. J Mater Chem B 2:6995–6999

    Article  CAS  Google Scholar 

  22. Du F, Yuan J, Zhang M, Li J, Zhou Z, Li Z, Cao M, Chen J, Zhang L, Liu X, Gong A, Xua W, Shao Q (2014) Nitrogen-doped carbon dots with heterogeneous multi-layered structures. RSC Adv 4:37536–37541

    Article  CAS  Google Scholar 

  23. Mora IC, Romero V, Lavilla I, Bendicho C (2014) In situ building of a nanoprobe based on fluorescent carbon dots for methylmercury detection. Anal Chem 86:4536–4543

    Article  Google Scholar 

  24. Li CL, Ou CM, Huang CC, Wu WC, Chen YP, Lin TE, Ho LC, Wang CW, Shih CC, Zhou HC, Lee YC, Tzeng WF, Chiou TJ, Chu ST, Cangm J, Chang HT (2014) Carbon dots prepared from ginger exhibiting efficient inhibition of human hepatocellular carcinoma cells. J Mater Chem B 2:4564–4571

    Article  CAS  Google Scholar 

  25. Wu L, Cai X, Nelson K, Xing W, Xia J, Zhang R, Stacy AJ, Luderer M, Lanza GM, Wang LV, Shen B, Pan D (2013) A green synthesis of carbon nanoparticle from honey for real-time photoacoustic imaging. Nano Res 6:312–325

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  26. Prasannan A, Imae T (2013) One-pot synthesis of fluorescent carbon dots from orange waste peels. Ind Eng Chem Res 52:15673–15678

    Article  CAS  Google Scholar 

  27. Qiao ZA, Wang Y, Gao Y, Li H, Dai T, Liu Y, Huo Q (2010) Commercially activated carbon as the source for producing multicolor photoluminescent carbon dots by chemical oxidation. Chem Commun 46:8812–8814

    Article  CAS  Google Scholar 

  28. Sambrook J, Fritsch EF, Maniatis T (1989) Cold Spring Harbor, New York

  29. Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer Academic/Plenum Publishers, New York

    Book  Google Scholar 

  30. Mehta VN, Jha S, Singhal RK, Kailasa SK (2014) Preparation of multicolor emitting carbon dots for HeLa cell imaging. New J Chem 38:6152–6160

    Article  CAS  Google Scholar 

  31. Zhu W, Zhang J, Jiang Z, Wang W, Liu X (2014) High-quality carbon dots: synthesis, peroxidase-like activity and their application in the detection of H2O2, Ag+ and Fe3+. RSC Adv 4:17387–17392

    Article  CAS  Google Scholar 

  32. Smith AM, Nie S (2011) Bright and compact alloyed quantum dots with broadly tunable near-infrared absorption and fluorescence spectra through mercury cation exchange. J Am Chem Soc 133:24–26

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  33. Niu J, Gao H (2014) Synthesis and drug detection performance of nitrogen-doped carbon dots. J Lumin 149:159–162

    Article  CAS  Google Scholar 

  34. Leis J, Perkson A, Arulepp M, Kaarik M, Svensson G (2001) Carbon nanostructures produced by chlorinating aluminium carbide. Carbon 39:2043–2048

    Article  CAS  Google Scholar 

  35. Yan F, Zou Y, Wang M, Mu X, Yang N, Chen L (2014) Highly photoluminescent carbon dots-based fluorescent chemosensors for sensitive and selective detection ofmercury ions and application of imaging in living cells. Sensors Actuators B 192:488–495

    Article  CAS  Google Scholar 

  36. Liu J, Liu X, Luoab H, Gao Y (2014) One-step preparation of nitrogen-doped and surface-passivated carbon quantum dots with high quantum yield and excellent optical properties. RSC Adv 4:7648–7654

    Article  CAS  Google Scholar 

  37. Nanninga N (1998) Morphogenesis of Escherichia coli. Microbiol Mol Biol Rev 62:110–129

    CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the S. V. National Institute of Technology, Surat under M.Sc., research project. We also thank Department of Science and Technology for providing Maya Pro 2000 spectrophotometer under the Fast-Track Young Scientist Scheme (2011 – 2014).

Author information

Author notes

    Authors and Affiliations

    1. Department of Applied Chemistry, S. V. National Institute of Technology, Surat, 395 007, India

      Betha Saineelima B. Kasibabu, Stephanie L. D’souza & Suresh Kumar Kailasa

    2. Gujarat Agricultural Biotechnology Institute, Navsari Agricultural University, Surat, 395007, India

      Sanjay Jha

    Authors
    1. Betha Saineelima B. Kasibabu
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Stephanie L. D’souza
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Sanjay Jha
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Suresh Kumar Kailasa
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Suresh Kumar Kailasa.

    Additional information

    Betha Saineelima B. Kasibabu and Stephanie L. D’souza contributed equally to this work.

    Electronic supplementary material

    Below is the link to the electronic supplementary material.

    ESM 1

    (PPT 332 kb)

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Kasibabu, B.S.B., D’souza, S.L., Jha, S. et al. Imaging of Bacterial and Fungal Cells Using Fluorescent Carbon Dots Prepared from Carica papaya Juice. J Fluoresc 25, 803–810 (2015). https://doi.org/10.1007/s10895-015-1595-0

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s10895-015-1595-0

    Keywords

    Search

    Navigation