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A Simple and Sensitive Fluorescence Quenching Method for the Determination of H2O2 Using Rhodamine B and Fe3O4 Nanocatalyst

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Abstract

In pH 1.99 sodium acetate-HCl buffer solutions at 60 °C, Rhodamine B exhibited a strong fluorescence peak at 584 nm using an excitation wavelength of 548 nm. The fluorescence quenching occurred when Fe3O4 nanoparticles catalyzed H2O2 oxidation of Rhodamine B. Under the chosen conditions, the fluorescence intensity at 584 nm decreased when the concentration of H2O2 increased. The fluorescence quenching intensity is linear with the concentration of H2O2 in the range of 10–200 nmol/L. Thus, a new and simple and sensitive nanocatalytic fluorescence method was proposed for the determination of H2O2 in synthetic sample, with satisfactory results.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 20865002, 20965002, 21075023), Natural Science Foundation of Guangxi (No.0991021Z) and the Research Funds of Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment (No. 0701Z022).

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Authors and Affiliations

  1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Conservation of Education Ministry, Guangxi Key Laboratory of Environmental Engineering and Protection and Assessment, Guangxi Normal University, Guilin, 541004, China

    Zhiliang Jiang, Li Kun, Huixiang Ouyang & Aihui Liang

  2. Animal Science and Technology Collage, Guangxi University, Nanning, 531004, China

    Hesheng Jiang

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  1. Zhiliang Jiang
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  2. Li Kun
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  3. Huixiang Ouyang
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  4. Aihui Liang
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  5. Hesheng Jiang
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Correspondence to Zhiliang Jiang or Hesheng Jiang.

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Jiang, Z., Kun, L., Ouyang, H. et al. A Simple and Sensitive Fluorescence Quenching Method for the Determination of H2O2 Using Rhodamine B and Fe3O4 Nanocatalyst. J Fluoresc 21, 2015–2020 (2011). https://doi.org/10.1007/s10895-011-0902-7

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  • DOI: https://doi.org/10.1007/s10895-011-0902-7

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