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An Easy and Efficient Fluorescent Method for Detecting Aldehydes and Its Application in Biotransformation

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Abstract

Water-soluble aldehydes (acetaldehyde, propionaldehyde) and non-water-soluble aldehydes (butyraldehyde and phenylacetaldehyde) were easily detected by an efficient fluorescent method with 5-aminofluorescein as probe. Under optimal detection conditions, 5-aminofluorescein could selectively respond to aldehydes with high sensitivity in comparison with other carbonyl compounds like ketones and acids. Thus, the proposed method was used to monitor microbial oxidation and succeeded in trapping transiently-produced aldehydes during biotransformation of primary alcohols by Gluconobacter oxydans.

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Acknowledgements

This work was supported by the National Basic Research Program of China (973 program, No. 2009CB724703).

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Author notes

  1. Xiangzhao Mao

    Present address: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China

Authors and Affiliations

  1. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, People’s Republic of China

    Yanlong Xing, Shu Wang, Xiangzhao Mao & Xuebo Zhao

  2. Newworld Institute of Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Dongzhi Wei

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  1. Yanlong Xing
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  2. Shu Wang
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  3. Xiangzhao Mao
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  4. Xuebo Zhao
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  5. Dongzhi Wei
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Correspondence to Dongzhi Wei.

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Xing, Y., Wang, S., Mao, X. et al. An Easy and Efficient Fluorescent Method for Detecting Aldehydes and Its Application in Biotransformation. J Fluoresc 21, 587–594 (2011). https://doi.org/10.1007/s10895-010-0746-6

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  • DOI: https://doi.org/10.1007/s10895-010-0746-6

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