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Colored and White Sectors From Star-Patterned Petunia Flowers Display Differential Resistance to Corn Earworm and Cabbage Looper Larvae

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Abstract

Anthocyanins are likely a visual aid that attract pollinators. However, there is also the possibility that anthocyanins are present in some flowers as defensive molecules that protect them from excess light, pathogens, or herbivores. In this study, resistance due to anthocyanins from commercial petunia flowers (Petunia hybrida) was examined for insecticide/antifeedant activity against corn earworm (CEW, Helicoverpa zea) and cabbage looper (CL, Trichoplusia ni). The petunia flowers studied contained a star pattern, with colored and white sectors. CEW larvae ate significantly less colored sectors than white sectors in no-choice bioassays in most cases. All CEW larvae feeding on blue sectors weighed significantly less after 2 days than larvae feeding on white sectors, which was negatively correlated with total anthocyanin levels. CL larvae ate less of blue sectors than white sectors, and blue sectors from one petunia cultivar caused significantly higher CL mortality than white sectors. Partially purified anthocyanin mixtures isolated from petunia flowers, when added to insect diet discs at approximately natural concentrations, reduced both CEW and CL larva weights compared to the controls. These studies demonstrate that the colored sectors of these petunia cultivars slow the development of these lepidopteran larvae and indicate that anthocyanins play some part in flower defense in petunia.

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Acknowledgment

We thank David Lee, Kay Shopinski, Ray Holloway, Sandi Duval, Tessa DeYoung, and Emily Bell for technical assistance. We are grateful to Bob Behle, Allard Cossé, and Brent Tisserat for comments on prior versions of this manuscript.

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Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture.

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Correspondence to Eric T. Johnson.

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Johnson, E.T., Berhow, M.A. & Dowd, P.F. Colored and White Sectors From Star-Patterned Petunia Flowers Display Differential Resistance to Corn Earworm and Cabbage Looper Larvae. J Chem Ecol 34, 757–765 (2008). https://doi.org/10.1007/s10886-008-9444-0

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  • DOI: https://doi.org/10.1007/s10886-008-9444-0

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