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Quantification of Invertase Activity in Ants Under Field Conditions

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Abstract

Invertases (EC 3.2.1.26) are hydrolases that cleave sucrose into the monosacccharides, glucose, and fructose. They play a central role in carbohydrate metabolism of plants and animals. Methods presented so far to quantify invertase activity in ants or other animals have been hampered by the variability in both substrates and products of the enzymatic reaction in animals whose carbohydrate metabolism is highly active. Our method is based on a spectrophotometric quantification of the kinetics of glucose release. We first obtained an equilibrium state summarizing reactions of any carbohydrates and enzymes that are present in the extract. Sucrose was then added to quantify invertase activity as newly released glucose. Invertase activities differed significantly among species of ants. Variances were lowest among individuals from the same colony and highest among different species. When preparations were made from ants of the same species, invertase activity was linearly related to the number of ants used for extraction. Our method does not require ants to be kept on specific substrates prior to the experiment, or expensive or large equipment. It, thus, appears suitable for dealing with a broad range of physiological, ecological, and evolutionary questions.

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References

  • Ayre, G. L. 1963. Feeding behaviour and digestion in Camponotus herculeanus (L.) (Hymenoptera: Formicidae). Entomol. Exp. Appl. 6:165–170.

    Google Scholar 

  • Ayre, G. L. 1967. The relationships between food and digestive enzymes in five species of ants (Hymenoptera: Formicidae). Can. Entomol. 99:408–411.

    Google Scholar 

  • Boevé, J. L. and Wäckers, F. L. 2003. Gustatory perception and metabolic utilization of sugars by Myrmica rubra ant workers. Oecologia 136:508–514.

    Google Scholar 

  • Heil, M. and McKey, D. 2003. Protective ant-plant interactions as model systems in ecological and evolutionary research. Annu. Rev. Ecol. Evol. Syst. 34:425–453.

    Google Scholar 

  • Henrissat, B. and Bairoch, A. 1993. New families in the classification of glycosyl hydrolases based on amino-acid-sequence similarities. Biochem. J. 293:781–788.

    Google Scholar 

  • Martínez del Rio, C. 1990. Dietary, phylogenetic, and ecological correlates of intestinal sucrase and maltase activity in birds. Physiol. Zool. 63:987–1011.

    Google Scholar 

  • D. G. Naumoff, 2001. β -Fructosidase superfamily: Homology with some α -l-arabinases and β -d-xylosidases. Proteins Struct. Funct. Genet. 42:66–76.

    Google Scholar 

  • B. L. Ricks, S. B. Vinson, 1972. Digestive enzymes of imported fire ant, Solenopsis richteri (Hymenoptera: Formicidae). Entomol. Exp. Appl. 15:329–334.

    Google Scholar 

  • A. Sturm, G. Q. Tang, 1999. The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. Trends Plant Sci. 4:401–407.

    Google Scholar 

  • Ricks, B. L. and Vinson, S. B. 1972. Digestive enzymes of imported fire ant, Solenopsis richteri (Hymenoptera: Formicidae). Entomol. Exp. Appl. 15:329–334.

    Google Scholar 

  • Sturm, A. and Tang, G. Q. 1999. The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitioning. Trends Plant Sci. 4:401–407.

    Google Scholar 

  • Zhang, J., Scrivener, A. M., Slaytor, M., and Rose, H. A. 1993. Diet and carbohydrase activities in three cockroaches, Calolampra elegans Roth and Princis, Geoscapheus dilatatus Saussure and Panesthia cribrata Saussure. Comp. Biochem. Physiol. A 104:155–161.

    Google Scholar 

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Correspondence to Martin Heil.

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Heil, M., BÜchler, R. & Boland, W. Quantification of Invertase Activity in Ants Under Field Conditions. J Chem Ecol 31, 431–437 (2005). https://doi.org/10.1007/s10886-005-1352-y

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  • DOI: https://doi.org/10.1007/s10886-005-1352-y

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