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Seagrasses as Sources of Mosquito Nano-Larvicides? Toxicity and Uptake of Halodule uninervis-Biofabricated Silver Nanoparticles in Dengue and Zika Virus Vector Aedes aegypti

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Abstract

Mosquitoes (Diptera: Culicidae) act as vectors for devastating pathogens and parasites. Zika virus, an Aedes mosquito-borne flavivirus, is becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly in the infants of some women who were pregnant when they contracted the disease. There are no specific treatments for Zika virus, thus the eco-friendly and effective control of mosquito vectors is crucial. This research proposed a novel method of seagrass-mediated synthesis of silver nanoparticles (AgNP) using Halodule uninervis as a reducing and capping agent. UV–Vis spectroscopy, FTIR spectroscopy, SEM, EDX spectroscopy, XRD and Raman analysis confirmed the rapid and cheap synthesis of AgNP. LC50 of H. uninervis extract against Aedes aegypti was 295.629 ppm and LC50 of H. uninervis-synthesized AgNP was 12.554 ppm. Microscopy analysis pointed out the uptake of H. uninervis-fabricated AgNP in the midgut of mosquito larvae. In MIC assays, low doses of the AgNP inhibited the growth of Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi. Overall, this research shed light on the mosquitocidal potential of H. uninervis, as a bio-resource for the cheap and effective nanosynthesis of mosquitocidal biopesticides.

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Acknowledgements

Dr. Jazem A. Mahyoub is grateful to the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, Grant No. G-256-130-37. The authors would like to extend their sincere appreciation to the Deanship of Scientific Research for its technical and financial support.

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Authors and Affiliations

  1. Department of Biology Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

    Jazem A. Mahyoub

  2. IBB University, Ibb, Republic of Yemen

    Jazem A. Mahyoub

  3. Biology Department, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia

    Al Thabiani Aziz, Chellasamy Panneerselvam & Subrata Trivedi

  4. Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, Tamil Nadu, 641046, India

    Kadarkarai Murugan & Mathath Roni

  5. Thiruvalluvar University, Serkkadu, Vellore, Tamil Nadu, 632 115, India

    Kadarkarai Murugan

  6. Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Marcello Nicoletti

  7. Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

    Usama W. Hawas, Fekri M. Shaher & Muneer A. Bamakhrama

  8. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    Angelo Canale & Giovanni Benelli

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  1. Jazem A. Mahyoub
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  2. Al Thabiani Aziz
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Mahyoub, J.A., Aziz, A.T., Panneerselvam, C. et al. Seagrasses as Sources of Mosquito Nano-Larvicides? Toxicity and Uptake of Halodule uninervis-Biofabricated Silver Nanoparticles in Dengue and Zika Virus Vector Aedes aegypti . J Clust Sci 28, 565–580 (2017). https://doi.org/10.1007/s10876-016-1127-3

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