Skip to main content
SpringerLink
Log in

Green-Synthesis of Selenium Nanoparticles Using Garlic Cloves (Allium sativum): Biophysical Characterization and Cytotoxicity on Vero Cells

  • Original Paper
  • Published:
Journal of Cluster Science Aims and scope Submit manuscript

Abstract

Nanotechnology has the potential to revolutionize a number of research fields, including biomedicine. Currently, the fabrication of biocompatible nanomaterials is of high interest worldwide. Little is known about the possible synthetic routes to fabricate selenium nanoparticles. In this research, we focused on the potential of a medicinally important plant species, garlic (Allium sativum), for the green synthesis of selenium nanoparticles. The garlic aqueous extracts acted as a capping and reducing agent in forming selenium nanoparticles. Green-synthesized selenium nanoparticles were characterized using UV-vis spectrophotometry, TEM, SEM, EDAX, FTIR and XRD analysis. Furthermore, green-fabricated and conventional chemically synthesized selenium nanoparticles were tested to evaluate their cytotoxicity against Vero cells. CC50 values revealed that biologically synthesized selenium nanoparticles showed eco-friendly features and limited cytotoxicity if compared with chemically synthesized selenium nanoparticles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. R. Nagarajan and T. Alan Hatton (2008). Nanoparticles: Building Blocks for Nanotechnology: Synthesis, Stabilization, Passivation, and Functionalization. ACS Symposium Series; ACS: Washington, DC.

  2. A. Ramanavicius, A. Kausaite, and A. Ramanaviciene (2005). Biosens. Bioelectron. 20, 1962.

    Article  CAS  Google Scholar 

  3. A. Mohan (2015). IJERMT 4, 23.

    Google Scholar 

  4. Y. Gao, J. K. Shen, L. Milane, F. J. Hornicek, M. M. Amiji, and Z. Duan (2015). Curr. Med. Chem. 22, 1335.

    Article  CAS  Google Scholar 

  5. J. Nakanishi, T. Takarada, K. Yamaguchi, and M. Maeda (2008). Anal. Sci. 24, 67.

    Article  CAS  Google Scholar 

  6. V. Y. T. Ho, B. Poinard, and J. C. Kah (2016). Nanomedicine 6, 693.

    Article  Google Scholar 

  7. L. Kang, Z. Gao, W. Huang, M. Jin, and Q. Wang (2015). Acta. Pharm. Sin. B 3, 169.

    Article  Google Scholar 

  8. M. Venkatachalam, G. Singaravelu, K. Govindaraju, and J. SeogAhn (2013). Curr. Sci. 105, 827.

    CAS  Google Scholar 

  9. H. Michael, L. G. Alan, and C. Serge (2014). Front. Chem. 2, 63.

    Google Scholar 

  10. G. Benelli (2016). Parasitol. Res. 115, 23.

    Article  Google Scholar 

  11. R. Guo, Y. Song, G. Wang, and R. W. Murray (2005). J. Am. Chem. Soc. 127, 2752.

    Article  CAS  Google Scholar 

  12. M. C. Daniel and D. Astruc (2004). Chem. Rev. 104, 293.

    Article  CAS  Google Scholar 

  13. C. C. Huang, Z. Yang, K. H. Lee, and H. T. Chang (2007). Angew. Chem. Int. Ed. 46, 6824.

    Article  CAS  Google Scholar 

  14. C. Jensen and J. Pallauf (2008). J. Anim. Physiol. Anim. Nutr. 92, 481.

    Article  CAS  Google Scholar 

  15. M. P. Rayman (2000). Lancet 356, 233.

    Article  CAS  Google Scholar 

  16. A. Abdelouas, W. L. Gong, W. Lutze, J. A. Shelnutt, R. Franco, and I. Moura (2000). Chem. Mater. 12, 1510.

    Article  CAS  Google Scholar 

  17. B. Gates, B. Mayers, B. Cattle, and Y. Xia (2002). Adv. Funct. Mater. 12, 219.

    Article  CAS  Google Scholar 

  18. J. Finley (1998). J. Agric. Food Chem. 46, 3702.

    Article  CAS  Google Scholar 

  19. P. Visha, K. Nanjappan, P. Selvaraj, S. Jayachandran, A. Elango, and G. Kumaresan (2015). Int. J. Adv. Vet. Sci. Technol. 4, 178.

    Google Scholar 

  20. J. M. Song, J. H. Zhu, and S. H. Yu (2006). J. Phys. Chem. B 110, 23790.

    Article  CAS  Google Scholar 

  21. Q. Xie, Z. Dai, W. W. Huang, W. Zhang, D. K. Ma, X. K. Hu, and Y. T. Qian (2006). Cryst. Growth Des. 6, 1514.

    Article  CAS  Google Scholar 

  22. M. Quintana, E. Haro-Poniatowski, J. Morales, and N. Batina (2002). Appl. Surf. Sci. 195, 175.

    Article  CAS  Google Scholar 

  23. E. Filippo, D. Manno, and A. Serra (2010). Cryst. Growth Des. 10, 4890.

    Article  CAS  Google Scholar 

  24. L. Shi, W. Xun, W. Yue, C. Zhang, Y. Ren, L. Shi, Q. Wang, R. Yang, and F. Lei (2011). Small Rumin. Res. 96, 49.

    Article  Google Scholar 

  25. C. Rathgeber, N. Yurkova, E. Stackebrandt, J. T. Beatty, and V. Yurkov (2002). Appl. Environ. Microbiol. 68, 4613.

    Article  CAS  Google Scholar 

  26. M. Morita, H. Uemoto, and A. Watanabe (2007). Eng. Life Sci. 7, 235.

    Article  CAS  Google Scholar 

  27. A. Klonowska, T. Heulin, and A. Vermeglio (2005). Appl. Environ. Microbiol. 71, 5607.

    Article  CAS  Google Scholar 

  28. B. Zare, S. Babaie, N. Setayesh, and A. R. Shahverdi (2013). Nanomed. J. 1, 13.

    Google Scholar 

  29. P. J. Fesharaki, P. Nazari, M. Shakibaie, S. Rezaie, M. Banoee, M. Abdollahi, and A. R. Shahverdi (2010). Braz. J. Microbiol. 41, 461.

    Article  CAS  Google Scholar 

  30. W. Zhang, Z. Chen, H. Liu, L. Zhang, P. Gaoa, and D. Li (2011). Colloids Surf. B 88, 196.

    Article  CAS  Google Scholar 

  31. F. Yang, Q. Tang, X. Zhong, Y. Bai, T. Chen, Y. Zhang, Y. Li, and W. Zheng (2012). Int. J. Nanomed. 7, 835.

    Article  CAS  Google Scholar 

  32. T. Chen, Y. S. Wong, W. Zheng, Y. Bai, and L. Huang (2008). Colloids Surf. B 67, 26.

    Article  CAS  Google Scholar 

  33. G. Sharma, A. R. Sharma, R. Bhavesh, J. Park, B. Ganbold, J. S. Nam, and S. S. Lee (2014). Molecules 19, 2761.

    Article  Google Scholar 

  34. S. K. Li, Y. H. Shen, A. J. Xie, X. R. Yu, X. Z. Zhang, L. B. Yang, and C. H. Li (2007). Nanotechnology 18, 405101.

    Article  Google Scholar 

  35. K. S. Prasad and K. Selvaraj (2014). Biol. Trace Elem. Res. 157, 275.

    Article  CAS  Google Scholar 

  36. C. Ramamurthy, K. S. Sampath, P. Arunkumar, M. S. Kumar, V. Sujatha, K. Premkumar, and C. Thirunavukkarasu (2013). Bioprocess Biosyst. Eng. 36, 1131.

    Article  CAS  Google Scholar 

  37. H. Kong, J. Yang, Y. Zhang, Y. Fang, K. Nishinari, and G. O. Phillips (2014). Int. J. Biol. Macromol. 65, 155.

    Article  CAS  Google Scholar 

  38. K. S. Prasad, H. Patel, T. Patel, K. Patel, and K. Selvaraj (2013). Colloids Surf. B 103, 261.

    Article  CAS  Google Scholar 

  39. S. Ankri and D. Mirelman (1999). Microbes Infect. 2, 125.

    Article  Google Scholar 

  40. C. R. Nwokocha, R. I. Ozolua, D. U. Owu, M. I. Nwokocha, and A. C. Ugwu (2011). Niger. J. Physiol. Sci. 26, 213.

    CAS  Google Scholar 

  41. N. Gupta and T. D. Porter (2001). J. Nutr. 131, 1662.

    CAS  Google Scholar 

  42. C. T. Liu, L. Y. Sheen, and C. K. Lii (2007). Mol. Nutr. Food Res. 51, 1353.

    Article  CAS  Google Scholar 

  43. A. Tsubura, Y. C. Lai, M. Kuwata, N. Uehara, and K. Yoshizawa (2011). Anticancer Agents Med. Chem. 3, 249.

    Article  Google Scholar 

  44. B. S. Kendler (1987). Prev. Med. 5, 670.

    Article  Google Scholar 

  45. K. Vinaykumar The Nervous System. in V. kumar and L. Stanley (eds.), Robbins Basic Pathology, 7th ed (Saunders, Philadelphia, 2003), p. 841.

    Google Scholar 

  46. R. Qi, F. Liao, E. Inoue, Y. Yatomi, K. Sato, and Y. Ozaki (2000). Biochem. Pharmacol. 60, 1475.

    Article  CAS  Google Scholar 

  47. D. charu, P. S. Chetan, S. Krishnankant, K. Manmohan, and B. Parma (2011). J. Nanosci. Nanotechnol. 1155, 1.

    Google Scholar 

  48. T. Mosmann (1983). J. Immunol. Methods 65, 55.

    Article  CAS  Google Scholar 

  49. Z. H. Lin and C. R. C. Wang (2005). Mater. Chem. Phys. 92, 591.

    Article  CAS  Google Scholar 

  50. H. Chen, Y. Ji-Beom, Y. Liu, and G. Zhao (2011). Electron Mater. Lett. 7, 333.

    Article  CAS  Google Scholar 

  51. A. R. Ingole, S. R. Thakare, N. T. Khati, A. V. Wankhadea, and D. K. Burghate (2010). Chalcogenide Lett. 7, 485.

    CAS  Google Scholar 

  52. R. R. Mishra, S. Prajapati, J. Das, T. K. Dangar, N. Das, and H. Thatoi (2011). Chemosphere 84, 1231.

    Article  CAS  Google Scholar 

  53. Y. Shen, W. Xiufang, A. Xie, L. Huang, J. Zhu, and L. Chen (2008). Mater. Chem. Phys. 109, 534.

    Article  CAS  Google Scholar 

  54. T. Ashokkumar, D. Prabhu, R. Geetha, K. Govindaraju, R. Manikandan, C. Arulvasu, and G. Singaravelu (2014). Colloids Surf. B 123, 549.

    Article  CAS  Google Scholar 

  55. R. Geetha, T. Ashokkumar, S. Tamilselvan, K. Govindaraju, A. M. Sadiq, and G. Singaravelu (2011). Cancer Nanotechnol. 4, 91.

    Article  Google Scholar 

  56. R. Lei, M. Yi, Z. Manjiao, L. Tianjie, W. Yayu, and H. An (2014). Int. J. Nanomed. 9, 4819.

    Google Scholar 

  57. A. Simon-Deckers, E. Brun, B. Gouget, M. Carrière, and C. Sicard-Roselli (2008). Gold Bull. 41, 187.

    Article  CAS  Google Scholar 

  58. J. H. Fan, W. I. Hung, W. T. Li, and J. M. Yeh (2008). Biocompatibility Study of Gold Nanoparticles to Human Cells. in C. T. Lim and J. C. H. Goh (eds.), ICBME, Proceedings 23, 870.

  59. A. K. Salem, P. C. Searson, and K. W. Leong (2003). Nat. Mater. 2, 668.

    Article  CAS  Google Scholar 

  60. R. Kesarkar, S. Shroff, M. Yeole, and A. Chowdhary (2015). JOJ Immunol. Virol. 1, 555552.

    Google Scholar 

  61. P. Orlowski, E. Tomaszewska, M. Gniadek, P. Baska, J. Nowakowska, and J. Sokolowska (2014). PLoS ONE 9, e104113.

    Article  Google Scholar 

  62. F. Sambale, S. Wagner, F. Stahl, R. R. Khaydarov, T. Scheper, and D. Bahnemann (2015). J. Nanomater. doi:10.1155/2015/136765.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Nanoscience Division, Department of Zoology, Thiruvalluvar University, Vellore, 632 115, India

    Kasi Anu, Ganesan Singaravelu & Kadarkarai Murugan

  2. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

Authors
  1. Kasi Anu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ganesan Singaravelu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kadarkarai Murugan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Giovanni Benelli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ganesan Singaravelu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Anu, K., Singaravelu, G., Murugan, K. et al. Green-Synthesis of Selenium Nanoparticles Using Garlic Cloves (Allium sativum): Biophysical Characterization and Cytotoxicity on Vero Cells. J Clust Sci 28, 551–563 (2017). https://doi.org/10.1007/s10876-016-1123-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10876-016-1123-7

Keywords

Search

Navigation