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Analysis of CD28 and bcl-2 Expression on Peripheral Blood and Liver-Infiltrating Mononuclear Cells in Patients with Autoimmune Hepatitis

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Abstract

Because the underlying mechanism of hepatocellular damages in autoimmune hepatitis (AIH) still remains unclear, analysis of CD28 and bcl-2 molecules, which are critical for T cell activation and survival, was performed in patients with AIH. The number of CD28(+)CD4(+) peripheral blood mononuclear cells (PBMC) in corticosteroid (CS)-treated patients was comparable to normal control individuals but decreased in untreated AIH patients. In contrast, the number of CD28(+)CD8(+) PBMC was decreased in both CS-treated and untreated AIH patients. Analysis of liver-infiltrating mononuclear cells (LIMC) showed that the number of CD28(+)CD4(+) and CD28(−)CD8(+) LIMC were positively correlated with the histology activity index score. Bcl-2(+)CD4(+) LIMC were observed in the portal area of the liver and the numbers fluctuated with disease activity during the time course after CS administration. By contrast, CD8(+) LIMC were shown not to express bcl-2. Taken collectively, these results suggest that bcl-2(+)CD28(+)CD4(+) and bcl-2(−)CD28(−)CD8(+) cells may play critical and distinct roles in hepatocellular damage in AIH.

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ACKNOWLEDGMENTS

We are grateful to Dr. Graham Leggatt, Center Biology Program, CICR, R-Wing, Princess Alexandra Hospital, Australia for his critical reading of the manuscript and helpful suggestions and Ms. Aya Iwai, KKR Takamatsu Hospital, Japan for her helpful technical support for making up the manuscripts.

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Authors and Affiliations

  1. Department of Gastroenterology and Neurology, Kagawa University School of Medicine, Miki-cho, Kagawa, Japan

    KAZUTAKA KUROKOHCHI, KEIJI ARIMA, TSUTOMU MASAKI, AKIIHIRO DEGUCHI, SEIJI NAKAI, ASAHIRO MORISHITA, HIROHITO YONEYAMA, TOMOHIRO OHGI, MASAHIRO ONO, AKIRA YOSHITAKE, MIKIO NISHIOKA & SHIGEKI KURIYAMA

  2. Department of Gastroenterology, KKR Takamatsu Hospital, Miki-cho, Kagawa, Japan

    KAZUTAKA KUROKOHCHI, TOMOHIRO OHGI, MASAHIRO ONO, AKIRA YOSHITAKE, TSUYOSHI MAETA, YOSHIHIRO MORI & FUMIKAZU KOHI

  3. Department of Gastroenterology and Neurology, Kagawa University School of Medicine, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa, 761-0793, Japan

    SHIGEKI KURIYAMA

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  1. KAZUTAKA KUROKOHCHI
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KUROKOHCHI, K., ARIMA, K., MASAKI, T. et al. Analysis of CD28 and bcl-2 Expression on Peripheral Blood and Liver-Infiltrating Mononuclear Cells in Patients with Autoimmune Hepatitis. J Clin Immunol 26, 323–330 (2006). https://doi.org/10.1007/s10875-006-9030-6

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  • DOI: https://doi.org/10.1007/s10875-006-9030-6

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