Abstract
Circular RNAs (circRNAs) showing unusual expressions have been discovered in pancreatic adenocarcinoma (PAAD). However, the functions and underlying mechanisms of these circRNAs still remain largely unclear. Our current study discovered a notable increase in the expression of circRNA hsa_circ_0002395 (circ_0002395) in both PAAD tissues and cell lines. This up-regulation of circ_0002395 was found to be associated with larger tumor sizes and lymph node metastasis. Furthermore, our findings showed that circ_0002395 facilitated aerobic glycolysis and cell proliferation in PAAD cells by regulating the miR-548c-3p/PDK1 axis. Mechanistically, we identified circ_0002395 as a competing endogenous RNA (ceRNA) that sponged miR-548c-3p, thereby promoting PDK1 expression and aerobic glycolysis, and ultimately resulting in the enhancement of cell proliferation. Our findings found that circ_0002395 promoted proliferation of PAAD cells by enhancing PDK1 expression and aerobic glycolysis by sponging miR-548c-3p.
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The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Funding
This work was supported by the Zhejiang Provincial Natural Science Foundation of China (No.LTGY23H200001), the Zhejiang Province Public Welfare Technology Application Research Project (No.LGF22H160015), the National College Students' Innovation and Entrepreneurship Training Program of China (No.202310350055; 202310350057), and the Taizhou Municipal Science and Technology Bureau (No.22ywa59).
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Meng-lu Shu (MS) and Jun-kai Xia (JX) drafted the manuscript, and contributed to the analysis and interpretation of data. Jing Yan (JY), Yu-jie Feng (YF) and Xiao-sheng Teng (XT) contributed in the data collection. Cui-juan Qian (CQ), Xiao-sheng Teng (XT), and Jun Yao (JY) conceived and designed the experiments. All authors read and approved the final manuscript.
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Shu, Ml., Xia, Jk., Yan, J. et al. Circ_0002395 promotes aerobic glycolysis and proliferation in pancreatic adenocarcinoma cells via miR-548c-3p/PDK1 axis. J Bioenerg Biomembr 56, 55–71 (2024). https://doi.org/10.1007/s10863-023-09995-2
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DOI: https://doi.org/10.1007/s10863-023-09995-2