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Solution Structure of the Second PDZ Domain of the Neuronal Adaptor X11α and its Interaction with the C-terminal Peptide of the Human Copper Chaperone for Superoxide Dismutase

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Abstract

Protection against reactive oxygen species is provided by the copper containing enzyme superoxide dismutase 1 (SOD1). The copper chaperone CCS is responsible for copper insertion into apo-SOD1. This role is impaired by an interaction between the second PDZ domain (PDZ2α) of the neuronal adaptor protein X11α and the third domain of CCS (McLoughlin et al. (2001) J. Biol. Chem., 276, 9303–9307). The solution structure of the PDZ2α domain has been determined and the interaction with peptides derived from CCS has been explored. PDZ2α binds to the last four amino acids of the CCS protein (PAHL) with a dissociation constant of 91 ± 2 μM. Peptide variants have been used to map the interaction areas on PDZ2α for each amino acid, showing an important role for the C-terminal leucine, in line with canonical PDZ-peptide interactions.

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Abbreviations

CCS:

copper chaperone for SOD1

HOAc:

acetic acid

PDZ:

post-synaptic density 95, Drosophila disks-large, Zona occludens 1

RP-HPLC:

reversed phase high-performance liquid chromatography

SOD1:

superoxide dismutase 1

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Authors and Affiliations

  1. Leiden Institute of Chemistry, Leiden University, The Netherlands

    Aude E. Duquesne, Martina de Ruijter, Jaap Brouwer, Marcellus Ubbink & Gerard W. Canters

  2. Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands

    Jan W. Drijfhout

  3. CMBI, Radboud University, Nijmegen, The Netherlands

    Sander B. Nabuurs & Chris A. E. M. Spronk

  4. Department of Biophysical Chemistry, Institute for Molecules and Materials, Radboud University, Nijmegen, The Netherlands

    Geerten W. Vuister

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  1. Aude E. Duquesne
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  2. Martina de Ruijter
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  3. Jaap Brouwer
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  4. Jan W. Drijfhout
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  8. Marcellus Ubbink
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Correspondence to Marcellus Ubbink.

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Duquesne, A.E., Ruijter, M.d., Brouwer, J. et al. Solution Structure of the Second PDZ Domain of the Neuronal Adaptor X11α and its Interaction with the C-terminal Peptide of the Human Copper Chaperone for Superoxide Dismutase. J Biomol NMR 32, 209–218 (2005). https://doi.org/10.1007/s10858-005-7333-1

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