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Bioglass/PLGA associated to photobiomodulation: effects on the healing process in an experimental model of calvarial bone defect

  • Biomaterials Synthesis and Characterization
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Abstract

Bioactive glasses (BG) are known for their ability to bond to bone tissue. However, in critical situations, even the osteogenic properties of BG may be not enough to induce bone consolidation. Thus, the enrichment of BG with polymers such as Poly (D, L-lactic-co-glycolic) acid (PLGA) and associated to photobiomodulation (PBM) may be a promising strategy to promote bone tissue healing. The aim of the present study was to investigate the in vivo performance of PLGA supplemented BG, associated to PBM therapy, using an experimental model of cranial bone defect in rats. Rats were distributed in 4 different groups (Bioglass, Bioglass/PBM, Bioglas/PLGA and BG/PLGA/PBM). After the surgical procedure to induce cranial bone defects, the pre-set samples were implanted and PBM treatment (low-level laser therapy) started (808 nm, 100 mW, 30 J/cm2). After 2 and 6 weeks, animals were euthanized, and the samples were retrieved for the histopathological, histomorphometric, picrosirius red staining and immunohistochemistry analysis. At 2 weeks post-surgery, it was observed granulation tissue and areas of newly formed bone in all experimental groups. At 6 weeks post-surgery, BG/PLGA (with or without PBM) more mature tissue around the biomaterial particles. Furthermore, there was a higher deposition of collagen for BG/PLGA in comparison with BG/PLGA/PBM, at second time-point. Histomorphometric analysis demonstrated higher values of BM.V/TV for BG compared to BG/PLGA (2 weeks post-surgery) and N.Ob/T.Ar for BG/PLGA compared to BG and BG/PBM (6 weeks post-surgery). This current study concluded that the use of BG/PLGA composites, associated or not to PBM, is a promising strategy for bone tissue engineering.

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Acknowledgements

The authors would like to acknowledge funding agencies FAPESP (grant number: 2014/20546-0) and CNPq for the financial support of this research and CAPES for scholarship to AMPM. In addition, the authors would like to thank Dr Ingrid Regina Avanzi for helping during some euthanasia of the animals and Prof. Dr Flavia de Oliveira and Hananiah Tardivo Quintana for assistance with picrosirius analysis.

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Authors and Affiliations

  1. Federal University of São Paulo (UNIFESP), Rua Silva Jardim, 136, Santos, SP, 11015020, Brazil

    Angela Maria Paiva Magri, Kelly Rossetti Fernandes, Hueliton Wilian Kido, Gabriela Sodano Fernandes, Stephanie de Souza Fermino, Paulo Roberto Gabbai-Armelin, Cíntia Pereirade Góes, José Lucas dos Santos Prado, Renata Neves Granito & Ana Claudia Muniz Rennó

  2. University Center of the Guaxupé Educational Foundation (UNIFEG), Avenida Dona Floriana, Guaxupé, MG, 37800000, Brazil

    Angela Maria Paiva Magri

  3. Consulmat LTDA, Rua Juan Lopes, 159, São Carlos, SP, 13567-020, Brazil

    Franscisco José Correa Braga

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Magri, A.M.P., Fernandes, K.R., Kido, H.W. et al. Bioglass/PLGA associated to photobiomodulation: effects on the healing process in an experimental model of calvarial bone defect. J Mater Sci: Mater Med 30, 105 (2019). https://doi.org/10.1007/s10856-019-6307-x

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