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Exogenous hyaluronic acid and chondroitin sulfate crosslinking treatment for increasing the amount and stability of glycosaminoglycans in bioprosthetic heart valves

  • Clinical Applications of Biomaterials
  • Original Research
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Abstract

Glutaraldehyde (GLUT) crosslinked bioprosthetic heart valves (BHVs) might fail due to progressive degradation and calcification. GLUT cannot stabilize glycosaminoglycans (GAGs), which are important for BHVs’ life time. In this current study we developed a new BHVs preparation strategy using exogenous hyaluronic acid (HA)/chondroitin sulfate (CS) supplement and sodium trimetaphosphate (STP) crosslinking method. Exogenous HA and CS provide additional GAGs for pericardiums. STP could link two GAGs by reacting with hydroxyl groups in GAGs’ repeating polysaccharides units. The feeding ratios of HA/CS were optimized. The GAGs content and long-term stability in vitro, biocompatibility, the in vivo GAGs stability and anti-calcification potential of GLUT/HA/CS and STP treated pericardiums were characterized. We demonstrated that GLUT/HA/CS and STP treated pericardiums had sufficiently increased GAGs’ amount and stability and decreased calcification. This new exogenous hyaluronic acid/chondroitin sulfate supplement and sodium trimetaphosphate crosslinking strategy would be a promising method to make BHVs with better structural stability and anti-calcification properties.

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Acknowledgements

This work was supported by National Natural Science Foundation of China (31700833), Young Elite Scientists Sponsorship Program by CAST (2017QNRC001), the Fundamental Research Funds for the Central Universities (YJ201641), National Key Research and Development Programs (2017YFC1104200, 2016YFC1102200), and the Program of Introducing Talents of Discipline to Universities (111 Project, No. B16033). We would like to thank VENUS MEDTECH Inc. (Hangzhou, China) for providing us with fresh pericardiums.

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  1. National Engineering Research Center for Biomaterials, Sichuan University, 610064, Chengdu, China

    Yang Lei, Qinggong Ning, Yuyang Tang & Yunbing Wang

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  1. Yang Lei
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  2. Qinggong Ning
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  3. Yuyang Tang
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Correspondence to Yang Lei or Yunbing Wang.

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Lei, Y., Ning, Q., Tang, Y. et al. Exogenous hyaluronic acid and chondroitin sulfate crosslinking treatment for increasing the amount and stability of glycosaminoglycans in bioprosthetic heart valves. J Mater Sci: Mater Med 30, 38 (2019). https://doi.org/10.1007/s10856-019-6237-7

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