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Bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composite for use as an artificial dural substitute

  • Engineering and Nano-engineering Approaches for Medical Devices
  • Original Research
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Abstract

A novel bilayer knitted fabric-reinforced composite for potentially being used as a dural substitute was developed by solution infiltration of oxidized regenerated cellulose knitted fabric (ORC) with poly ε-caprolactone (PCL) solution at various concentrations ranging 10–40 g/100 mL. It was found that the density of all formulations did not differ significantly and was lower than that of the human dura. Microstructure of the samples typically comprised a bilayer structure having a nonporous PCL layer on one side and the ORC/PCL composite layer on another side. Tensile modulus and strength of the samples initially decreased with increasing PCL solution concentration for up to 20 g/100 mL and re-increased again with further increasing PCL solution concentration. Strain at break of all formulations were not significantly different. Watertight test revealed that all composites could prevent leakage at the pressure within the normal range of intracranial pressure. In vitro degradation study revealed that the weight loss percentage and change in tensile properties of all samples displayed biphasic profile comprising an initially rapid decrease and followed by a gradual decrease with incubation times afterward. Micro and macro porous channels were observed to be in situ generated in the composite layer by ORC dissolution and PCL resorption during degradation while nonporous layer remained relatively unchanged. The degradation rate was found to decrease with increasing PCL solution concentration. In vitro biocompatibility using alamar blue assay on selected samples showed that fibroblasts could attach and proliferate well at all incubation periods.

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Acknowledgments

Collaborative research project grant from the Faculty of Science and the Faculty of Medicine Ramathibodi Hospital, Mahidol University (2013–2015) was acknowledged for financially supporting this study. The authors would also like to thank W. Chokvivat for the help in cell culture experiment and National Metal and Materials Technology Center for the use of characterization instruments.

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Authors and Affiliations

  1. National Metal and Materials Technology Center (MTEC), Paholyothin Road, Klong 1, Klongluang, 12120, Pathumthani, Thailand

    Jintamai Suwanprateeb & Waraporn Suvannapruk

  2. Department of Pharmacology, Faculty of Science, Mahidol University, Rama VI Road, Rajthevee, Bangkok, 10400, Thailand

    Ticomporn Luangwattanawilai, Thunyanun Theeranattapong & Warinkarn Hemstapat

  3. Neurosurgery Unit, Surgery Department, Faculty of Medicine, Ramathibodi Hospital, Rama VI Road, Rajthevee, Bangkok, 10400, Thailand

    Sorayouth Chumnanvej

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  1. Jintamai Suwanprateeb
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Correspondence to Warinkarn Hemstapat.

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Suwanprateeb, J., Luangwattanawilai, T., Theeranattapong, T. et al. Bilayer oxidized regenerated cellulose/poly ε-caprolactone knitted fabric-reinforced composite for use as an artificial dural substitute. J Mater Sci: Mater Med 27, 122 (2016). https://doi.org/10.1007/s10856-016-5736-z

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