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Novel nano-composite biomimetic biomaterial allows chondrogenic and osteogenic differentiation of bone marrow concentrate derived cells

  • Tissue Engineering Constructs and Cell Substrates
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Abstract

In clinical orthopedics suitable materials that induce and restore biological functions together with the right mechanical properties are particularly needed for the regeneration of osteochondral lesions. For this purpose, the ideal scaffold should possess the right properties with respect to degradation, cell binding, cellular uptake, non-immunogenicity, mechanical strength, and flexibility. In addition, it should be easy to handle and serve as a template for chondrocyte and bone cells guiding both cartilage and bone formation. The aim of the present study was to estimate the chondrogenic and osteogenic capability of bone marrow concentrated derived cells seeded onto a novel nano-composite biomimetic material. These properties have been evaluated by means of histological, immunohistochemical and electron microscopy analyses. The data obtained demonstrated that freshly harvested cells obtained from bone marrow were able, once seeded onto the biomaterial, to differentiate either down the chondrogenic and osteogenic pathways as evaluated by the expression and production of specific matrix molecules. These findings support the use, for the repair of osteochondral lesions, of this new nano-composite biomimetic material together with bone marrow derived cells in a “one step” transplantation procedure.

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Acknowledgments

This work was partially supported by Grants from Progetto Regione Emilia-Romagna Università 2010–2012 “Regenerative medicine of cartilage and bone”. We thank the II Orthopedic and Traumatologic Clinic of our Institute (Director Professor Sandro Giannini) for providing us with Bone Marrow Concentrate samples. Bone Sialon Protein, osteopontin and Alkaline Phopshatase Hybridoma surnatants were obtained from the Developmental Studies Hybridoma Bank. We thank Patrizia Rappini and Graziella Salmi for their assistance in the preparation of the manuscript and Despina Kiriakidu, Massimo Gamberini and Luciano Pizzi for their technical assistance.

Conflict of interest

No potential competing interests have to be reported.

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Authors and Affiliations

  1. SSD Laboratory RAMSES, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy

    Brunella Grigolo, Carola Cavallo, Giovanna Desando, Cristina Manferdini, Gina Lisignoli & Andrea Facchini

  2. SC Laboratory of Immunorheumatology and Tissue Regeneration, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy

    Brunella Grigolo, Cristina Manferdini, Gina Lisignoli & Andrea Facchini

  3. SC Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy

    Andrea Ferrari & Nicoletta Zini

  4. CNR–National Research Council of Italy, IGM, c/o Rizzoli Orthopaedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy

    Nicoletta Zini

Authors
  1. Brunella Grigolo
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  2. Carola Cavallo
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  3. Giovanna Desando
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  4. Cristina Manferdini
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  5. Gina Lisignoli
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  6. Andrea Ferrari
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  7. Nicoletta Zini
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  8. Andrea Facchini
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Corresponding author

Correspondence to Brunella Grigolo.

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In memory of Professor Andrea Facchini who died during manuscript preparation.

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Grigolo, B., Cavallo, C., Desando, G. et al. Novel nano-composite biomimetic biomaterial allows chondrogenic and osteogenic differentiation of bone marrow concentrate derived cells. J Mater Sci: Mater Med 26, 173 (2015). https://doi.org/10.1007/s10856-015-5500-9

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  • DOI: https://doi.org/10.1007/s10856-015-5500-9

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