Skip to main content

Advertisement

SpringerLink
Log in

Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells

  • Tissue Engineering Constructs and Cell Substrates
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Carbonated apatite (CO3Ap) is the inorganic component of bone. We have proposed a new method for the fabrication of CO3Ap blocks based on a dissolution-precipitation method using a synthetic precursor. The aim of this study is to examine the effects of low crystalline CO3Ap on initial cell attachment, proliferation and osteoblastic differentiation of human bone marrow cells (hBMCs) using sintered hydroxyapatite and tissue culture plates as controls. Initial cell attachment and proliferation were assessed with a MTT assay. Expression of osteoblastic markers was examined by reverse transcription-polymerase chain reaction. XRD and FT-IR results showed formation of B-type carbonate apatite with lower crystallinity. No difference was observed for initial cell attachment between HAp and CO3Ap discs. hBMSC attached more significantly on tissue culture plate than on HAp and CO3Ap discs. The number of cells on HAp was higher than that on CO3Ap until day 7, after which the number of cells was similar. hBMSC proliferated more significantly on tissue culture plate than on HAp and CO3Ap discs. In contrast, hBMCs incubated on CO3Ap demonstrated much higher expression of osteoblastic markers of differentiation, such as type I collagen, alkaline phosphatase, osteopontin and osteocalcin, than hBMCs on HAp. On the tissue culture plate, they were not any change throughout the culture period. These results demonstrated that low crystalline CO3Ap exhibit higher osteoinductivity than HAp.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Ganoids PV, Dinopoulos H, Tsiridis E. Bone substitutes: an update. Injury. 2005;36(Suppl 3):S20–7.

    Google Scholar 

  2. Friedlaender GE. Bone grafts. The basic science rationale for clinical applications. J Bone Joint Surg Am. 1987;69:786–90.

    Google Scholar 

  3. Boyce T, Edwards J, Scarborough N. Allograft bone. The influence of processing on safety and performance. Orthop Clin North Am. 1999;30:571–81.

    Article  Google Scholar 

  4. Daculsi G, Passuti N, Martin S, Deudon C, Legeros RZ, Raher S. Macroporous calcium phosphate ceramic for long bone surgery in humans and dogs. Clinical and histological study. J Biomed Mater Res. 1990;24:379–96.

    Article  Google Scholar 

  5. Frayssinet P, Trouillet JL, Rouquet N, Azimus E, Autefage A. Osseointegration of macroporous calcium phosphate ceramics having a different chemical composition. Biomaterials. 1993;14:423–9.

    Article  Google Scholar 

  6. de Groot K. Bioceramics consisting of calcium phosphate salts. Biomaterials. 1980;1:47–50.

    Article  Google Scholar 

  7. Miyamoto Y, Ishikawa K, Takechi M, Toh T, Yuasa T, Nagayama M, Suzuki K. Basic properties of calcium phosphate cement containing atelocollagen in its liquid or powder phases. Biomaterials. 1998;19:707–15.

    Article  Google Scholar 

  8. Yuasa T, Miyamoto Y, Ishikawa K, Takechi M, Momota Y, Tatehara S, Nagayama M. Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro. Biomaterials. 2004;25:1159–66.

    Article  Google Scholar 

  9. Bhatnagar RS, Qian JJ, Wedrychowska A, Sadeghi M, Wu YM, Smith N. Design of biomimetic habitats for tissue engineering with P-15, a synthetic peptide analogue of collagen. Tissue Eng. 1999;5:53–65.

    Article  Google Scholar 

  10. Yang XB, Roach HI, Clarke NM, Howdle SM, Quirk R, Shakesheff KM, Oreffo RO. Human osteoprogenitor growth and differentiation on synthetic biodegradable structures after surface modification. Bone. 2001;29:523–31.

    Article  Google Scholar 

  11. Endres M, Hutmacher DW, Salgado AJ, Kaps C, Ringe J, Reis RL, Sittinger M, Brandwood A, Schantz JT. Osteogenic induction of human bone marrow-derived mesenchymal progenitor cells in novel synthetic polymer-hydrogel matrices. Tissue Eng. 2003;9:689–702.

    Article  Google Scholar 

  12. Donath K, Rohrer MD, Beck-Mnnagetta J. A histologic evaluation of a mandibular cross section one year after augmentation with hydroxyapatite particles. Oral Surg Oral Med Oral Pathol. 1987;63:651–5.

    Article  Google Scholar 

  13. Bauer TW, Muschler GF. Bone graft materials. An overview of the basic science. Clin Orthop Relat Res. 2000;371:10–27.

    Article  Google Scholar 

  14. Yuasa T, Miyamoto Y, Ishikawa K, Takechi M, Nagayama M, Suzuki K. In vitro resorption of three apatite cements with osteoclasts. J Biomed Mater Res. 2001;54:344–50.

    Article  Google Scholar 

  15. Doi Y, Shibutani T, Moriwaki Y, Kajimoto T, Iwayama Y. Sintered carbonate apatites as bioresorbable bone substitutes. J Biomed Mater Res. 1998;39:603–10.

    Article  Google Scholar 

  16. Hasegawa M, Doi Y, Uchida A. Cell-mediated bioresorption of sintered carbonate apatite in rabbits. J Bone Joint Surg Br. 2003;85:142–7.

  17. de Groot K. Effect of porosity and physicochemical properties on the stability, resorption and strength of calcium phosphate ceramics. Ann N Y Acad Sci. 1988;523:227–33.

    Article  Google Scholar 

  18. Rau JV, Cesaro SN, Ferro D, Barinov SM, Fadeeva IV. FTIR study of carbonate loss from carbonated apatites in the wide temperature range. J Biomed Mater Res B Appl Biomater. 2004;71:441–7.

    Article  Google Scholar 

  19. Landi E, Tampieri A, Celotti G, Vichi L, Sandri M. Influence of synthesis and sintering parameters on the characteristics of carbonate apatite. Biomaterials. 2004;25:1763–70.

    Article  Google Scholar 

  20. Lin X, Matsuya S, Udoh K, Nakagawa M, Terada Y, Ishikawa K. Fabrication of calcium carbonate monolith by carbonation of calcium hydroxide compact. Archives Bioceram Res. 2003;3:83–8.

  21. Ishikawa K, Matsuya S, Lin X, Lei Z, Yuasa T, Miyamoto Y. Fabrication of low crystalline B-type carbonate apatite block from low crystalline calcite block. J Ceram Soc Jpn. 2010;118:341–4.

    Article  Google Scholar 

  22. Wakae H, Takeuchi A, Udoh K, Matsuya S, Munar ML, LeGeros RZ, Nakasima A, Ishikawa K. Fabrication of macroporous carbonate apatite foam by hydrothermal conversion of alpha-tricalcium phosphate in carbonate solutions. J Biomed Mater Res A. 2008;87:957–63.

    Article  Google Scholar 

  23. Ishikawa K. Bone substitute fabrication based on dissolution-precipitation reactions. Materials. 2010;3:1138–55.

    Article  Google Scholar 

  24. Lin X, Matsuya S, Nakagawa M, Terada Y, Ishikawa K. Effect of molding pressure on fabrication of low-crystalline calcite block. J Mater Sci Mater Med. 2008;19:479–84.

    Article  Google Scholar 

  25. Dias AG, Lopes MA, Trigo Cabral AT, Santos JD, Fernandes MH. In vitro studies of calcium phosphate glass ceramics with different solubility with the use of human bone marrow cells. J Biomed Mater Res A. 2005;74:347–55.

    Article  Google Scholar 

  26. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143–7.

    Article  Google Scholar 

  27. Beloti MM, Hiraki KR, Barros VM, Rosa AL. Effect of the chemical composition of Ricinus communis polyurethane on rat bone marrow cell attachment, proliferation, and differentiation. J Biomed Mater Res. 2003;64:171–6.

    Article  Google Scholar 

  28. Aubin JE. Regulation of osteoblast formation and function. Rev Endocr Metab Disord. 2001;2:81–94.

    Article  Google Scholar 

  29. Neve A, Corrado A, Cantatore FP. Osteoblast physiology in normal and pathological conditions. Cell Tissue Res. 2011;343:289–302.

    Article  Google Scholar 

  30. Lian JB, Stein GS, Stein JL, van Wijnen AJ. Regulated expression of the bone specific osteocalcin gene by vitamins and hormones. Vitam Horm. 1999;55:443–509.

    Article  Google Scholar 

  31. Owen TA, Aronow M, Shalhoub V, Barone LM, Wilning L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS. Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol. 1990;143:420–30.

    Article  Google Scholar 

  32. Stein GS, Lian JB, Owen TA. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEB J. 1990;4:3111–23.

    Google Scholar 

  33. Owen ME, Cave J, Joyner CJ. Clonal analysis in vitro of osteogenic differentiation of marrow CFU-F. J Cell Sci. 1987;87:731–8.

    Google Scholar 

  34. Alliot-Licht B, Gregoire M, Orly I, Menanteau J. Cellular activity of osteoblasts in the presence of hydroxyapatite: an in vitro experiment. Biomaterials. 1991;12:752–6.

    Article  Google Scholar 

  35. Ozawa S, Kasugai S. Evaluation of implant materials (hydroxyapatite, glass-ceramics, titanium) in rat bone marrow stromal cell culture. Biomaterials. 1996;17:23–9.

    Article  Google Scholar 

  36. Hauschka PV, Lian JB, Cole DEC, Gundberg CM. Osteocalcin and matrix gla protein: vitamin K-dependent proteins in bone. Physiol Rev. 1989;69:990–1047.

    Google Scholar 

  37. Chenu C, Colucci S, Grano M, Zigrino P, Barattolo R, Zambonin G, Baldini N, Vergnaud P, Delmas PD, Zallone AZ. Osteocalcin induces chemotaxis, secretion of matrix proteins, and calcium-mediated intracellular signaling in human osteoclast-like cells. J Cell Biol. 1994;127:1149–58.

    Article  Google Scholar 

  38. Liggett WH, Lian JB, Greenberger JS, Glowacki J. Osteocalcin promotes differentiation of putative osteoclast progenitors from murine long-term bone marrow cultures. J Cell Biochem. 1994;55:190–9.

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported in part by a Grant-in-Aid for Scientific Research (C) (24592989).

Author information

Authors and Affiliations

  1. Subdivision of Molecular Oral Medicine, Division of Integrated Sciences of Translational Research, Department of Oral Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima, 770-8504, Japan

    Hirokazu Nagai, Masako Kobayashi-Fujioka, Kenji Fujisawa, Go Ohe, Natsumi Takamaru, Kanae Hara, Daisuke Uchida, Tetsuya Tamatani & Youji Miyamoto

  2. Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, 812-8582, Japan

    Kunio Ishikawa

Authors
  1. Hirokazu Nagai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masako Kobayashi-Fujioka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kenji Fujisawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Go Ohe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Natsumi Takamaru
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kanae Hara
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Daisuke Uchida
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Tetsuya Tamatani
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Kunio Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Youji Miyamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hirokazu Nagai.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nagai, H., Kobayashi-Fujioka, M., Fujisawa, K. et al. Effects of low crystalline carbonate apatite on proliferation and osteoblastic differentiation of human bone marrow cells. J Mater Sci: Mater Med 26, 99 (2015). https://doi.org/10.1007/s10856-015-5431-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s10856-015-5431-5

Keywords

Search

Navigation