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An alternative to nerve repair using an antioxidant compound: a histological study in rats

  • Biocompatibility Studies
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Abstract

The fascicular composition and organisation of the inferior alveolar nerve (IAN) were determined to confirm the microarchitecture of the IAN bundles into each of the mandibular teeth, including the composition of the mental nerve. The aim of this study was to evaluate peripheral nerve repair after the application of an antioxidant compound to the damaged nerve tissue to elevate the concentration and bioavailability of elements capable of favouring tissue repair. Twenty-five Wistar rats were divided into groups: The Control 1 (Ctl 1) (n = 5) animals had the ischiatic nerve exposed with no suture injury and were sacrificed at 30 days post-operatively. The Control 2 (Ctl 2) (n = 10) animals had the ischiatic nerve exposed, and the nerve was injured using suture in three distinct regions. In the experimental (Exp) animals (n = 10), an antioxidant organic compound was applied to the nerve injury site. The animals with nerve injury (Ctl2 and Exp group) were sacrificed at 15 and 30 days post-operatively. The histological analysis showed less degeneration in the Exp group at 15 and 30 days post-operatively. Nerve neoformation forming a connection between the distal and proximal suture sites was observed in the experimental group. This study presented an alternative to nerve repair using an antioxidant compound.

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Acknowledgments

We express our gratitude to Dra. Maria Inês Nogueira from the Laboratory of Neurosciences ICB/USP for collaboration in the data collection.

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Authors and Affiliations

  1. Department of Health Sciences - School of Dentistry, 9 de Julho University, São Paulo, SP, Brazil

    Marcos B. Salles & Elio H. Shinohara

  2. Biotecnos Research Center, Rua Dr. Bozano, 571 - CP 97015-001, Santa Maria (RS), Brazil

    Sergio A. Gehrke

  3. Catholic University of Uruguay, Montevideo, Uruguay

    Sergio A. Gehrke

  4. Division of Periodontology, Department of Oral Medicine, Infection and Immunity Harvard School of Dental Medicine, Boston, MA, USA

    Samuel Koo

  5. Institute of Nuclear Energy Research (IPEN), Science and Technology Materials Center (CCTM), São Paulo, Brazil

    Sergio Allegrini Jr., Sizue O. Rogero & Marcelo Yoshimoto

  6. Institute Adolfo Lutz, Laboratory of Cell Culture, São Paulo, Brazil

    Tamiko I. Ikeda & Áurea S. Cruz

  7. Graduate Program in Biodentistry, Ibirapuera University (UNIB), São Paulo, SP, 04661 100, Brazil

    Sergio Allegrini Jr. & Marcelo Yoshimoto

Authors
  1. Marcos B. Salles
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  2. Sergio A. Gehrke
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  3. Samuel Koo
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  4. Sergio Allegrini Jr.
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  6. Tamiko I. Ikeda
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  8. Elio H. Shinohara
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  9. Marcelo Yoshimoto
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Correspondence to Sergio A. Gehrke.

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Salles, M.B., Gehrke, S.A., Koo, S. et al. An alternative to nerve repair using an antioxidant compound: a histological study in rats. J Mater Sci: Mater Med 26, 14 (2015). https://doi.org/10.1007/s10856-014-5340-z

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  • DOI: https://doi.org/10.1007/s10856-014-5340-z

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