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Antimicrobial and in vitro wound healing properties of novel clay based bionanocomposite films

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Abstract

The present study investigates the development of methyl cellulose (MC)–sodium alginate (SA)–montmorillonite (MMT) clay based bionanocomposite films with interesting wound healing properties. The differential scanning calorimetry analysis of the composite films revealed presence of single glass transition temperature (Tg) confirming the miscible nature of the ternary blended films. The increase in MMT ratio in the composite films reduced the mobility of biopolymer chains (MC/SA) which increased the Tg of the film. Thermogravimetric analysis showed that dispersion of clay (MMT) at nano level significantly delayed the weight loss that correlated with higher thermal stability of the composite films. It was observed that the developed films were able to exhibit antimicrobial activity against four typical pathogenic bacteria found in the presence of wound. The developed films were able to significantly inhibit (10 mg/ml) the growth of Enterococcus faecium and Pseudomonas aeruginosa. In vitro scratch assay indicated potential wound closure activities of MC-2–4 bionanocomposite films at their respective highest subtoxic doses. In conclusion, these ternary bionanocomposite films were found to be promising systems for wound healing applications.

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Acknowledgments

The Universiti Teknologi MARA (UiTM) is thanked for the financial support under the Research Intensive Fund (RIF) 600 RMI/DANA 5/3/RIF (734/2012) and Long Term Research Grant Scheme (600-RMI/LRGS 5/3, 2/2012).

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Authors and Affiliations

  1. Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), 42300, Bandar Puncak Alam, Selangor, Malaysia

    R. K. Mishra, K. Ramasamy, S. M. Lim, M. F. Ismail & A. B. A. Majeed

  2. Brain Degeneration and Therapeutics Group, Brain and Neuroscience Communities of Research, Universiti Teknologi MARA (UiTM), 40450, Shah Alam, Selangor Darul Ehsan, Malaysia

    R. K. Mishra & A. B. A. Majeed

  3. Collaborative Drug Discovery Research (CDDR) Group, Brain and Neuroscience Communities of Research, Universiti Teknologi MARA (UiTM), 40450, Shah Alam, Selangor Darul Ehsan, Malaysia

    K. Ramasamy, S. M. Lim & M. F. Ismail

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  1. R. K. Mishra
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Mishra, R.K., Ramasamy, K., Lim, S.M. et al. Antimicrobial and in vitro wound healing properties of novel clay based bionanocomposite films. J Mater Sci: Mater Med 25, 1925–1939 (2014). https://doi.org/10.1007/s10856-014-5228-y

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