Skip to main content

Advertisement

SpringerLink
Log in

Osteogenic activity of silver-loaded coral hydroxyapatite and its investigation in vivo

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

In this study, the scaffolds based on mineralized silver-loaded coral hydroxyapatites (SLCHAs) were developed for bone regeneration in the radius of rabbit with a 15-mm infective segmental defect model for the first time. The SLCHAs were achieved by surface adsorption and ion-exchange reaction between Ca2+ of coral hydroxyapatite (CHA) and Ag+ of silver nitrate with different concentration at room temperature. Release experiment in vitro, X-ray diffraction and scanning electron microscopy equipped with energy-dispersive X-ray spectrometer were applied to exhibit that the scaffold showed some features of natural bone both in main component and hierarchical microstructure. The three-dimensional porous scaffold materials imitate the microstructure of cancellous bone. Mouse embryonic pre-osteoblast cells (MC3T3-E1) were used to investigate the cytocompatibility of SLCHAs, CHA and pure coral. Cell activity were studied with alkaline phosphataseenzyme assay after 2, 4, 6 days of incubation. It was no statistically significant differences in cell activity on the scaffolds of Ag+(13.6 μg/mL)/CHA, Ag+(1.7 μg/mL)/CHA, CHA and pure coral. The results indicated that the lower silver concentration has little effect on cell activity. In the implantation test, the infective segmental defect repaired with SLCHAs was healed up after 10 weeks after surgery, and the implanted composites were almost substituted by new bone tissue, which were very comparable with the scaffold based on mineralized CHA. It could be concluded that the SLCHAs contained with appropriate silver ionic content could act as biocidal agents and maintain the advantages of mineralized CHA or coral, while avoiding potential bacteria-dangers and toxical heavy-metal reaction. All the above results showed that the SLCHAs with anti-infective would be as a promising scaffold material, which whould be widely applied into the clinical for bone regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Foo LS, Hardes J, Henrichs M, Ahrens H, Gosheger G, Streitbürger A. Surgical difficulties encountered with use of modular endoprosthesis for limb preserving salvage of failed allograft reconstruction after malignant tumor resection. J Arthroplasty. 2011;26:744.

    Article  Google Scholar 

  2. Garcia-Cimbrelo E, Garcia-Rey E, Cruz-Pardos A. The extent of the bone defect affects the outcome of femoral reconstruction in revision surgery with impacted bone grafting: a 5–17-year follow-up study. J Bone Joint Surg Br. 2011;93:1457.

    Article  Google Scholar 

  3. Paderni S, Terzi S, Amendola L. Major bone defect treatment with an osteoconductive bone substitute. Chir Organi Mov. 2009;93:89.

    Google Scholar 

  4. Steinlechner CW, Mkandawire NC. Non-vascularised fibular transfer in the management of defects of long bones after sequestrectomy in children. J Bone Joint Surg Br. 2005;87:1259.

    Article  Google Scholar 

  5. DeCoster TA, Gehlert RJ, Mikola EA, Pirela-Cruz MA. Management of posttraumatic segmental bone defects. J Am Acad Orthop Surg. 2004;12:28.

    Google Scholar 

  6. Raucci MG, D’Antò V, Guarino V, Sardella E, Zeppetelli S, Favia P, Ambrosio L. Biomineralized porous composite scaffolds prepared by chemical synthesis for bone tissue regeneration. Acta Biomater. 2010;6:4090.

    Article  Google Scholar 

  7. Giannoudis PV, Chris Arts JJ, Schmidmaier G, Larsson S. What should be the characteristics of the ideal bone graft substitute? Injury. 2011;42:S1.

    Google Scholar 

  8. Khaled EG, Saleh M, Hindocha S, Griffin M, Khan WS. Tissue engineering for bone production- stem cells, gene therapy and scaffolds. Open Orthop J. 2011;5:289.

    Article  Google Scholar 

  9. Rohanizadeh R, Chung K. Hydroxyapatite as a carrier for bone morphogenetic protein. J Oral Implantol. 2011;37:659.

    Article  Google Scholar 

  10. Mishra S, Rajyalakshmi A, Balasubramanian K. Compositional dependence of hematopoietic stem cells expansion on bioceramic composite scaffolds for bone tissue engineering. J Biomed Mater Res A. 2012;100:2483.

    Google Scholar 

  11. He J, Huang T, Gan L, Zhou Z, Jiang B, Wu Y, Wu F, Gu Z. Collagen-infiltrated porous hydroxyapatite coating and its osteogenic properties: in vitro and in vivo study. J Biomed Mater Res A. 2012;100:1706.

    Article  Google Scholar 

  12. Lee JH, Yu CH, Yang JJ, Baek HR, Lee KM, Koo TY, Chang BS, Lee CK. Comparative study of fusion rate induced by different dosages of Escherichia coli-derived recombinant human bone morphogenetic protein-2 using hydroxyapatite carrier. Spine J. 2012;12:239.

    Article  Google Scholar 

  13. Seeherman HJ, Azari K, Bidic S, Rogers L, Li XJ, Hollinger OJ, Wozney JM. rhBMP-2 delivered in a calcium phosphate cement accelerates bridging of critical-sized defects in rabbit radii. J Bone Joint Surg Am. 2006;88:1553.

    Article  Google Scholar 

  14. Cai L, Wang Q, Gu C, Wu J, Wang J, Kang N, Hu J, Xie F, Yan L, Liu X, Cao Y, Xiao R. Vascular and micro-environmental influences on MSC-coral hydroxyapatite construct-based bone tissue engineering. Biomaterials. 2011;32:8497.

    Article  Google Scholar 

  15. Figueiredo M, Henriques J, Martins G, Guerra F, Judas F, Figueiredo H. Physicochemical characterization of biomaterials commonly used in dentistry as bone substitutes–comparison with human bone. J Biomed Mater Res B. 2010;92:409.

    Google Scholar 

  16. Ripamonti U, Crooks J, Khoali L, Roden L. The induction of bone formation by coral-derived calcium carbonate/hydroxyapatite constructs. Biomaterials. 2009;30:1428.

    Article  Google Scholar 

  17. Alexander JW. History of the medical use of silver. Surg Infect. 2009;10:289.

    Article  Google Scholar 

  18. Lo SF, Hayter M, Chang CJ, Hu WY, Lee LL. A systematic review of silver-releasing dressings in the management of infected chronic wounds. J Clin Nurs. 2008;17:1973.

    Article  Google Scholar 

  19. Ghani Y, Coathup MJ, Hing KA, Blunn GW. Development of a hydroxyapatite coating containing silver for the prevention of peri-prosthetic infection. J Orthop Res. 2012;30:356.

    Article  Google Scholar 

  20. Vulpoi A, Gruian C, Vanea E, Baia L, Simon S, Steinhoff HJ, Göller G, Simon V. Bioactivity and protein attachment onto bioactive glasses containing silver nanoparticles. J Biomed Mater Res A. 2012;100:1179.

    Article  Google Scholar 

  21. Paladini F, Pollini M, Talà A, Alifano P, Sannino A. Efficacy of silver treated catheters for haemodialysis in preventing bacterial adhesion. J Mater Sci Mater Med. 2012;23:1983.

    Article  Google Scholar 

  22. Kowalczuk D, Ginalska G, Piersiak T, Miazga-Karska M. Prevention of biofilm formation on urinary catheters: comparison of the sparfloxacin-treated long-term antimicrobial catheters with silver-coated ones. J Biomed Mater Res B. 2012;100B:1874.

    Article  Google Scholar 

  23. Zhang Y, Yin QS, Zhang Y, Xia H, Ai FZ, Jiao YP, Chen XQ. Determination of antibacterial properties and cytocompatibility of silver-loaded coral hydroxyapatite. J Mater Sci Mater Med. 2010;21:2453.

    Article  Google Scholar 

  24. Ales H, Lenka J, Ludvik S. The influence of simulated body fluid composition on carbonated hydroxyapatite formation. Ceramics. 2002;46:9–14.

    Google Scholar 

  25. Oyane A, Onuma K, Ito A, Kim HM, Kokubo T, Nakamura T. Formation and growth of clusters in conventional and new kinds of simulated body fluids. J Biomed Mater Res. 2003;64A:339–48.

    Article  Google Scholar 

  26. Vecchio KS, Zhang X, Massie JB, Wang M, Kim CW. Conversion of sea urchin spines to Mg-substituted tricalcium phosphate for bone implants. Acta Biomater. 2007;3:785.

    Article  Google Scholar 

  27. Douglas T, Pamula E, Hauk D, Wiltfang J, Sivananthan S, Sherry E, Warnke PH. Porous polymer/hydroxyapatite scaffolds: characterization and biocompatibility investigations. J Mater Sci Mater Med. 2009;20:1909.

    Article  Google Scholar 

  28. Guda T, Walker JA, Pollot BE, Appleford MR, Oh S, Ong JL, Wenke JC. In vivo performance of bilayer hydroxyapatite scaffolds for bone tissue regeneration in the rabbit radius. J Mater Sci Mater Med. 2011;22:647.

    Article  Google Scholar 

Download references

Acknowledgments

This work was in part supported financially by a grant from the program for Science and Technology Key of Guangdong Province (2003C104004 & 2004B33101005), Plan of 973 (2012CB619106) and twelfth five-year plan of military (CWS11C268) in China, the Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials in China and the analysis foundation of Tsinghua University.

Author information

Authors and Affiliations

  1. Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, People’s Republic of China

    Yu Zhang, Qing-Shui Yin, Hong Xia & Ying Zhang

  2. Department of Orthopaedics, Zhu-Jiang Hospital of Southern Medical University (First Military Medical University), Guangzhou, 510282, People’s Republic of China

    Chu-Song Zhou

  3. Department of Materials Science and Engineering, Jinan University, Guangzhou, 510630, People’s Republic of China

    Yan-Peng Jiao

Authors
  1. Yu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qing-Shui Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chu-Song Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hong Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ying Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yan-Peng Jiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Chu-Song Zhou, Hong Xia or Ying Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Y., Yin, QS., Zhou, CS. et al. Osteogenic activity of silver-loaded coral hydroxyapatite and its investigation in vivo. J Mater Sci: Mater Med 25, 801–812 (2014). https://doi.org/10.1007/s10856-013-5115-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-013-5115-y

Keywords

Search

Navigation