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Effect of chitosan-gluconic acid conjugate/poly(vinyl alcohol) cryogels as wound dressing on partial-thickness wounds in diabetic rats

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Abstract

We previously developed chitosan cryogels from chitosan-gluconic acid conjugate without using toxic additives for wound care. In this study, we improved physiological characteristics of the previous cryogels by incorporating poly(vinyl alcohol) that also form cryogels. Mechanical strength of the cryogels was more than two times higher than that of the previous cryogels. Furthermore, the incorporation of poly(vinyl alcohol) enhanced water retention and resistance to degradation of the gels by lysozyme. The cryogels retained the favorable biological properties of the previous cryogels that they accelerate infiltration of inflammatory cells into wound sites. Time period for repairing 50 % of initial area of partial-thickness skin wound treated with the cryogels (4.0 ± 1.1 days) was shorter than those with gauze (6.5 ± 0.3 days) or a commercial hydrogel dressing (5.7 ± 0.3 days). Finally, we confirmed that incorporation of basic fibroblast growth factor into the cryogels was effective to further accelerate wound healing (2.7 ± 1.0 days). These results demonstrate that the cryogels in this study are promising for wound care.

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Acknowledgments

This research was partially supported by the Adaptable and Seamless Technology Transfer Program through Target-driven R&D, Japan Science and Technology Agency (AS231Z03302F).

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Authors and Affiliations

  1. Department of Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima, 890-0065, Japan

    Takayuki Takei & Masahiro Yoshida

  2. Department of Chemical Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0385, Japan

    Hideki Nakahara & Koei Kawakami

  3. Department of Gastroenterology, Nanpuh Hospital, 14-3 Nagata-cho, Kagoshima, 891-8512, Japan

    Sadao Tanaka

  4. Department of Pathology, Nanpuh Hospital, 14-3 Nagata-cho, Kagoshima, 891-8512, Japan

    Hiroto Nishimata

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  1. Takayuki Takei
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  2. Hideki Nakahara
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  3. Sadao Tanaka
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  4. Hiroto Nishimata
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  5. Masahiro Yoshida
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  6. Koei Kawakami
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Correspondence to Takayuki Takei.

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Takei, T., Nakahara, H., Tanaka, S. et al. Effect of chitosan-gluconic acid conjugate/poly(vinyl alcohol) cryogels as wound dressing on partial-thickness wounds in diabetic rats. J Mater Sci: Mater Med 24, 2479–2487 (2013). https://doi.org/10.1007/s10856-013-4991-5

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  • DOI: https://doi.org/10.1007/s10856-013-4991-5

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