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PolyNaSS grafting on titanium surfaces enhances osteoblast differentiation and inhibits Staphylococcus aureus adhesion

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Abstract

Titanium surface modifications to simultaneously prevent bacterial adhesion but promote bone-cell functions could be highly beneficial for improving implant osseointegration. In the present in vitro study, the effect of sulfonate groups on titanium surfaces was investigated with respect to both S. aureus adhesion and osteoblast functions pertinent to new bone formation. Commercial pure titanium (cpTi) squares were oxydized (Tiox), grafted with poly(sodium styrene sulfonate) groups (Tigraft) by covalent bonding using radical polymerization, and were characterized by infrared spectroscopy (HATR-FTIR) and colorimetry. Bacterial adhesion study showed that Tigraft exhibited high inhibition of S. aureus adhesion S at levels >90 %, when compared to cpTi (P < 0.05). In contrast osteoblasts adhesion was similar on all three titanium surfaces. While the kinetics of cell proliferation were similar on the three titanium surfaces, Alkaline phosphatase-specific activity of osteoblasts cultured on Tigraft surfaces was twofold higher than that observed on either on Tiox or cpTi surfaces (P < 0.01). More importantly, the amount and the distribution of calcium-containing nodules was different. The total area covered by calcium-containing nodules was 2.2-fold higher on the Tigraft as compared to either Tiox or cpTi surfaces (P < 0.01). These results provide evidence that poly(sodium styrene sulfonate) groups grafting on cpTi simultaneously inhibits bacteria adhesion but promote osteoblast function pertinent to new bone formation. Such modified titanium surfaces offer a promising strategy for preventing biofilm-related infections and enhancing osteointegration of implants in orthopaedic and dental applications.

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Acknowledgments

We gratefully acknowledge the help of Prof Rena Bizios for critical review of the paper.

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Author notes

  1. G. Pavon-Djavid

    Present address: Laboratoire de Bio-ingénierie de Polymères Cardiovasculaires, INSERM U698, Institut Galilée, Université Paris 13, PRES Sorbonne Paris Cité, Villetaneuse, France

Authors and Affiliations

  1. Laboratoire de Bio ingénierie et Biomécanique Ostéoarticulaires, UMR CNRS 7052, 10, Avenue de Verdun, 75010, Paris, France

    A. Alcheikh, H. Petite & F. Anagnostou

  2. Laboratoire de Biomatériaux et Polymères de Spécialité, CSPBAT UMR CNRS 7244, Villetaneuse, France

    G. Pavon-Djavid, G. Helary & V. Migonney

  3. Service of Odontology, Pitié Salpetrière Hospital, Department of Periodontology, U.F.R. of Odontology, Paris 7-Denis Diderot University, PRES Sorbonne Paris Cité, Paris, France

    F. Anagnostou

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  1. A. Alcheikh
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Correspondence to F. Anagnostou.

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V. Migonney and F. Anagnostou both authors contributed equally to this work

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Alcheikh, A., Pavon-Djavid, G., Helary, G. et al. PolyNaSS grafting on titanium surfaces enhances osteoblast differentiation and inhibits Staphylococcus aureus adhesion. J Mater Sci: Mater Med 24, 1745–1754 (2013). https://doi.org/10.1007/s10856-013-4932-3

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  • DOI: https://doi.org/10.1007/s10856-013-4932-3

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