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Chitosan/poly(dl,lactide-co-glycolide) scaffolds for tissue engineering

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Abstract

Chitosan/poly(dl-lactide-co-glycolide) (Ch/dl PLG) composite scaffolds were fabricated by freeze-drying lyophilization, and were evaluated and compared for use as a bone regeneration scaffold through measurements of the compression mechanical properties of the porous scaffolds. Also, In vitro cell culture of Sprague–Dawley rat’s osteoblasts were used to evaluate the phenotype expression of cells in the scaffolds, characterizing the cellular adhesion, proliferation and alkaline phosphatase activity. The gene expression of osteocalcin, sialoprotein, alkaline phosphatase, Type I collagen and TGFβ1 were confirmed in the samples; moreover, it was confirmed, the mineralization by IR spectra and EDS analysis. Our results thus show that Ch/dl PLG scaffolds are suitable for biological applications.

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Acknowledgments

The authors acknowledge the financial support of the Mexican Public Education Secretary (SEP) and the Mexican National Council for Science and Technology (CONACyT) through project SEP-CONACyT 2012-180909 and PROMEP 103.5/10/4995 UACJ-EXB-156. Also, we greatly appreciate the support of Christian Chapa and Monica Duarte during the SEM/EDS, and mechanical characterization. We are extremely grateful for the advice and assistance of Manuel Alejandro Hernández-Aguilar, B.S., Idahí Gutiérrez-Ornelas B.S., and Laura De la Rosa, PhD, during the cell culture.

Conflict of interest declaration

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Instituto de Ingeniería y Tecnología, Universidad Autónoma de Cd. Juárez, Av. Del Charro 450 Norte, Col. Partido Romero, 32310, Cd. Juárez, CHIH, Mexico

    S. A. Martel-Estrada, I. Olivas-Armendáriz, C. A. Martínez-Pérez & P. E. García-Casillas

  2. Physics Department, University of Wisconsin-Madison, 1150 University Ave, Madison, WI, 53706, USA

    T. Hernández

  3. Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Henri Dunant 4016, Zona Pronaf, 32310, Cd. Juárez, CHIH, Mexico

    E. I. Acosta-Gómez & F. Jiménez-Vega

  4. Departamento de Integridad y Desarrollo de Materiales Compuestos, Centro de Investigación en Materiales Avanzados, Miguel de Cervantes 120, Complejo Industrial Chihuahua, 31109, Chihuahua, Mexico

    J. G. Chacón-Nava

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  1. S. A. Martel-Estrada
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  2. I. Olivas-Armendáriz
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  3. C. A. Martínez-Pérez
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  4. T. Hernández
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  5. E. I. Acosta-Gómez
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  6. J. G. Chacón-Nava
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  7. F. Jiménez-Vega
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  8. P. E. García-Casillas
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Correspondence to S. A. Martel-Estrada.

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Martel-Estrada, S.A., Olivas-Armendáriz, I., Martínez-Pérez, C.A. et al. Chitosan/poly(dl,lactide-co-glycolide) scaffolds for tissue engineering. J Mater Sci: Mater Med 23, 2893–2901 (2012). https://doi.org/10.1007/s10856-012-4762-8

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  • DOI: https://doi.org/10.1007/s10856-012-4762-8

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