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Laser-engineered topography: correlation between structure dimensions and cell control

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Abstract

Topographical cues have a significant impact on cell responses and by this means, on the fabrication of innovative implant materials. However, analysis of cell-topography interactions in dependence of the surface feature dimensions is still challenging due to limitations in the fabrication technology. Here, we introduce surface structuring via picosecond laser systems, which enable a fast production of micro-sized topologies. Changes in the processing parameters further control the feature sizes of so-called spikes. Using surfaces with big and small spike-to-spike-distances for comparisons, we focussed on cell adhesion via extracellular matrix adsorption and focal adhesion complexes, morphology, localisation and proliferation of fibroblasts. The observed cell control was dependent on a turnover point related to the structure dimensions: only big spike-to-spike-distances reduced cell behaviour. Therefore, this technology offers a platform to study cell and tissue interactions with a defined microenvironment.

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Acknowledgments

This work was partly supported by Cluster of Excellence Rebirth “From Regenerative Biology to Reconstructive Therapy” and BMBF-project REMEDIS. The authors thank Prof. Dr. H. Küster, head of the Institute of Biophysics (Leibniz University Hannover, Germany) for granting the use of the microplate reader and fluorescence microscope.

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Authors and Affiliations

  1. Laser Zentrum Hannover e.V., Hollerithallee 8, 30419, Hannover, Germany

    Sabrina Schlie, Elena Fadeeva, Anastasia Koroleva & Boris N. Chichkov

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  1. Sabrina Schlie
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  2. Elena Fadeeva
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  3. Anastasia Koroleva
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  4. Boris N. Chichkov
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Correspondence to Sabrina Schlie.

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Schlie, S., Fadeeva, E., Koroleva, A. et al. Laser-engineered topography: correlation between structure dimensions and cell control. J Mater Sci: Mater Med 23, 2813–2819 (2012). https://doi.org/10.1007/s10856-012-4737-9

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  • DOI: https://doi.org/10.1007/s10856-012-4737-9

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