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In vivo effects of isolated implantation of salmon-derived crosslinked atelocollagen sponge into an osteochondral defect

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Abstract

We have developed crosslinked salmon-derived atelocollagen (SC) sponge, which has a denaturation temperature of 47°C. Sixty-four knees of 32 mature rabbits were randomly divided into 4 groups after creating an osteochondral defect in the femoral trochlea. Defects in Groups I, II, and III were filled with the crosslinked SC sponge, the crosslinked porcine collagen (PC) sponge, and the non-crosslinked PC sponge, respectively. In Group IV, defects were left untreated as the control. At 12 weeks after implantation, the histological score showed that Group I was significantly greater than Groups III (P = 0.0196) and IV (P = 0.0021). In addition, gene expression of type-2 collagen, aggrecan, and SOX9 was the greatest in Group I at 12 weeks. The fundamental in vivo properties of the crosslinked SC sponge showed that this is a promising biomaterial, specifically as a scaffold for cartilage tissue engineering.

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Acknowledgments

We thank Yuko Taniguro for assistance with the animal experiments, and Dr. Yoshie Tanabe for data collection. This work was financially supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and from Takeda Science Foundation, Japan.

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Authors and Affiliations

  1. Department of Sports Medicine and Joint Surgery, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan

    Yasuyuki Kawaguchi, Eiji Kondo, Nobuto Kitamura, Kazunobu Arakaki & Kazunori Yasuda

  2. Department of Orthopaedic Surgery, Nara Medical University, Kashihara, Nara, Japan

    Yasuyuki Kawaguchi & Yasuhito Tanaka

  3. Division of Biotechnology and Macromolecular Chemistry, Hokkaido University Graduate School of Engineering, Sapporo, Japan

    Masanobu Munekata

  4. Department of Translational Research, Center for Translational and Advanced Animal Research, Tohoku University Graduate School of Medicine, Sendai, Japan

    Nobuhiro Nagai

Authors
  1. Yasuyuki Kawaguchi
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  2. Eiji Kondo
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  3. Nobuto Kitamura
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  4. Kazunobu Arakaki
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  5. Yasuhito Tanaka
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  6. Masanobu Munekata
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  7. Nobuhiro Nagai
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  8. Kazunori Yasuda
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Correspondence to Kazunori Yasuda.

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Kawaguchi, Y., Kondo, E., Kitamura, N. et al. In vivo effects of isolated implantation of salmon-derived crosslinked atelocollagen sponge into an osteochondral defect. J Mater Sci: Mater Med 22, 397–404 (2011). https://doi.org/10.1007/s10856-010-4215-1

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  • DOI: https://doi.org/10.1007/s10856-010-4215-1

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